To evaluate the effect of vitamin E on GPT, GOT and LDH activity of cold exposed rats, young male rats (70-100g body weight) were randomly divided into Ⅰ-Ⅲ groups. All the animals were fed on basic ration. The group I was given vitamin E 5mg (5mg/ml) intraperitoneally once every other day for 10 days. After the vitamin E treatment, group Ⅰ and Ⅲ were kept in a cold chamber (-1?1℃) and group Ⅱ at room temperature (20?1℃) for 48 hours. At the end of experimental period, serum GPT, GOT and LDH activity of all animals were analyzed. According to the data, the GPT, GOT and LDH activity were increased significantly in group I than in groups Ⅰ and Ⅱ. These results suggested that the vitamin E may have the efficiency of maintaining the structural integrity of the cells of cold exposed animals.

To evaluate the effect of Vitamin E on mitoc bond rial function of cold exposed rats male weaning rats were divided randomly into three groups. The group A was supplemented with 30 mg ?-tocopherol and 15?g sodium selenite per 100gm basal diet. The group B and C were fed basal diet on- ly. The group A and B were kept at the cold room (-2?1℃) and the group C kept at room temperature. Liver mitochondria were isolated for measurement of its respiratory function. Succinate was utilized as substrates of oxidative phosphorylation. Respiration rates of state 3 oxygen uptake and therefore ATP synthesis were found to increase with Vitamin E supplemented diet. Significantly less decline of ADP/O ratios and RCR was observed for group A than that of group B. These results suggest that Vitamin E has the effect of maintaining the physiological intactness of mitochondria of the cold exposed rats, and therefore may enhance the metabolic conservation of energy with the consequence of increasing the ability of cold tolerance of the cold exposed animals.

Blood glucose level responses to different diets were examined in 12 healthy man. The test breakfasts consist of different proportion of protein and carbohydrate. Three kinds of test breakfast were fed respectively for each person. Blood glucose were measured before and 1, 2 and 4 hr after breakfast. The results indicated that high-CHO diet maintained higher level of blood glucose than other diets in 1-2 hr after breakfast. However the blood glucose level began dropping from 2 hr and dropped below fasting level at 4 hr after breakfast. The high-protein diet resulted in decreasing of blood glucose level in 1-2 hr after breakfast. The blood glucose level then increased continuously until 4 hr after breakfast.The results in this experiment showed that adjustment of the proportion of carbohydrate and protein in diets could maintain the blood glucose to a higher and stable level.

Objective To explore the possible effects of nutritional supplements on brain function as reflected by Water Maze test performance in mice after +Gz exposure.Method Mice were arranged into control group (group A),+Gz group without nutritional supplementation (group B) and +Gz plus nutritional supplementation group (group C).Each group contains 12 mice.Mice in group A were not exposed to +Gz while mice in both group B and group C were exposed to 8 min +10 Gz.Distilled water was gavaged to group B mice 3 h before +Gz exposure.On the day before +Gz exposure pyridoxol fortified water was given and 3 h before exposure mixed amino acids solution were gavaged to group C mice.Water Maze test was done and scores were recorded in all groups.After the Water Maze test was completed,blood was collected through the eyes for serum amino acid determinations and brain tissue was collected by decollation for monoamine determination and γ-glutamyl transferase (GGT) activity evaluation. Result After +Gz exposure,longer completion time and more mistakes were observed in Water Maze test in group B as compared with group A and a trend of improvement in group C was noticed. The ratio of brain 5-HT to dopamine(DA) was significantly reduced in group C as compared with group B.Gamma glutamyl transferase (GGT) activity in brain tissue in group C and group B increased significantly. Conclusion High sustained +Gz exposure significantly reduces Water Maze test performance in mice (longer completion time and more mistakes).It seems that there is a trend of improvement in Water Maze performance in mice in dietary nutritional supplementation group,which might be due to significant reduction in ratio of brain 5-HT to DA in mice with nutritional supplementation.

