1.The evaluation of taking the opportunity of family planning B ultrasound check of married women in childbearing age to carry out anti AIDS education
Hairong GUO ; Haijun WANG ; Changxi SUN
Journal of Chinese Physician 2015;(z1):13-16
Objective To explore the married women of childbearing age education on HIV/AIDS intervention method, curb the spread of HIV.Methods Use family planning check B to exceed three op-portunity, for married women of childbearing age to carry out the publicity and consultation, and design a u-nified questionnaire, respectively in the survey of 500 were statistically analyzed before and after the inter-vention, effect evaluation in description epidemiological methods.Results Before and after the interven-tion, the AIDS knowledge awareness rate of married women of childbearing age was 62.75%(2183/3479) , 97.36%(3360/3451),attitudes to AIDS positive response rate was 65.19%(324/497), 95.94%(473/493), middle school students knowledge of AIDS awareness rate was 90.48% (5130/5670), 96.99%(5110/4956 ) , the villagers'awareness rate of AIDS related knowledge was 69.61% ( 2183/3136 ) , 97.36% (3360/3451) .After the intervention, the AIDS knowledge awareness and attitude to HIV positive answer rate of married women of childbearing age were significantly improved,middle school students, vil-lagers AIDS awareness rate were significantly higher than those before intervention.Conclusions The use of B-three examinations opportunities for married women in AIDS prevention knowledge of educational inter-vention improves the life skills of women of childbearing age to prevent AIDS, and to take advantage of the core and the influence of women of childbearing age in the family will spread AIDS prevention knowledge, radiation to family members and the community to improve AIDS prevention capacity of the whole society, to curb the AIDS epidemic.
2.Associations of SUMO4 polymorphisms with tacrolimus concentrations in Chinese renal transplant recipients.
Xiaoman LIU ; Jiali LI ; Hongyang WANG ; Qian FU ; Jun LI ; Shu LIU ; Pingping SUN ; Changxi WANG ; Min HUANG
Acta Pharmaceutica Sinica 2015;50(2):180-4
The study aims to investigate the associations of SUMO4 polymorphisms with tacrolimus concentrations in Chinese renal transplant recipients. Blood samples and clinical data were collected from 132 renal transplant recipients with tacrolimus treatment. CYP3A5*3 genotypes were detected by PCR-RFLP, and SUMO4 (rs237024, rs237025) genotypes were detected by Sequenom® MassARRAY system. SUMO4 rs237024 and rs237025 genotypes were in complete linkage disequilibrium (D' = 1). The dose-adjusted concentration of tacrolimus in SUMO4 rs237024A-rs237025A (GA-GA +AA-AA) carriers was considerably higher than that in GG-GG carriers (P < 0.05). After stratification by CYP3A5*3 genotypes, SUMO4 rs237024A-rs237025A carriers (GA-GA+AA-AA) had a higher dose-adjusted tacrolimus concentration than that in GG carriers in CYP3A5 expresser (P < 0.05). The results illustrated that SUMO4 rs237024 and rs237025 polymorphisms were associated with tacrolimus concentrations, and the test of these genotypes may be useful for individualized medicine of tacrolimus.
3.Investigation report on the outbreak of diarrhea in the second middle school of Zhushan county in Hubei Province
China Modern Doctor 2015;(19):126-129
Objective To master the characteristics of the outbreak of diarrhea,so as to provide evidences for formulat-ing the measures of control and prevention. Methods Epidemiological data and laboratory data of registered patients were investigated,and descriptive epidemiological method was applied to statistically analyze the data. Results A total of 235 patients were infected, and the morbidity rate was 7.66%. Morbidity rate in cooks was significantly higher than that in students, teaching staff and family members, males higher than females, and on-campus students higher than off-campus students; the water plant supplied drinking water not conforming to the standard of hygiene,and the cooks were not detected timely and transferred from the current position after being infected with bacillary dysentery and di-arrhea. The bacteria was transmitted via water and food,combined with the transmission via intimate contact and carri-ers by flies. Conclusion Management on the hygiene of food and drinking water in the school should be strengthened,environment disinfection and insecticide should be carried out,and the population in epidemic focus of diarrhea should be orally given norfloxacin for prevention; the supervising mechanism of infectious diseases in the school is estab-lished,potential safety hazard is timely dealt with,and water-borne communicable diseases can be prevented.
