Objective To investigate the effects of adenosine receptor A2a on the expression of epithelial sodium channel ?-subunit (?-ENaC) in alveolar epithelium A549 cells and the effects of adenosine receptor A2a in acute lung injury/aute respiratory distress syndrome. Methods After alveolar epithelium A549 Cells were incubated with 0,0.1,1,10 and 100 ?mol/L adenosine receptor A2a agonist CGS-21680 for 8 h or with 100 ?mol/L CGS-21680 for 0,1,4,8,24 and 48 h respectively,the mRNA and protein levels of ?-ENaC were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis respectively. Results After A549 cells were incubated with CGS-21680 at different doses for 8 h,the mRNA and protein levels of ?-ENaC were elevated significantly at 0.1 ?mol/L CGS-21680 treatment compared with the control group (P

0.05).The ENaC-? mRNA and protein expression level in A549 cells in 6,12 and 24 h after treatment with ulinastatin (at the concentration of 5 000 U/ml) + TNF-? was significantly increased compared with that after treatment with TNF-? (P

To improve the training quality of medical students,the questionnaire investigation was carried out to evaluate the effects of early clinic education course,which has provided us with the evidence to amend the teaching contents,teaching model,teaching methods.

Objective To investigate the changes of thyroid hormone levels and its relationship with prognosis in old patients with sepsis. Methods APACHE Ⅱ score and SIRS score were documented in 140 old patients with sepsis,who were divided into Multiorgan dysfunction syndrome (MODS) group and non MODS group. Results The levels of FT3 and FT4 descended obviously and APACHE Ⅱ score and SIRS score increased remarkably in old patients with severe sepsis(P <0.05 or P <0.01). The level of FT3 and FT4 in death group and MODS group was much lower than that in the survival group (P < 0.05). Conclusion Inflammatory resction and eytokines may effect the hypothalamic- pituitary-thyroid axis. The levels of thyroid hormone can reflect old patients with sepsis condition. The patients with severer conditions may have lower levels of thyroid hormone who will be more likely to died of MODS.

Objective To study the effect of case-based learning(CBL) combined with problem-based learning (PBL) in emergency teaching for foreign students.Methods Totally 33 foreign students were selected in department of emergency,Xuanwu Hospital of Capital University of Medical Sciences and were randomly divided into the observation group (n =17) and control group (n =16).Students in observation group were taught by CBL combined with PBL while those in control group were taught by traditional teaching method.Theory examination,physical examination and Sirm4000 simulated cardiopulmonary resuscitation skill appraisal were given at the end of the internship and achievement differences were compared between 2 groups.Attitude on the teaching method was studied in observation group by questionnaire.SPSS 17.0 software was used for statistical analysis； measurement data were expressed as mean standard deviation ((x) ± s) ； independent sample t-test was applied for comparison between groups； P ＜ 0.05 stands for difference having statistical significances.Results Theory result was (83.5±7.9) in observation group and (76.6 ±8.7) in control group (P=0.012).Cardiopulmonary resuscitation skill score was (84.3 ± 10.1) in observation group and (75.6-± 11.3) in control group (P =0.013).100.0％ students thought that the teaching method could improve the ability of self-learning.Conclusions Teaching method of CBL combined with PBL can stimulate the students' interest in learning and improve students' achievement.

Objective To study teaching method in emergency education of 7-year medical students during clinical practice. Methods 7-year program students during clinical practice were taught by PBL combined with computer simulation teaching and the teaching contents were acute coronary syndrome,upper gastrointestinal bleeding and cardiopulmonary resuscitation. The outcomes of theoretic examination,technique examination and teaching effect investigation were analyzed. Results After clinical practice,students’grades in acute coronary syndrome,upper gastrointestinal bleeding and cardiopulmonary resuscitation were improved obviously(P

Objective To study effect and significance of systemic inflammatory response syndrome(SIRS) score and APACHE Ⅱ score in prognosis in severe patients of emergency department.Methods Data of 114 patients were collected at admission.SIRS score and APACHEⅡ score were calculated in 24 hours.To analyze mortality of patients in different score,to estimate dependability of them.Result With SIRS score increasing,mortality rate increased as well and patients with SIRS score ≥2 or APACHE Ⅱ score ≥25 had significantly higher mortality rate.Conclusions SIRS score is a simple,useful predictor of outcome in patients of emergency department.It can be used in clinical generally.

