OBJECTIVE:To investigate the drug treatment of hospitalized patients with depression in our hospital during 2008. METHODS: In cross section study, the medical records which in accordance with ICD-10 diagnostic criteria for depression disorders in our hospital during 2008 were retrieved, then the utilization of psychotropic drugs was analyzed and summarized. RESULTS: The utilization rate of new antidepressants including selective serotonin reuptake inhibiter (SSRI), selective serotonin and noradrenaline reuptake inhibiter (SNRI), noradrenaline and specific serotonin antagon (NaSSA) reached 89.9%, among which sertraline accounted for the largest proportion (22.5%). New antidepressants combined with atypical antipsychotics took up a big proportion, particularly quetiapine (39.2%). CONCLUSION: New antidepressants become the mainstream drug in the treatment of depression. Drug combination occupies a big proportion, especially combining with atypical antipsychotics. The safety of drug combination need to be further explored.

Objective To realize objectification and detailed quantification of palpation.Metbods A kind of automatic palpation device was developed to meet the needs of safeand easy operation,technique simulation,and multi-parameter monitoring in real time.Results This device mainly consisted of programmable controller and its analog-to-digital converter module,mechanical positioning device,pressing device,force testing device and hand-held touch screen,and it was designed to be operated by attenders independently.The device could collect multiple data including force,time and distance in the process of palpation,and monitor the force thresholds of pressing responses at all levels to achieve desired effects.Conclusion This device can be used in the automatic palpation for suitable body areas,especially in the comprehensive observation of acupoint-pressing responses or soft tissue tenderness.

OBJECTIVE:To compare therapeutic efficacy and safety of budesonide or beclometasone combined with terbutanline in the treatment of acute attack of mild and moderate asthma in children.METHODS:A total of 110 children with mild and moderate asthma were randomly divided into group A (55 cases) and group B (55 cases).Based on routine treatment,group A was given Terbutaline sulphate solution for nebulization 1 mL,nebulization inhalation,twice a day+Budesonide suspension for inhalation 2 mL,nebulization inhalation,twice a day.Group B was given Terbutaline sulphate solution for nebulization (same usage and dosage as group A)+Beclomethasone dipropionate suspension for inhalation 2 mL,nebulization inhalation,twice a day.Treatment courses of 2 groups lasted for 7 d.Clinical efficacies,symptoms and signs disappearance time,hospitalization time,asthma relief rate as well as symptoms and signs scores,peak value expiration velocity of flow (PEF),the occurrence of ADR were observed in 2 groups.RESULTS:There was no statistical significance in total response rate,symptoms and signs disappearance time,hospitalization time,asthma relief rate or the incidence of ADR between 2 groups (P＞0.05).After treatment,symptoms and signs scores of 2 groups were significantly lower than before,while the PEF were significantly higher than before,with statistical significance (P＜0.05),but there was no statistical significance between 2 groups (P＞0.05).CONCLUSIONS:Based on routine treatment,budesonide or beclometasone combined with terbutaline sulphate can be used for acute attack of mild and moderate asthma in children.They are similar to each other in therapeutic efficacy and safety.

Objective To study the change of serum diamine oxidase (DAO) level in patients with severely acute pancreatitis (SAP) in order to explore the role of DAO in assessing the severity of SAP and the magnitude of gastrointestinal dysfunction.Methods From January 2012 through December 2013,56 SAP patients with 33 male and 23 female and average age (45-± 14) years admitted within 3 days after onset were enrolled for this study.At admission,serum diamine oxidase (DAO) was detected,and APACHE Ⅱ score,computed tomography severity index (CTSI) score and Balthazar grading and gastrointestinal dysfunction score were calculated.And at the corresponding time,serum procalcitonin (PCT) was detected.The correlations between serum DAO level and 4 other markers were analyzed.Results The high level of serum DAO was found at admission in SAP patients correlating positively with serum PCT concentrations (r =0.516,P＜ 0.01),APACHE Ⅱ score (r =0.631,P＜ 0.01),CTIS score (r=0.640,P ＜ 0.01),and the degree of gastrointestinal dysfunction (r =0.730,P ＜ 0.01).Conclusions The role of serum DAO in assessing the severity of SAP and magnitude of gastrointestinal dysfunction in SAP patients is really valid.