4.Impact of CYP3 A5 genetic polymorphism on modified releasing tacrolimus pharmacokinetics in Chinese renal transplant recipients
Pan CHEN ; Qian FU ; Jingjie LI ; Pingping SUN ; Rongrong DENG ; Jun LI ; Xiaoman LIU ; Hongyang WANG ; Min HUANG ; Xiao CHEN ; Changxi WANG
Chinese Pharmacological Bulletin 2016;32(11):1592-1595
Aim To investigate the impact of CYP3 A5 genetic polymorphism on modified releasing tacrolimus pharmacokinetics in Chinese stable renal transplant re-cipients. Methods Pharmacokinetics of once daily-ta-crolimus( tac-q. d. ) and twice daily-tacrolimus( tac-b. i. d. ) were determined by CLIA, CYP3A5 genotype was measured by PCR-RFLP. Each 10 patients receiv-ing tac-q. d. and tac-b. i. d. respectively were en-rolled, and each 5 patients receiving tac-q. d. were matched to poor metabolizer ( PM ) and extensive me-tabolizer ( EM ) group respectively according to CYP3A5 genotypes. Results AUC0~24 h for tac-q. d. was 1. 78 folds higher than AUC0~12 h for tac-b. i. d. , and dose-adjusted C0 was 40% lower for tac-q. d. than for tac-b. i. d. There were no significant differences for other parameters between the two groups; Cmax, AUC0~24 h and C0 were 1. 75, 1. 96 and 2. 49 folds higher for PM than for EM, and dose-adjusted Cmax, AUC0~24 h and C0 were 1. 80, 2. 34 and 2. 64 folds higher for PM than for EM. There were good correla-tions between AUC0~24 h and C0 for tac-q. d. Conclu-sion Conversion from tac-b. i. d. to tac-q. d. results in requirement of increased tacrolimus dose and detec-tion of CYP3A5 genotype, which is necessary for ensu-ring C0 in the range of therapeutic window.
5.Granulocyte colony-stimulating factor combined with high-intensity intermittent exercise preconditioning improved cardiac remodeling in rats with acute myocardial infarction
Yuma SUN ; Wenchao MA ; Changxi FU
Chinese Journal of Tissue Engineering Research 2024;28(31):4987-4994
BACKGROUND:Exercise training is an important non-drug rehabilitation method for many heart diseases,and it can also enhance the heart's tolerance to adverse stressors(such as myocardial ischemia),that is,exercise preconditioning.Granulocyte colony-stimulating factor can effectively mobilize stem cell homing and differentiation and promote the repair of damaged myocardium.However,the effect of the combination of the two treatments is not yet clear. OBJECTIVE:To explore the effect of granulocyte colony-stimulating factor supplementation combined with high-intensity intermittent exercise preconditioning on cardiac remodeling in rats with acute myocardial infarction and investigate its possible mechanism. METHODS:Totally 58 male Wistar rats were divided into sham group(n=10),model group(n=12),model drug group(n=12),model exercise group(n=12)and model combined treatment group(n=12).The myocardial infarction rat model was made by coronary artery ligation.The model exercise group and the model combined treatment group were pretreated with 8 weeks of high-intensity intermittent exercise on an electric treadmill before modeling.The model drug group and the model combined treatment group were subcutaneously injected with human recombinant granulocyte colony-stimulating factor 3 hours after modeling for 5 days(10 μg/kg per day).At 8 weeks after administration,echocardiography was used to detect heart structure and function;heart was stained with 2,3,5-triphenyltetrazolium chloride and Masson staining to obtain myocardial infarct area and collagen volume fraction,respectively.Vessel density and cell apoptosis rate were detected by immunofluorescence.Real-time fluorescent quantitative PCR was utilized to detect the mRNA expression of embryonic genes(brain natriuretic peptide,β-myosin heavy chain)and myocardial contraction regulatory factors(α-myosin heavy chain,sarcoplasmic reticulum Ca2+-ATPase).Western blot assay was used to detect the protein expression of cardiac stromal cell-derived factor 1,CXC chemokine receptor protein 4,Janus kinase 2(JAK2),signal transducer and activator of transcription 3(STAT3),Bcl2,Bax,and cleaved caspase-3. RESULTS AND CONCLUSION:(1)Compared with sham group,myocardial infarct size,collagen volume fraction,and apoptosis rate increased(P<0.05);vessel density,left ventricular fractional shortening,and left ventricular ejection fraction decreased(P<0.05);brain natriuretic peptide and β-myosin heavy chain mRNA increased(P<0.05),α-myosin heavy chain and sarcoplasmic reticulum Ca2+-ATPase mRNA and α-myosin heavy chain/β-myosin heavy chain ratio decreased(P<0.05);stromal cell-derived factor 1,CXC chemokine receptor protein 4,Bax,cleaved caspase-3 protein expression increased(P<0.05);p-JAK2,p-STAT3,and Bcl-2 protein expression decreased(P<0.05)in the model group.