Objective: To compare the clinical effect of metoprolol combined with valsartan and nifedipine controlled release tablets in the treatment of primary hypertension complicated with coronary heart disease(CHD). Methods: 200 primary hypertension patients with CHD were selected, and they were randomly divided into observation group and control group according to the digital table, 100 cases in each group.The observation group was treated with metoprolol combined with valsartan, the control group was treated with nifedipine controlled release tablets.The clinical effect of the two groups was compared. Results: Before treatment, the diastolic and systolic blood pressure of the control group were (106.8±12.3)mmHg and (173.4±22.8)mmHg, respectively, which of the observation group were (104.3±11.4)mmHg, (177.6±24.2)mmHg, respectively, the differences were not statistically significant between the two groups(t=1.265, 0.337, all P>0.05). The diastolic and systolic blood pressure after treatmentin of the observation group were (131.6±17.4)mmHg, (85.3±7.1)mmHg, respectively, and the total effective rate of the observation group was 93.0%(93/100), the improvement rate of angina symptoms of the observation group was 86.0%(86/100), which were significantly better than those of the control group[(131.6±17.4)mmHg, (85.3±7.1) mmHg, 68.0%(68/100), 63.0%(63/100)], the differences were statistically significant (t=5.229, 6.122, χ²=9.148, 7.224, all P<0.05). Conclusion The combination of metoprolol and valsartan has better effect in the treatment of primary hypertension complicated with CHD compared with nifedipine controlled release tablets.

Objective To compare the perio-perative effects of modified bikini minimally invasive approach versus direct lateral approach for total hip arthroplasty(THA).Methods A total of 60 patients(70 hips),including 45 males (49 hips) and 15 females (21 hips) aged (54.9 ± 6.1) years (range from 38 to 63 years)who underwent THA for the first time at joint surgery department of our hospital were retrospectively analyzed from October 2015 to May 2017.Thirty patients(34 hips) underwent modified bikini approach THA(the modified bikini group),and 30 patients(36 hips) received direct lateral approach THA(the direct lateral group).The comparisons were performed between the two groups in the orthopaedic pelvic films,disease stages,Harris scores before and after operation,intra-operative blood loss,postoperative drainage,blood transfusion rate,24-h visual analog scale(VAS) scores for pain after operation,operation time and incision length.The straight leg elevation time,recovery time of hip abduction muscle strength,average hospitalization days and subjective satisfaction of patients were evaluated.Relevant surgical complications were recorded.Results Postoperative X-ray examinations showed that the position of prosthesis was good in both groups.There were no statistically significant difference between the two groups in operation time,intraoperative blood loss,postoperative drainage,blood transfusion rate,straight leg elevation time and subjective satisfaction(P＞0.05).There were statistically significant differences between the modified bikini group and the direct lateral group in the incision length[(9.2±1.9) cm vs.(18.5±3.3) cm],recovery time of hip abductor muscle strength [(1.6 ± 0.8) d vs.(12.1 ± 3.9) d],Harris scores [(87.1±5.9) vs.(86.8 ± 7.1)],24-hour VAS scores [(4.6 ± 2.3) vs.(7.0 ± 3.6)] and average hospitalization days[(7.4 ±1.8) vs.(10.8 ±2.4)]after operation between the modified bikini group and the direct lateral group(P＜0.05).One case with lateral femoral cutaneous nerve injury and 1 case of posterior cortical perforation of proximal femur were found in the modified bikini group,and 1 case of dislocation of hip prosthesis was observed in the direct lateral group.Conclusions Both of the modified bikini minimally invasive approach and direct lateral approach for THA can achieve better postoperative effect,while the modified bikini approach achieves faster recovery.

