Object To determine the activities of various antioxidant enzymes, including superoxide dismutase, catalase, peroxidase and glutathione peroxidase, and the contents of some simpler antioxidants such as glutathione, chlorogenic acid and certain flavonoids in the leaves, stems and fruits of Viscum coloratum (Kom ) Nakai Methods The activities were determined as described in various literature reports Results The plant was found to be rich in various antioxidants Conclusion V coloratum, with its abundant antioxidant constituents, is proved to be a strong antioxidation drug for medical use especially its leaves

OBJECTIVEThe purpose of this study was to evaluate the effects of squamous cell carcinoma on oral bacteria.

METHODSThis study investigated the microbial contents of the plaque on the surfaces of oral squamous cell carcinomas. Samples were obtained from the central surface of lesions, contiguous healthy mucosa and saliva of 42 patients with oral squamous carcinoma before and after operation.

RESULTSThe median of bacterial colony forming units per milliliter (CFUs/ml) of saliva before operation was 8.10 x 10(8) CFUs/ml. The median of CFUs per square centimeter of the tumor surface was 5.21 x 10(5) CFUs/cm2 which was significantly higher than that of the healthy (the control) mucosa (1.77 x 10(4) CFUs/cm2, P = 0.0001). The CFUs per square centimeter of mucosa surface at the operative zone was 4.34 x 10(5) CFUs/cm2 which was also significantly higher than that of the healthy (control) mucosa(7.24 x 10(4) CFUs/cm2, P = 0.0001).

CONCLUSIONOral carcinoma can break the balance of oral microflora, which may be one of the reasons leading to the high susceptivity of these compromised patients to systemic infection.

Adult ; Aged ; Bacteria, Aerobic ; isolation & purification ; Bacteria, Anaerobic ; isolation & purification ; Bacterial Infections ; etiology ; prevention & control ; Carcinoma, Squamous Cell ; microbiology ; Female ; Humans ; Male ; Middle Aged ; Mouth Mucosa ; microbiology ; Mouth Neoplasms ; microbiology ; Saliva ; microbiology ; Streptococcal Infections ; prevention & control ; Streptococcus ; isolation & purification

Our earlier research has shown that mono-substituted N-phenyl-2, 2-dichloroacetamide exhibited much higher anti-cancer activity than the lead compound sodium dichloroacetate (DCA). In this paper, a variety of multi-substituted N-phenyl-2, 2-dichloroacetamides were synthesized and biologically evaluated. The results showed that 3, 5-disubstituted N-phenyl-2, 2-dichloroacetamide analogues had satisfactory potency. Among them, N-(3, 5-diiodophenyl)-2, 2-dichloroacetamide had an IC50 of 2.84 micromol x L(-1) against non-small cell lung cancer cell line A549 and could induce cancer cell apoptosis.

Objective To explore clinical application effects of hands-off method among puerperas delivered through vagina. Methods To utilize retrospective study design, a total of 1 040 puerperas, who could not received natural labour from July to November 2013, were selected as observation group by convenient sampling method and underwent hands-off method to deliver children, while 1 005 prerperas were taken deliver technical protection of perineum from July to November 2010 as control group. We compared puerperas′delivery conditions and injury of perineum, and observed and analyzed the influencing factors of puerpera′s episiotomy and perineal laceration. Results No incidences of Ⅲ and Ⅳ degree perineal laceration and obstetric anal sphincter injury happened. The rate of puerpera′s episiotomy in the observation group was 25. 38% compared with 13.93% in the control group (P <0.01). The Ⅱ degree of perineal laceration was 48. 85% in the observation group lower than 82. 09% in the control group (P<0. 01). Comparing injury of perineum between two groups, the medium injury of observation group (Ⅱdegree of perineal laceration and episiotomy, 74. 23%) was lower than 96. 02% puerperas of control group (P <0. 01). The rate of vaginal wall hematoma in the observation group was 0. 77% lower than 4. 68% in the control group (P<0. 01). The length of second stage of labor and length of perineum wound suture were shorter than those of the control group (P<0. 05);the amount of bleeding 2 h after labor in two groups had no statistical significance (P>0. 05). Regression analysis showed that, maternal age, analgesia, newborn weight were the influence factors of lateral episiotomy (P <0. 05);maternal age, weeks of gestation, analgesia, newborn weight were the influence factors of perineal laceration (P<0. 05). Conclusions The hands-off method to deliver child can elevate the perineal laceration degree of perineum, reduce the length of the second stage labor, and shorten the length of perineal wound suture.

Objective To evaluate the effect of regional hypothermia on limb ischemia-reperfusion (I/R) injury in a rabbit model of hemorrhagic shock.Methods Eighteen healthy adult New Zealand white rabbits of both sexes,weighing 2.0-2.5 kg,were divided into 3 groups (n=6 each) using a random number table:sham operation group (S group),limb I/R group (I/R group) and regional hypothermia group (RH group).Rabbits were shot with 0.25 g steel ball with a 7.62 mm smoothbore at the middle and lower 1/3 of the left hindlimb in I/R and RH groups.When the blood pressure was stable for 30 min after injury,blood was drawn via the right femoral artery at a rate of 2 ml/min until mean arterial pressure decreased to 45-50 mmHg.Then a rubber tourniquet was used to ligate left hindlimbs 10 min later.The injured limb was cooled down for 4 h after applying tourniquet to maintain the subcutaneous temperature at 9-11 ℃ in group RH.After tourniquet was applied for 4 h,perfusion was restored and lasted 6 h.After catheterization (T0) and at 2,4 and 6 h of reperfusion (T1-3),blood samples were collected from the right femoral artery for blood gas analysis and for determination of tumor necrosis factor-α (TNF-α),interleukin-2 (IL-2) and IL-6 concentrations in serum (by enzyme-linked immunosorbent assay).The animals were sacrificed at T3,and tissues around the margin of the inferior lobe of right lung were obtained for examination of the pathological changes which were scored and for determination of wet to dry weight ratio (W/D ratio).Results Compared with group S,the respiratory rate was significantly increased and PaCO2 was decreased at T2,3,the pathological score and W/D ratio were increased,and the concentrations of TNF-α,IL-2 and IL-6 in serum were increased at T1-3 in I/R and RH groups (P＜0.05).Compared with group I/R,the respiratory rate was significantly decreased and PaCO2 was increased at T2,3,the pathological score and W/D ratio were decreased,and the concentrations of TNF-α,IL-2 and IL-6 in serum were decreased at T1-3 (P＜0.05),and the pathological score was significantly attenuated in group RH.Conclusion Regional hypothermia can reduce limb I/R injury in a rabbit model of hemorrhagic shock,and the mechanism may be related to inhibiting systemic inflammatory responses.