(2)Compared with the model group,the above indexes in the model drug and model exercise groups were significantly improved(P<0.05).Compared with the model drug and model exercise groups,the above parameters were further ameliorated(P<0.05)in the model combined treatment group.(3)The results showed that supplementation of granulocyte colony-stimulating factor or high-intensity intermittent exercise preconditioning alone can improve cardiac remodeling in rats with acute myocardial infarction,and the combined therapy has a better effect,which may be related to the induction of stem cell homing and the activation of JAK2/STAT3 signaling pathway to inhibit cardiomyocyte apoptosis.
6.Application of epidural block combined with general anesthesia in upper abdominal and thoracic tumor surgery study
Qingshan SUN ; Changxi SHAO ; Zhengyan ZHANG ; Dianhong WU ; Chunli WEI
China Modern Doctor 2015;(15):105-107
Objective To compare the effect of epidural anesthesia combined with general anesthesia and general anesthesia in upper abdominal and thoracic tumor surgery anesthesia. Methods A total of 80 cases of ASAⅠ-IIelective surgery with the abdomen and chest patients were selected from July 2013 to December 2014 in our hospital, and divided into two groups, each of 40 cases, group A was treated with epidural block joint general anesthesia, group B was treated with general anesthesia. The amount of the anesthesia drug, anesthesia recovery and hemodynamic changes at different times of two groups were compared. Results Propofol, fentanyl and cis-atracurium dosage of group A were significantly reduced than group B, spontaneous breathing surgery recovery time, extubation time and fully awake time were significantly shorter, the occurrence of emergence agitation pain was significantly lower, the differences were statistically significant(P<0.05). HR in group B on T2 was significantly faster than group A, HR, SBP, DBP on T3 and T4 were significantly higher than that in group A, the differences were statistically significant (P<0.05). Conclusion Compare with general anesthesia, anesthesia of intraoperative hemodynamic stability, can suppress the stress response to surgery, recovery after extubation time is shorter than the general anesthesia group, perioperative application is more security and stability.
7. Clinicaland prognostic features of lipoprotein glomerulopathy in renal allografts
Jin ZHANG ; Xutao CHEN ; Gang HUANG ; Jiang QIU ; Guodong CHEN ; Lizhong CHEN ; Jiguang FEI ; Changxi WANG
Chinese Journal of Organ Transplantation 2019;40(10):620-623
Objective:
To explore the clinical and prognostic features of lipoprotein glomerulopathy (LPG) in renal allografts.
Methods:
Retrospective analysis was performed for two case of LPG in renal allografts. The onset time was 6 and 9 years after living transplantation respectively. Initial symptoms included proteinuria and hypoproteinemia. Color Doppler ultrasound showed an enlarged graft size and greater parenchymal echogenicity. One patient had hyperlipemia and elevated apolipoprotein E (ApoE). Methylprednisolone pulse was offered with an early control of hyperlipidaemia and proteinuria by fenofibrate and angiotensin-converting enzyme inhibitors (ACEIs). Yet it had no effect on graft function. The definite diagnosis was made by graft biopsy. Pathological examination indicated non-homogeneous lipid deposition in glomerular capillary, glomerular sclerosis, mesangial hypercellularity and tubular atrophy.