Objective To explore the effect of amiodarone combined with carvedilol in the treatment of patients with arrhythmia.Methods 90 patients with cardiac arrhythmia were selected .According to different treatment,the patients were divided into control group and observation group ,45 cases in each group.The control group was treated with amiodarone , the observation group was given amiodarone combined with carvedilol .The therapeutic effect of the two groups was observed .Results Before treatment , the systolic blood pressure [ ( 149 ± 11)mmHg vs.(151 ±14) mmHg)],diastolic blood pressure [(95 ±11) mmHg vs.(95 ±12) mmHg)],heart rate [(143 ±16)times/min vs.(141 ±18)times/min] between the two groups had no statistically significant differences (t =6.214, 1.907, 3.228, all P >0.05 ).After treatment, the effective rate ( 91.11％), cardioversion rate (88.89％),standard time to achieve effective treatment [(2.50 ±0.79) d],systolic blood pressure [(119 ± 12)mmHg],diastolic blood pressure[(78 ±12)mmHg],heart rate [(78 ±20)times/min] in the observation group were better than those in the control group [86.67％,75.56％,(5.62 ±3.16) d,(142 ±15) mmHg,(90 ± 13)mmHg,(99 ±23) times/min],and the differences between the two groups were statistically significant (χ2 =8.235,7.157,t =6.214,5.718,5.102,all P<0.05).Conclusion Carvedilol combined with amiodarone in the treatment of patients with abnormal heart rate can significantly improve the cardiac function ,suppress deterioration , and can be widely used in clinical treatment .

With the reform of the medical and health system entering a critical period,public hospitals have also exposed new risks and challenges in economic operation.As an important means of hospital standardized management,internal control can better prevent and resolve the risk of hospital economic operation and ensure the sustainable operation of the hospital.By interpreting the requirements of current national policies on hospital internal control,it analyzes the functional positioning of financial and accounting supervision in hospital internal control,shares the internal control implementation path of sample hospitals from the perspective of financial and accounting supervision,and puts forward suggestions on strengthening internal control construction of public hospitals in the new era,in order to lay a good foundation for the high-quality development of hospitals.

OBJECTIVE To investigate the impacts of isorhynchophylline （IRN） on airway inflammation in asthmatic mice by regulating the monocyte chemotactic protein-1 （MCP-1）/CC chemokine receptor 2 （CCR2） signaling pathway. METHODS The asthmatic mice model was established by injecting and inhaling ovalbumin. The successfully modeled mice were randomly grouped into asthma group， IRN low-dose group （IRN-L， intragastric administration of 10 mg/kg IRN）， IRN high-dose group （IRN-H， intragastric administration of 20 mg/kg IRN）， IRN-H+CCL2 group [intragastric administration of 20 mg/kg IRN+intraperitoneal injection of 7.5 ng CC chemokine ligand 2 （CCL2）] and positive control group （intraperitoneal injection of 2 mg/kg dexamethasone）. The mice injected and inhaled with sterile phosphate-buffered solution were included in the blank control group， with 10 mice in each group. The mice in administration groups were given relevant medicine once a day， for consecutive 2 weeks. The levels of airway hyperreactivity indexes such as enhanced （Penh） value， tumor necrosis factor-α （TNF-α），interleukin-13 （IL-13） and IL-4 in serum， the number of eosinophil （EOS）， lymphocyte （LYM） and neutrophils （NEU） in alveolar lavage fluid and the protein expressions of MCP-1 and CCR2 in lung tissue were observed in each group； the pulmonary histopathological changes were observed， and inflammatory cell infiltration score was evaluated. RESULTS Compared with the blank control group， the infiltration of inflammatory cells in the lung tissue of mice was more significant in the asthma group， and there was swelling and shedding of cells； inflammatory infiltration score， Penh value， the levels of IL-4， IL-13 and TNF-α， the number of EOS， NEU and LYM， the protein expressions of MCP-1 and CCR2 were increased significantly （P＜0.05）. Compared with the asthma group， the pathological injuries of the IRN-L group， IRN-H group and positive control group were improved， and the above quantitative indexes were decreased significantly （P＜0.05）. Compared with the IRN-L group， the above quantitative indexes of the IRN-H group and positive control group were decreased significantly （P＜0.05）. There was no statistical significance in the above quantitative indexes between the IRN-H group and the positive control group （P＞0.05）. Compared with the IRN-H group， the above quantitative indexes of the IRN-H+CCL2 group were increased significantly （P＜0.05）. CCL2 reversed the protective effect of high-dose IRN on asthmatic mice. CONCLUSIONS IRN may reduce the release of airway inflammatory factors in asthmatic mice by inhibiting the activation of the MCP-1/CCR2 signaling pathway， so as to achieve the purpose of improving asthma.