Objective To investigate the separation rate ,clinical distribution ,antibiotic susceptibility rate of Staphylococcus au‐reus from skin and soft tissue infections and provide the reference for clinical diagnosis and treatment .Methods Retrospective sta‐tistical analysis of 214 strains of Staphylococcus aureus from skin and soft tissue infections from September 2011 to August 2014 by Whonet 5 .6 .Results 1 228 pathogens were isolated from 2 152 cases of the secretion of operative and skin wound ,of which there were 214 strains of Staphylococcus aureus ,which accounted for 17 .43% ,48 strains of MRSA ,which accounted for 22 .43% ;Mainly distributed in bone surgery ,burns orthopaedic ,breast surgery ,with skin venereal division ,accounting for 40 .2% ,22 .3% ,12 .1% , 8 .4% respectively .The resistance rate of Staphylococcus aureus to penicillin ,erythromycin ,clindamycin were 96 .3% ,46 .7% , 37 .4% respectively ,high sensitivity to vancomysin ,linezolid ,fusidic acid ,mupirocin .Conclusion Surgical infection rates of SSTIs are high in our hospital .It should be according to SAU susceptibility analysis results to choose high sensitivity of the first and sec‐ond generation cephalosporins for treatment in clinic ,and vancomycin ,linezolid only in severe Staphylococcus aureus(including MR‐SA) infection to choose .

Objective To study the values of serum CA19-9,CA242,CEA,alone or in combination in the diagnosis and prognosis of combined hepatobiliary calculus and cholangiocarcinoma (HCWC).Method Serum CA19-9,CA242,CEA in 100 patients with HCWC,70 patients with hepatobiliary calculus combined with cholangitis and 30 patients with hepatic hemangioma (normal bile duct group) were preoperatively studied.Results When the serum levels of CA19-9,CA242,CEA were separately used in the diagnosis of HCWC,the sensitivity of CA19 9 was highest,but its specificity was significantly lower than that of CA242 and CEA (P＜0.01).Patients with all the three tumor markers raised had significantly lower survival than those of patients with only one or two raised markers (P＜0.05).Conclusions The diagnostic rate for CA19 9 in HCWC was better than that of CEA and CA242.A joint detection improved the diagnostic specificity.Raised tumor markers were associated with progression of HCWC.Survival was worse in patients with 3 raised markers than those with 2 or 1 raised markers.

Objective To investigate the clinical application value of point-of-care combined detection of cTnI,MYO and CK-MB in the patients with acute myocardial infarction (AMI).Methods 74 cases of coronary syndrome were collected as the patients group,including 36 cases of AMI and 38 cases of unstable angina pectoris(UAP),and 40 individuals with healthy physical examina-tion were selected as the control group.The concentrations of cardiac marker cTnI,MYO and CK-MB in blood at different times were detected by using the point-of-care rapid immune quantitative method.Then the sensitivity and specificity for diagnosing AMI were compared for determining the best time of single detection and combined detection.Results The positive rates of cTnI,MYO and CK-MB within onset 2-12 h with the symptoms in the AMI group were higher than those in the control group and the UAP group,the difference had statistical significance (P <0.01).The combined detection of cTnI,MYO and CK-MB within onset 6-12 h with symptoms had the higher sensitivity and higher specificity for diagnosing AMI.The combined detection of cTnI and CK-MB within onset 12-24 h with symptoms could reach the optimal sensitivity and optimal specificity for the diagnosis.The sensitivity and specificity of cTnI within onset 24-72 h with symptoms for diagnosing AMI were 100.0% and 100.0% respectively.Conclu-sion The point-of-care combined detection of cTnI,MYO and CK-MB can conveniently and rapidly diagnose AMI.The sensitivty and specificity of various indexes in different time periods are different.Their combined detection can increase the diagnostic rate for AMI.