Results:
During a follow-up period of 8 and 10 years post-transplantation, two cases eventually lost their grafts within 2 and 1 year after biopsy respectively. With long-term dietary control and drug therapy, regular dialysis continued and both awaited a second transplantation.
Conclusions
LPG is generally steroid-resistant and refractory in renal allografts. And routine biopsy is recommended for patients with a high risk of occurrence. Early controls of hyperlipemia and hypoproteinemia and other risk factors should be also properly managed.
8.Efficacy and safety of single high-dose versus multiple low-dose ATG-Fresenius induction in de novo renal transplantation
Liping CHEN ; Chunbai MO ; Jun TIAN ; Guanghui PAN ; Changxi WANG ; Jianghua CHEN ; Tao LIN ; Xiaodong ZHANG ; Yaowen FU ; Long LIU ; Zhishui CHEN ; Aimin ZHANG ; Minzhuan LIN ; Xuyong SUN ; Jinsong CHEN ; Hang LIU ; Shaoling ZHENG ; Tongyu ZHU ; Qingshan QU ; Bingyi SHI
Chinese Journal of Organ Transplantation 2017;38(11):665-670
Objective To evaluate the efficacy and safety of single bolus high dose (SD group) ATG-Fresenius induction therapy in kidney transplantation vs.multiple low dose (MD group) administration.Methods A multiple center,prospective,randomized and controlled clinical study was performed on 280 de novo renal transplant recipients from 19 centers.Patients were randomized into 2 groups as follows:SD group,a single high dose (7-9 mg/kg) of ATG-F infused as an induction agent before the vessel anastomoses;MD group,2 mg/kg of ATG-F daily administrated in postoperative 4 days.All the patients accepted maintenance immunosuppressive protocol including tacrolimus,mycophenolate and prednisone.Patients were assessed and data were collected at regular schedule clinic visits on the day 1,3,7,14,30,90,180,270 and 365.The primary end point of efficacy was therapeutic failure rate [the number of death,grafts loss and acute rejection (AR)].The event first occurred should be used in the classification of patients.The non-inferiority evaluation of the two treatment regimens was done based on treatment failure rate.The secondary end points of efficacy were the incidence of AR,delayed graft function (DGF),1-year survival rate of patients and grafts,and serum creatinine at each visiting point.The indicators for safety evaluation included hemotologic variation and incidence of adverse events.Results The therapeutic failure rate in SD group was non-inferior to the MD group (17.24% vs.23.08%).AR was the major cause of therapeutic failure and there was similar incidence of AR between SD gronp and MD group (12.07% vs.21.37%).There was no significant difference in the incidence of DGF between SD group and MD group (12.07% vs.6.84%,P =0.1721).The 1-year patient's survival rate and 1-year graft survival rate in SD group and MD group showed no significant difference (96.55% vs.98.29%,P =0.6714;94.83% vs 98.29%,P =0.2750).The serum creatinine level showed no significant differences between two groups at each visit point.There was also no significant difference in total incidence of adverse events between the two groups.In addition,there was also no statistically significant difference in the incidence of concerned and drug-related adverse events between the two groups,including infection,hemotologic abnormality,liver or renal dysfunction,gastrointestinal disorder,etc.After ATG--F administration,peripheral blood lymphocytes in the SD and the MD group immediately decreased but nearly restored to the normal level on the postoperative day 30 and 90 respectively.No severe granulocytopenia,erythropenia or thrombocytopenia occurred in both two groups.Conclusion The efficacy and safety of single high dose of ATG-F induction are non-inferior to multiple low dose ATG-F induction,moreover,single high dose of ATG-F induction is administered more conveniently and economically.
