BACKGROUND: Transforming growth factor beta (TGF-β) has an important role in tendon healing and adhesion formation.Inhibiting TGF-β and its receptor expression may prevent adhesions after tendon open.OBJECTIVE: To study the effects of mannose-6-phosphate, a natural inhibitor of TGF-β, on TGF-β and its receptor production in tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes of rabbit flexor toes.METHODS: Tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes were isolated from rabbit flexor tendon and cultured separately. All these cells were divided into 2 groups at random, experiment group supplemented with mannose-6-phosphate and control group without mannose-6-phosphate. The expression of TGF-β and TGF-β receptor was quantified with enzyme-linked immunosorbent assay. The expression of TGF-β1 was also assessed with in situ hybridization and immunohistochemistry.RESULTS AND CONCLUSION: The expression of TGF-β and TGF-β receptor in experiment group was significantly lower than that in control group (P ＜ 0.05). In experimental group, the positive expression of TGF-β1 mRNA and the expression level of intracellular TGF-β1 mRNA in all tendon cells demonstrated significantly lower than those in the control group (P ＜ 0.05).Immunohistochemical staining showed expression of TGF-β1 were significantly lower in all three types of tendon cell cultured with mannose-6-phosphate.

Objective To investigate the effect of endothelial progenitor cells (EPCs) on the growth of C6 glioma cells in vitro.Methods EPCs were obtained from the spleen of healthy SD rats with density gradient centrifugation and adherence screening.The obtained EPCs were identified through morphologic characteristics,specificity to DiI-acLDL uptaking and Lectin binding,and positive expressions of CD34 and CD31 by immunofluorescence assay.The EPCs-conditional medium was added into the convention medium of C6 glioma cells to assess its effect on the proliferation of glioma cells.After cultured for 36 or 48 h with the EPCs-conditional medium or conventional medium (control),MTT assay was employed to measure the cell proliferation and flow cytometry was used to detect the cell cycle.Results In 10 d after culture,the attached cells presented a "line-like" structure,and the adherent cells were double positive to DiI-acLDL uptaking and FITC-UEA-1 binding by direct flourescence staining under a laser scanning confocal microscope.Those cells were differentiated EPCs,and expressed wholly expressed CD34 and CD31.MTT assay showed that the OD value of each group at both the 2 time points were increased with the increasing of EPCs content in conditioned medium.The OD value of the group containing 50% of EPCs conditional medium of 36 h(2.018?0.220) and 48 h (2.388?0.448) was markedly higher than those of the control group (1.163?0.103,1.106?0.174) with significant difference (P

Objective To assess the value of clinical applications of whole-body diffusion-weighted imaging (WB-DWI) in diagnosing patients with malignant tumors compared with positron emission tomography (PET). Methods A total of 22 patients with highly suspected malignant tumors underwent WB-DWI after PET. The differences between the two imaging methods were compared in displaying lesions, and the correlation between ADC and SUV value was analyzed. Results More lesions were showed with WB-DWI than PET. There was no significant difference between the two methods in detecting the lesions of lung, mediastinal septum or abdomen (P>0.05), but more lesions in skeleton were showed with WB-DWI (P<0.05). No significant correlation between ADC and SUV value was found. Conclusion Compared with PET, WB-DWI can detect more tumor lesions. The sensitivity of WB-DWI in detecting metastatic tumors of bone is higher than that of PET.