Objective To investigate ACEI ( benazepril ) and tranilast exert renoprotective properties in diabetic nephropathy( DN) through the inhibition of thioredoxin( Trx) . Methods Forty male SD rats were randomly divided into normal control group,model control group,tranilast group and benazepril group (n=10 each).Normal control group was fed with normal diet. Other groups were fed with high-glucose high-fat diet to make DN models. Rats in model control, tranilast, and benazepril groups were fed with normal diet,400 mg??kg-1??d-1 tranilast plus normal diet,and 10 mg??kg-1??d-1 benazepril plus normal diet,respectively,via oral gavage for 12 weeks.The 24-hour proteinuria,blood glucose(BG),blood urea nitrogen (BUN),serum creatinine ( Scr) and renal pathology changes were detected. Expression of Trx was measured by Western-blot. Results The 24 h urine protein, BG, BUN, Scr, kidney/body weight, and glomerular sclerosis index were significantly decreased in tranilast group and benazepril group,as compaired with model control group ( P<0.05) ,but there was no statistical difference between the two drug groups (P>0.05).Both tranilast and benazepril can reduce renal pathological changes,and can increase the expression of Trx of DN rats, but benazepril had a more significant effect on increasing Trx expression. Conclusion Both tranilast and benazepril have renoprotective function in DN, and benazepril is more effective than tranilast in delaying the progression of diabetic nephropathy by increasing Trx expression and decreasomg oxidative stress.

Recently,optical biometers based on swept-source optical coherence tomography (SS-OCT) technology have been applied to clinical ophthalmology.Compared with previous optical biometers,SS-OCT instruments have many advantages,such as faster processes,more accurate results,higher detection rate,and more built-in formulas for calculating the intraocular lens (IOL) power;moreover,SS-OCT images can be used to detect preliminary maculopathy and predict postoperative tilt of IOL.In this paper,we reviewed the application of SS-OCT biometer in cataract,mainly focus on the principle of work,the evaluation of repeatability and reproducibility,the consistency comparison with other biometers,and the application of SS-OCT in other aspects of cataract.

Objective: To evaluate the repeatability and reproducibility of swept-source optical coherence tomography biometrics (Tomey OA-2000) for measuring the biological parameters of the eyeballs in healthy people. Methods: A diagnostic test was carried out in this study.One hundred and three healthy volunteers who were recuited from July to September 2018 in Affiliated Hospital of North Sichuan Medical College were included, and the right eyes were examined.Two examiners used OA-2000 for 3 consecutive measurements, respectively.One week later, the first examiner used OA-2000 for 3 consecutive measurements.The ocular biological parameters including axial length (AL), anterior chamber depth (ACD), corneal curvature (K), lens thickness (LT), central corneal thickness (CCT), pupil diameter (PD), and white to white (WTW) were measured.The repeatability and reproducibility of each parameter based on within-subject standard deviation (S_W), repeatability limit of standard deviation (2.77S_W), coefficient of variation (CoV) and intra-class correlation coefficient (ICC) was evaluated.This study followed the Declaration of Helsinki.This study protocol was approved by Ethic Committee of the Affiliated Hospital of North Sichuan Medical College (No.2018ER[A]019). Results: Of the ocular biological parameters measured by OA-2000, CoV was less than 2% while ICC was greater than 0.950, except PD and WTW.The repeatability and reproducibility of K, AL and ACD were all good.For K₁(ø=2.5 mm and 3.0 mm), the S_W was less than 0.120 D, the 2.77S_W was less than 0.350 D, the CoV was less than 0.293%, and the ICC was greater than 0.990; For K₂ (ø = 2.5 mm and 3.0 mm) , the S_W were less than 0.175 D, the 2.77S_W were less than 0.485 D, the CoV was less than 0.396%, the ICC was greater than 0.980; for K_m (ø = 2.5 mm and 3.0 mm), the S_W were less than 0.099 D, 2.77S_W were less than 0.275 D, CoV was less than 0.227%, ICC was greater than 0.996; ACD S_W was less than 0.024 mm, 2.77S_W was less than 0.066 mm, CoV was less than 0.668%, ICC were greater than 0.993; the S_W of AL was less than 0.014 mm, the 2.77S_W was less than 0.038 mm, the CoV was less than 0.057%, and all of the ICCs were 1.000. Conclusions Except PD and WTW, the ocular biological parameters measured by OA-2000 have good repeatability and reproducibility, in which the accuracy of AL measurement is the highest.