9.Conversion from mycophenolic acid to mizoribine in renal transplant recipients with gastrointestinal tract symptoms: a prospective observational multi-center study
Bingyi SHI ; Jianxin QIU ; Weiguo SUI ; Jun TIAN ; Youhua ZHU ; Chunbo MO ; Changxi WANG ; Yingzi MING ; Zhishui CHEN ; Yaowen FU ; Zheng CHEN ; Longkai PENG ; Zhilin HU ; Tao LIN ; Xuyong SUN ; Hang LIU ; Ruiming RONG ; Ye TIAN ; Wujun XUE ; Ming ZHAO ; Guiwen FENG ; Ronghua CAO ; Decheng DENG ; Minzhuan LIN ; Xiaodong ZHANG
Chinese Journal of Organ Transplantation 2017;38(12):708-713
Objective To evaluate the effect of conversion from mycophenolic acid (MPA) to mizoribine (MZR) in renal transplant recipients with gastrointestinal tract (GI) symptoms.Methods A total of 355 renal transplant recipients with GI symptoms caused by MPA administration were enrolled from April 2015 to March 2017 in 25 different renal transplant centers in China.The symptomatic improvement of GI before (baseline) and after conversion to MZR (1,2,4 weeks) was assessed by each item of GI symptoms indication.In addition,the efficacy and safety of the conversion therapy during 12 months were determined.Results Patients showed improvement in GI symptoms including diarrhea,abdominal pain,abdominal distention and stomachache after conversion to MZR 1,2,4 weeks (P<0.05).In patients with different severity of diarrhea,conversion to MZR therapy significantly improved diarrhea (P<0.05).During 12 months,no patient experienced clinical immune rejection.We did not observe any infections,leucopenia and other serious side effects.Conclusion MZR could markedly improve GI symptoms caused by MPA administration in renal transplant recipients.
10.Current status of pediatric kidney transplantation in China: data analysis of Chinese Scientific Registry of Kidney Transplantation.
Longshan LIU ; Huanxi ZHANG ; Qian FU ; Liping CHEN ; Chuanhou SUN ; Yunyi XIONG ; Bingyi SHI ; Changxi WANG
Chinese Medical Journal 2014;127(3):506-510
BACKGROUNDKidney transplantation (KTx) is the primary therapy for children with renal failure. Unlike KTx in adult patients, it is commonly agreed that pediatric KTx in China is far behind that of America. There has been no systematic analysis of Chinese pediatric KTx reported. This study aimed to demonstrate the current status of pediatric KTx in China.
METHODSRegistry data of pediatric KTx (1983-2012) from Chinese Scientific Registry of Kidney Transplantation (CSRKT) were retrospectively analyzed.
RESULTSThere were 851 pediatric KTx from 102 transplant units. The recipients were (15.4±2.5) years of age, 93.9% of who were over 10 years old. Chronic glomerulonephritis and pyelonephritis accounted for 75.6% of recognized primary diseases. Allografts were from deceased donors (72.2%) or living donation (27.7%). The patient survival for 1, 3, 5, and 10 years was 96.9%, 94.2%, 92.3%, and 92.3% and the graft survival was 94.6%, 91.4%, 86.3%, and 79.2%, respectively. The majority of post-transplant complications were acute rejection and infections. Annual transplant reached the peak in 2008 (n = 114), and decreased sharply in 2006 (n = 41) and 2010 (n = 57). The percentage of pediatric KTx in total KTx was highest in 2007 (1.95%) and decreased to trough level in 2010 (1.0%). Living donation increased by 32.5-folds from 2004 to 2008 and then decreased by 86.6% till 2010. The percentage of living donation in pediatric or total KTx dynamically changed in a similar manner, while living donation ratio in pediatric KTx was much higher.
CONCLUSIONSKidney transplant can provide long-term benefits to pediatric recipients. Rejection and infections are worthy of concern during follow-up. Pediatric kidney transplant in China is very much lagging behind that in developed countries. Living donation played an important role in its development in the past decades. New strategies for implementation are encouraged to increase the priority of uremic children in organ allocation so as to promote its progress in China.
Adolescent ; China ; Female ; Humans ; Kidney Transplantation ; statistics & numerical data ; Male ; Retrospective Studies