BACKGROUND: Nanohydroxyapatite reinforced polyamide 66 (n-HA/PA66) prepared using the novel process based on nanohydroxyapatite (n-HA) sol may promote the biocompatibility due to the well distribution of n-HA in the polyamide66 (PA66) matrix and chemical bond at organic-inorganic interface, n-HA/PA66 composite has been proposed as a premising bone repair biomaterial.OBJECTIVE: To investigate the biocompatibility of the novel material both in vitro and in vivo.METHODS: Primarily cultured osteoblasts were co-cultured with n-HA/PA66 and PA66. The cell attachment and morphology were studied using phase contrast light microscope (PCLM) and field-emission scanning electron microscope (FE-SEM).Moreover, n-HA/PA66 pins, with PA66 pins as control, were implanted into the right and left (control group) shinbone shafts of the rabbits, respectively. Materials were harvested at weeks 2, 8 and tissue sections were observed.RESULTS AND CONCLUSION- n-HA/PA66 and PA66 exhibited no cytotoxicity with osteoblasts, while the cellular morphology near the samples was better in the test group than in the control one. Moreover, the cell density attached on n-HA/PA66 was higher than that on PA66 and the cell number were especially different after co-culturing for 3 days (P < 0.01 ). The osteoblasts at the interface between the host bone and n-HA/PA66 also had a higher activity during the earlier period after implantation.Meanwhile the bone formation process was faster and effective in the experimental group. Results suggested that n-HA/PA66 prepared base on the n-HA sol has a better biocompatibility as compared with PA66.

Objective To investigate the role of protein phosphorylation in Pseudomonas aeruginosa (P.aeruginosa) strains in response to stress triggered by mouse macrophages.Methods The strong cation exchange-immobilized metal affinity chromatography (SCX-IMAC) was performed to enrich phosphopeptides.The nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) was carried out to identify and analyze phosphoproteome.Results Fourteen phosphopeptides from twelve proteins were identified within thirty-one phosphorylation sites on serine,threonine and tyrosine residues.Fifty percent of these phosphorylated proteins were membrane proteins,indicating that their phosphorylation modification was more critical for bacteria in response to the stress.In terms of biological process of Gene Ontology,these identified proteins were involved in stress response,iron transport,anaerobic respiration,response to hydrogen peroxide and signal transduction by phosphorylation,etc.Conclusion These phosphorylated proteins in P.aeruginosa strains are necessary for signal transduction and their response to harsh environment within the macrophages,such as iron limitation,hypoxia and oxidative stress.This study provides evidence for further investigation on virulence and pathogenesis of P.aeruginosa.

Objective To study the influence of different kinds of disinfection methods on antibacterial activity of TiO2 nanotubes. Methods TiO2 nanotubes were fabricated with 0.5%wt HF on pure titanium to acid-etching combined with an? odization as experimental group. The pure Ti was control group. They were disinfected by three kinds of disinfection meth?ods, which were alcohol immersion, autoclaving and UV irradiation. The bacterial adhesion ability was evaluated by observ?ing the morphology of samples, measuring the roughness and contracting angle. The film contact method was used to evaluate the antibacterial activity of samples with Staphylococcus aureus (Sa) after different infection treatment. The bacterial morphol?ogy was observed by field emission scanning electron microscope (FE-SEM). Results The treatment group has higher sur?face roughness. The bacterial adhesion ability of experimental group was stronger compared with that of control group. The contracting angle of samples exposed to UV irradiation was smaller than that of alcohol immersion and autoclaving treatment group. The viable count and FE-SEM showed that there were more bacteria and more complete morphology on the sample surface after alcohol immersion. The viable count was less and bacterial morphology suffered some damage on the sample sur?face after high pressure steam sterilization. The least viable count and the most complete destruction of bacterial morphology were found after ultraviolet (UV) germicidal irradiation. Conclusion UV irradiation shows the best antibacterial activity, the second is autoclaving and the worst is alcohol immersion.