Objective To observe the effect of Astragalus Injection(AI) on aquaporin-1(AQP-1) expression of peritoneal mesothelial cells in rats with peritoneal dialysis.Methods Twenty-five male SD rats were divided into the normal control group(N =7),model group(N =9) and AI group(N =9).Except the normal control group,the model group was given intraperitoneal injection of peritoneal dialysate(containing glucose 42.5 g/L),and AI group was given intraperitoneal injection of peritoneal dialysate(containing glucose 42.5 g/L) plus AI(20 mg/mL),25mL/d for 10 continuous days.On the 11th day of experiment,the amount of dialysis ultrafiltration(UF) and net UF,and the concentration ratio of dialysate and plasma sodium(D/PNa) were measured,peritoneal pathological changes were observed with light microscope,and the expression of AQP-1 in peritoneal mesothelial cells was detected with immunohistochemical method.Results When the peritoneal dialysate was kept in the abdominal cavity for 60 min,the amount of UF and Net UF was increased in AI group(P

Adult ; Aged ; Aged, 80 and over ; Equipment Design ; Female ; Femur Neck ; surgery ; Fracture Fixation, Internal ; instrumentation ; Humans ; Male ; Middle Aged

Objectives To evaluate modified transthoracic esophageal transection (modified Walker's procedure) for management of postoperative rebleeding of portal hypertension.Methods Since 1983,we adopted modified Walker's operation to make low esophageal transection and transect intraabdominal recurrent varix of the coronary vein in the treatment of esophageal variceal rebleeding in portal hypertensive patients who had received previous surgeries aiming at portal hypertension.Results In 53 cases of postoperative rebleeding of portal hypertension,49 cases underwent elective operation with no mortality,4 cases received emergency surgery,one died post-operatively due to hepatic failure.The postoperative control rate of recurrent bleeding was 100％,and the overall postoperative mortality was 2％.The longest postoperative survival time was 24 years,and the 5-year survival rate was 77％.There were no postoperative recurrent hemorrhage and hepatic encephalopathy.Conclusions Modified transthoracic esophageal transection (modified Walker's procedure) is easy to perform,good at saving operative time,with a reliable immediate hemostatic effect and long-term control of variceal hemorrhage,hence is a effective remedy operative method for postoperative rebleeding of portal hypertensive patients after failed previous operations.

Objective:To explore the changes of cerebral blood flow (CBF) perfusion caused by N 2O (laughing gas) abuse. Methods:From December 2017 to October 2018, the CBF perfusion images of 24 patients with laughing gas abuse (9 males, 15 females; age: 18-32(24.0±8.9) years) from China-Japan Friendship Hospital were analyzed retrospectively. The region uptake statistics of different brain regions of interest (ROI) (basal ganglia, central region, cerebellum, cingulate gyrus, frontal lobe, medial temporal lobe, occipital lobe, parietal lobe, temporal lobe) between patients with laughing gas abuse and normal people of the same age group from background software database were calculated automatically. Statistic>1.68 indicated the increase of local CBF perfusion, while statistic < -1.68 indicated the decrease of local CBF perfusion. The correlation between the statistics of bilateral brain regions and the correlation between statistical values and clinical indicators were analyzed by Pearson and Spearman correlation analyses.Results:The correlation of the statistics between bilateral regions of each brain area was significant( r values: 0.503-0.892, all P<0.05). The decreased CBF proportions of frontal and temporal lobes were 62.5%(15/24) and 70.8%(17/24), respectively. The highest proportion of increased CBF was cingulate gyrus (33.3%, 8/24). There were significant correlations between frontal lobe, central brain area and duration of laughing gas abuse ( rs values: 0.375, 0.305, both P<0.05). Conclusion:CBF perfusion imaging is helpful for understanding the changes of CBF in patients with laughing gas abuse.

Objective To investigate the T cell cytotoxicity induced by recombinant adenovirus carrying HIV-1 vpr gene.Methods C8166 cells infected with rAd-vpr or negative control rAd-vector,were analyzed for cell cycle distribution and cell death by flow cytometry.The discrimination of living cells,apoptotic and necrotic cells were differentiated with Hoechst-PI double staining under the confocal microscopy.Changes of mitochondrial membrane potential(△ψm)were monitored by JC-1 staining method.Results Annexin V-PI and Hoechst-PI staining indicated the death effects of HIV-1 Vpr on C8166 cells.PI flow cytometric analysis showed that cell cycle arrested in G2 phase.C8166 cell△ψm collapse mediated by Vpr was detected by JC-1 fluorescent staining.Conclusion The ability of recombinant adenovirus carrying HIV-1 vpr gene to induce mitochondria dysfunction,cell cycle G2 phase arrest and cell death was confirmed in C8166 cells.

AIM: To study the effects of [8-(diethylamino) octyl-3, 4, 5 -trimethoxybenzoate] (TMB-8), an intracellular Ca2+ antagonist, on the activation, proliferation and cell-cycle distribution of the mouse T lymphocytes stimulated by concanvalin A (Con A) in vitro. METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A (CsA). The expression of CD69, the early marker of CD3+ T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester (CFDA-SE) flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.RESULTS: After 6 h culture, the activation rate of CD69+ T cell in Con A group was (74.88?1.88)%. 10, 20 and 40 ?mol/L of TMB-8 inhibited the expression of CD69 (P