To study the secretion of epidermic growth factor (EGF) in tears from patients with herpes simplex virus keratitis (HSVK), radio-immunoprecipitation was used to detect the value of EGF in human reflex tears from 40 cases as control group (group A), 49 cases of acute HSVK (group B) and 31 cases of convalescence HSVK (group C). Results showed that the value of EGF in reflex tears from the patients in the group B was obviously increased as compared with that in the group A and group C (P<0.001). The value of EGF in tears in the group C was higher than in group A (P<0.05). It was concluded that the value of EGF in reflex tears from HSVK patients was higher than that from normal people.

DNA methylation is the best studied of the epigenetic changes that influence the gene expression,and changes in genomic methylation patterns have been observed in many cancer cell lines.Nutrients as methyl donors play essential roles in DNA methylation and may modify cancer risk.This review,with an emphasis on the relationship between nutrition,DNA methylation and cancer,firstly defines methylation with a description of its correlated mechanism,then approaches the molecular mechanism of cancer initiation and development at the methylation level,and lastly summarizes the mechanism underlying the effect of methylation-related nutrients on the process of cancer initiation and development at the molecular level.It also offers some suggestions on cancer prevention at the group nutrition level.

Objective To study the role of gasless laparoscopic techniques in the treatment of abdominal trauma. Methods A retrospective analysis was made on clinical data of 52 cases of abdominal trauma considered unfit for pneumoperitoneum treated by gasless laparoscopic surgery. Results Prompt diagnosis and treatment were achieved in 43 cases. A conversion to laparoscopic exploration under pneumoperitoneum was required in 5 cases. The remaining 4 cases of severe injuries of the spleen and the bowel were cured by a conversion to open surgery after the determination of the incision site under gasless laparoscope. There were no fatal cases in the study. Conclusions Use of gasless laparoscopic techniques for the diagnosis, treatment and determination of incision site in patients with abdominal trauma considered unfit for pneumoperitoneum is feasible. It is a necessarily additional alternative to traditional laparoscopic surgery.

Objective To study the relationship between occupational stress and metal health of radiation workers.Methods Totally 220 radiation workers were investigated by Chinese perceived stress scales questionnaire,Chinese health questionnaire-12,as well as questionnaire related to radiology.Results The stress score of radiation worker with senior title was 18.18 ± 6.12,which was the lowest of all 220 participants.There was positive relationship between stress score and health score (r =0.484).19.1％ of the workers sometimes felt stressed due to the danger of work.Conclusions There are some significantly correlation between occupational stress and metal disorder.More attention should be paid on the occupational stress.Positive measures such as decompression and psychological intervention will be helpful to protect physical and mental health of radiation workers.

Objective To explore a new microsurgical method for reconstruction of finger pulp defect.Methods From May 2008 to May 2009,10 male patients with finger pulp defect were treated in our hospital,aged from 18 to 38 years (average,26 years).The index finger was involved in 6 patients,the middle finger in 3 patients and the ring finger in 1 patient.All finger pulp defects were caused by machine injury.The defect sizes ranged from 1.5 cm×2.0 cm to 2.0 cm×3.0 cm.Six patients suffered from single skin defect,and 4 patinets suffered from skin defect combined with nail bed laceration and distal phalangeal fractures.All patients were performed emergency operations.The defects were reconstructed by using arterialized venous flap with microsurgical suture of the dorsal branch of the proper digital nerve.The fractures were fixed by Kirschner wires.The donor area was covered with skin grafts.Results All flaps survived completely.The fractures healed 8 to 10 weeks postoperatively.All patients were followed up for 4 to 6 months,all flaps presented satisfactory appearance,normal texture,with no pigmentation at the last followup.The static 2-point discrimination of the flaps ranged from 8 to 12mm.All injured fingers obtained good recovery of flexion and extension of the distal interphalangeal joints.The nails of the fingers with laceration of nail bed grew smoothly.The nail bed with laceration grew smoothly,and some new nails could be seen.The skin grafts applied to the donor area survived completely.Conclusion The arterialized venous flap with suture of the dorsal branch of the proper digital nerve is a good method for reconstruction of finger pulp defect,which had the following advantages:slight donor injury,low anesthesia risk,simple operative technique,and satisfactory postoperative function and appearance.

Objective To study the therapeutic effects of extracorporeal shock wave therapy (ESWT) on temporomandibular joint disorders (TMDs).Methods Sixty patients with a temporomandibular joint disorder were randomly divided into an ESWT group and a control group,each of 30.Both groups were given routine rehabilitation training,but the treatment group additionally received ESWT for 2 weeks.Fricton's index was used to evaluate temporomandibular joint function.The subjects used a visual analogue scale (VAS) to evaluate their pain levels.The SF-36 scale was applied to assess their life quality.Results After two weeks of treatment,the average assessment score and VAS score had decreased significantly in both groups.After treatment the average scores for mandibular movement,muscle palpation,joint noise and joint palpation,and the palpation,dysfunction and craniomandibular indexes were all significantly lower in the ESWT group than in the control group.Significant differences were also found in the average VAS scores and on the social functioning domain of the SF-36 between the ESWT group and the control group.Conclusion ESWT combined with rehabilitation training can significantly relieve the symptoms and pain of TMDs.

