Objective To study the expression of cyclooxygenase-2,epithelial cadherin and vascular endothelial growth factor-c in colorectal cancer and its significance. Methods Seventy-seven patients with colorectal cancer were detected the expression of COX-2, E-ca nd VEGF-c by immunohistochemical SP method. Results COX-2 positive expression in colorectal cancer tissue was 24.7%; E-ca positive expression rate of 68.8%; VEGF-c expression rate of 84.4%. COX-2 in highly differentiated cancer tissues was significantly higher than in poorly differentiated ones (P ＜0. 05); E-ca in the tubular adenocarcinoma was higher than in the mucous glands cancer and other types of cancer ( P ＜ 0. 05 ); The Positive expression rate of E-ca in the stage Ⅰ + Ⅱ was significantly higher than stage Ⅲ + Ⅳ (P ＜0. 005). COX-2 protein expression and VEGF-c expression was negatively correlated (P ＜ 0. 05 ). Conclusion Combined detection of COX-2,VEGF-c and E-ca is helpful to recognize the occurrence, development and tratment of colorectal cancer.

ObjectiveTo investigate the role of ischemic postconditioning on lung ischemic/reperfusion injury induced by cardiopulmonary bypass (CPB) in combined mitral and aortic valve replacement,and evaluate the effects of different protocol of ischemic postconditioning,and analyze its possible mechanisms.Methods24 patients diagnosed as rheumatic mitral and aortic valve disease with or without tricuspid valve disease were randomly divided into 3 groups (8 in each):Control group( group A),patients undergoing routine cardiac surgery; Postconditioning group Ⅰ (group B ),patients undergoing routine cardiac surgery and ischemic postconditioning by occlusion of the pulmonary artery of five cycles of 15 s ischemia and 15 s repeffusion before the pulmonary artery totally restoring peffusion; Postconditioning group Ⅱ (group C),patients undergoing routine cardiac surgery and ischemic postconditioning by occlusion of the pulmonary artery of five cycles of 30 s ischemia and 30 s repeffusion before the pulmonary artery totally restoring perfusion.The Oxygenation Index (OI) was measured at pre -operation and 1 h,2 h,3 h,6 h and 12 h after terminating CPB.The plasma contents of MDA were detected by Enzyme-linked Immunosorbent Assay at pre -operation,1 h,3 h,6 h and 12 h after terminating CPB.ResultsCompared with group A and C,OI in group B significantly increased at 1 h,2 h,3 h,6 h after terminating CPB (283.25 ±56.47 vs 384.76 ±29.17 vs 310.50 ±65.71,265.75 ±58.78 vs 381.75 ±29.67 vs 310.50 ±48.17,283.75 ±73.15 vs389.74±39.34 vs 317.87 ±78.41,310.37 ±52.00 vs 401.62 ±4 2.89 vs 337.25 ± 64.06,all P ＜0.05),and the plasma contents of MDA in group B reduced at 1 h,3 h,6 h,12 h after terminating CPB (4.64±0.63 vs 3.88 ±0.20 vs 4.38 ±0.41,5.75±0.49 vs 4.44 ±0.34 vs 5.28 ±0.76,4.42±0.31vs 3.77 ± 0.40 vs 4.35 ± 0.54,3.74 ± 0.31 vs 3.19 ± 0.17 vs 3.64 ± 0.24,all P ＜ 0.05 ), However,there were no significant differences in OI and MDA between group C and A (all P ＞0.05 ).Conclusions Ischemic postconditioning by occlusion of the pulmonary artery of 5 cycles of 15 s ischemia and 15 s reperfusion attenuates lung oxygenation function injury induced by CPB in double valves replacement,and it is possibly mediated by reducing MDA produced by lipid peroxidation.However,ischemic postconditioning by occlusion of the pulmonary artery of 5 cycles of 30 s ischemia and 30 s repeffusion may not have significant protective effect for lung oxygenation function injury induced by CPB in double valves replacement.

Objective To explore the influence of the protection motivation theory (PMT) on the self-nursing ability of high-risk diabetic foot (DF) patients. Methods The outpatients in our hospital were selected with high-risk DF between January 2013 and May 2014, randomly divided into the control group and PMT group, 52 cases in each group. Guided under the protection motivation theory, PMT group received a six-month health education and management; and the control group accepted conventional health education of diabetes. Before and after the intervention, some observation indexes of the two groups respectively were evaluated including the ability of diabetic foot self-nursing, foot condition, fasting blood sugar, 2 h postprandial blood glucose. Result After six months, the scores of the self-care ability of diabetic foot and foot condition from the patients of PMT group were higher than that of PMT group before the intervention and that of control group after intervention (P<0.05). Conclusion PMT can help patients with high-risk DF enhance their foot self-care ability, improve their foot condition, control their blood sugar, and prevent the DF onset.

OBJECTIVEThis retrospective study is performed to analyze the epidemiological and clinical features of patients with infective endocarditis (IE) hospitalized in Fuwai Cardiovascular Hospital during the latest 7 years.

METHODSThis retrospective study included a cohort of 368 infective endocarditis patients hospitalized in Fuwai Hospital form August 2005 to August 2012. Predisposing cardiac diseases, causative organisms, clinical features and outcomes were analyzed. Risk factors related to outcome including NYHA classes, causative organisms and complications, were evaluated.

RESULTSAmong the IE patients, 6.8% (25/368) patients had rheumatic heart diseases 31.8% (117/368) had congenital heart diseases, 22.8% (84/368) were post-PCI or operative endocarditis and IE developed in 14.1% (52/368) patients without previous cardiac diseases. Blood culture positive rate was 46.2% (170/368). Streptococci viridians [27.6% (47/170) ]were the most common causative organisms, followed by coagulase-negative staphylococci [15.9% (27/170) ]. Fever and cardiac murmur were the most common clinical presentations. Congestive heart failure was the most common complication [87.8% (323/368)]. Systemic and pulmonary embolism occurred in 16.0% patients, 80.9% IE was detected by echocardiography. In-hospital mortality rate was 6.7%, mostly due to refractory congestive heart failure and sepsis. Subgroup analysis showed that incidence of post-PCI or operative endocarditis was significantly higher in IE patients hospitalized after 2009 compared to IE patients hospitalized before 2009 (27.5% vs. 19.2%, P < 0.05) . Higher incidence of staphylococcal infection was evidenced in mechanical valves than in native valves (44.4% vs. 19.8%, P < 0.05).

CONCLUSIONDuring the past decade, there is a significant change on epidemiology and clinical features of IE in China. Incidence of post-surgical and interventional IE increased significantly. Staphylococcal and Gram negative bacilli infection are major pathorganisms of endocarditis of mechanical valves. Due to the lower positive rate of blood culture, echocardiography serves as the most important diagnostic tool for infective endocarditis.

Adult ; Endocarditis ; epidemiology ; microbiology ; Female ; Humans ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Risk Factors