Objective To describe the clinical characteristics,outcome and prognostic factors of primary intraspinal tumor treated with postoperative radiotherapy.Methods Thirty-three patients diagnosed as suffering from intraspinal tumor treated with postoperative radiotherapy were analyzed.There were eighteen male and fifteen female,with a median age of 37 years(range 7-64 years).Two patients were treated with grossly total resection,25 subtotal resection,2 biopsy only.These patients were treated either with(~(60)Co)(n =18,before 1998) or 6?MV-X ray(n =15,after 1998) to the total dose of 45-68?Gy.The overall response and survival rates were calculated by Kaplan-Meier method and the difference between groups was tested by the log-rank method.The prognostic factors related to the characteristics of patient,tumor,and treatment were determined.Results The overall response,five-year and disease-free survival rate was 81.3%,59.4% and 56.3%,respectively.By multivariate analysis,the histological type(P= 0.039) and gender(P= 0.049) had independent impact on the 5-year survival rate.Ependymoma had a better prognosis compared to other histological subsets and female gained more survival advantage over male.There was no close relationship between survival and other factors,such as age,extent of surgery,initial site,irradiation dose,or the interval between surgery and radiotherapy.Conclusions Postoperative radiotherapy is safe and effective for primary intraspinal tumor.The histological type and gender are important prognostic factors of survival in primary intraspinal tumors.

Objective To investigate the mutation status of epidermal growth factor receptor ( EGFR) and KRAS gene in patients with non-small cell lung cancer ( NSCLC) in Xuanwei, Yunnan and to correlate the mutation status with clinicopathologic features.Methods Mutation status of exons 18, 19, 20 and 21 of EGFR, and codons 12, 13 of KRAS in 63 cases of NSCLC were analyzed by gene sequencing and ARMS-Taqman probe method.Correlation with patients′clinicopathological characteristics was performed. Results EGFR and KRAS mutations were present in 55.6% (35/63) and 6.3% (4/63), respectively. EGFR gene mutations were present, including exon 18 G719X in 14.3% (5/35), exon 19 in 14.3%(5/35), exon 20 S768I and T790M in 20.0% (7/35), exon 21 L858R in 31.4% (11/35), exon 18 G719X and exon 20 S768I double mutation in 17.1%(6/35), and exon 20 T790M and exon 21 L858R double mutation in 2.9%(1/35).KRAS mutations were seen in codon 12 in 3 of 4 cases, and codon 13 in 1 of 4 cases.EGFR mutations were mutually exclusive with KRAS mutations.According to statistic analysis, EGFR mutations were associated with the histological types of NSCLC ( P<0.05 ) , but without correlation with patient′s gender, age, smoking status and lymph node metastasis ( P >0.05 ) .KRAS mutations in NSCLC had no correlation with the clinical pathologic characteristics of the patients.Conclusions A higher frequency of EGFR exon 18 G719X and 20 exon S768I mutations are found in the patients in Xuanwei, Yunnan.EGFR mutations are associated with histologic types of NSCLC, but without correlation with patient′s gender, age, smoking status and lymph node metastasis.KRAS mutation in NSCLC has no correlation with the clinicopathologic characteristics of the patients.