Objective To observe the effect of Duanteng Yimu decoction (DYD) on bone turnover markers of C-terminal telopeptide of type Ⅰ collagen (CTX-Ⅰ) and N-terminal propeptide of type Ⅰ procollagen ( PⅠNP) of adjuvant-induced arthritis (AA) rats. Methods Seventy-two SD rats were divided into 9 groups, and complete Freund's adjuvant was injected into the rats to establish adjuvant-induced arthritis rat model. After medication for 36 days, the pedal swelling, the serum levels of CTX-Ⅰand PⅠNP, and ankle X-ray photographic scores were observed. Results The pedal swelling degrees and ankle X-ray scores in medication groups were lower than those in the model group ( P<0.05). The PⅠNP level had no statistically significance in various groups ( P>0.05). The CTX-Ⅰlevel in low-dose DYD group was decreased (P<0.05). Conclusion Caulis Celastri has two-way effects on CTX-Ⅰlevel of female AA rats, and its side effects can be reduced by Radix Dipsaci and Herba Leonuri, but their antagonism effect will disappear with the increase of the dose of Caulis Celastri.

Objective To observe the bioactivity of recombinant human scFv antibody against NH-lipopolysaccharide binding protein in vitro and in vivo. Methods KM mice, weighing 20-25 g, were burnt on the back to the third degree covering 20% total body surface area. The mice in only burn group were intraperitoneally injected with 1 ?g/g LPS and those in scFv-treated group with 1 ?g/g LPS and 0.5 ?g/g scFv after burn. The mice without any treatment served as controls. All mice were sacrificed in 1, 3, 6, 12, 24 h after all treatment and the whole blood, the tissues of liver, lung and kidney were collected. The serum concentration of TNF-?, IL-6 in burnt mice with endotoxemia was detected by ELISA and the pathological changes of liver, lung and kidney tissues were observed by light microscope. The inhibition of scFv antibody of different concentrations against NH-lipopolysaccharide binding protein on FITC-LPS binding with U937 cells was assayed by FCM. Results The histopathological changes of liver, lung and kidney in mice of scFv-treated group revealed that less inflammatory cell infiltration, less degenerated cells, weaker congestion in tissue as compared with control and only burnt mice. The serum concentration of TNF-? in only burnt mice increased significantly than that of the controls (P

Objective:To investigate the effects of probiotics on the stabilization of blood glucose and its mechanism in children with severe infectious pneumonia.Methods:Eighty children with severe infectious pneumonia admitted to PICU were enrolled in the clinical trial prospectively.They were divided into two groups by random.Forty patients given probiotics were regarded as treatment group,the others not given probiotics were considered to be controlled group,in addition to the regular therapy.Blood glucose,plasma H2 S,D-lactate and acetic acid,propionic acid and butyric acid concentrations in stools were measured respectively at the beginning of the treatment and on day 8 after the treatment.At the same time the changes of blood glucose,fluctuation of them and prognosis were examined.Results:There was no significance in all the parameters of the two groups at the beginning of the treatment (P ＞ 0.05).The levels of plasma H2S and acetic acid,butyric acid in the stools of the probiotics group was (24.6 ± 7.4) μmol/L,(32.8 ± 8.6) μmol/g,(15.2 ± 5.2) μmoL/g on the day 8 respectively;but the concentration of these parameters in the control group was (20.9 ± 7.4) μ mol/L,(28.6 ± 7.9) μmol/g,(12.9 ± 5.0) μmoL/g.Meanwhile the level of D-lactate in patients given probiotics (1.25 ± 0.39) μg/ ml was significantly lower than that the control group (1.45 ± 0.4) μg/ml.The blood glucose (6.1 ± 2.7) mmol/L was lower than than in the other group (7.4 ± 2.7) mmol/L on day 8.And a higher recov ery rate was found in probiotic group.Furthermore,blood glucose and H2S,acetic acid levels expressed negative correlation at 8 day;but positive correlations were found between blood glucose and D-lactate (P ＜ 0.05).Conclusion:Application of probiotics to children with severe infectious pneumonia can moderate the structure of enterobacteria,reinforce the intestinal barrier and elevate the levels of plasma H2S and had positive effects on the stabilization of blood glucose and prognosis.

Objective To use Monte Carlo method to build a shielding calculation model for the proton and heavy ion treatment room,and to provide a reliable calculation method for shielding design.Methods A Monte Carlo-based FLUKA code was adopted to build the shielding calculation model for the proton and heavy ion treatment room,and to simulate the radiation field distribution in the proton and heavy ion treatment room.The calculation model was verified through the radiation detection around the proton and heavy ions treatment room.Results The FLUKA code-based simulation results were consistent with the radiation detection.Conclusions The shielding calculation model based on FLUKA code can simulate the radiation field from proton and heavy ions.Among the secondary particles,secondary neutrons are the dominant component and the main concern of accelerator shielding design.In shielding calculation,the emphasis should be put on both beam intensity and energy.