Objective To investigate the clinical features and viral load of persons infected with HCV and the risk factors for severe outcomes.Methods Medical testing and questionnaire survey were conducted on 465 cases who were infected with HCV,20-30 years back.HCV RNA,alanine transaminase (ALT),aspartate transaminase (AST),albumin,globulin and bilirubin were tested for these subjects.Factors as demography,tobacco and alcohol consumption,SNP of rs7453920 and rs2856718 on HLA-DQ gene of subjects with HCV RNA,were analyzed by multiple logistic regression method to explore the risk factors for severe outcomes among the patients.Result Totally,465 subjects had symptoms as hypodynamic (15.70％,73/465),digestive system (17.63％,82/465),and arthrodynia (10.32％,48/465).HCV RNA was positive in 68.60％ (319/465) of the subjects with median viral load as 76.01 × 104 copies/ml (min-max:592 copies/m1-1.08 × 1010 copies/ml).Totally,11.83％ (55/465) of the cases appeared having liver inflammation by routine ultrasound exams.ALT and AST was seen higher than 80 (IU/L) in 12.70％ (59/465) and 11.18％ (52/465) of the subjects,separately.Factors as being male (OR=2.298,95％CI:1.247-4.238),GA genotype compared with AA type in rs2856718 (OR=1.716,95％CI:1.070-2.752),alcohol intake ≥7 times per-week (OR=2.966,95％ CI:0.979-8.988) etc.,were independently related to HCV RNA sustained positivity.Factors as:being male (OR=1.694,95％CI:0.975-2.942),in 50-59 years age group (OR=2.414,95％ CI:1.156-5.042),having other liver diseases (OR=2.592,95％CI:1.105-6.079) and carrying positive HCV RNA (OR=3.479,95％CI:1.648-7.343) etc.were independent risk factors for abnormal liver function.Conclusion High rates of carrying sustained positive HCV RNA and abnormal liver function appeared in subjects who got the HCV infection 20-30 years ago.Factors as being male,in old age,being frequent alcohol taker,GA genotype in rs2856718 and with other liver diseases etc.were related to higher risk for developing severe outcomes.

Objective To explore the safety and feasibility of microwave ablation assisted laparoscopic resection of liver cancer.Method The clinical data of 40 patients with liver cancer were retrospectively analyzed from April 2013 to June 2016 in Shengjing Hospital.Results Procedures were completed successfully without conversion to open laparotomy or serious complications.The average operation time,blood loss and postoperative hospital stay were (160 ± 68) min,(36 ± 27) ml and (7.6 _± 2.7) d,respectively.There was no tumor recurrence in surgical margins.The postoperative median tumor-free survival was 30 months,with cumulative 1-year and 2-year tumor-free survival rates of 89.4％ and 65.5％,respectively.The postoperative median overall survival time was 38 months,with cumulative 1-year and 2-year survival rates of 100％ and 90.9％,respectively.Conclusion Microwave ablation can effectively control intraoperative bleeding,and prevent tumor recurrence in surgical margins.Microwave ablation assisted laparoscopic hepatectomy is safe and feasible for hepatocellular carcinoma.

Objective To analyze the molecular characteristics and genetic origin of a novel avian influenza A H7N4 virus casuing a case of human infection in China. Methods Specimens were collected from the patient and chickens and ducks kept by the patient and neighbours and then detected by real-time quantitative PCR. The original specimens and virus isolates were analyzed by next-generation sequencing technology to obtain viral whole-genome sequences. Pairwise sequence alignments and phylogenetic analysis were performed by BLASTs,ClustalX and MEGA 6. 1 softwares. Results In January 2018, a human case infected with avian influenza A H7N4 virus was confirmed. Seven H7N4 viruses were isolated from speci-mens collected from chicken and ducks kept in the patient`s backyard. H7N4 virus was a novel reassortant vi-rus with all eight gene fragments derived from wild waterfowl in Eurasia. HA protein contained a single basic amino acid residue R in cleavage site, suggesting that H7N4 virus was low pathogenic. The receptor-binding sites of HA had QSG at 226-228 residues, which indicated that the virus retained avian-type receptor speci-ficity (SAα2-3Gal). Different from H7N4 viruses in avian, the virus isolated from the patient had substitu-tion at position 627 ( E→K) in PB2 protein, which might increase its adaptation in human host. Conclusion This study reported a case of human infection with a novel reassortant avian influenza A H7N4 virus, which revealed that the traditional backyard breeding models might facilitate cross-species transmission of avian in-fluenza viruses in southern China.

COVID-19 is a public health emergency currently. In this study, a scale-free network model is established based on the Spring Migration data in 2020.The cities is clustered into three different modules. The epidemic of the cities in the black module was the most serious, followed by the red and the cyan. The black module contains 9 cities in Zhejiang province and 8 cities in Guangdong province, most of them located in the southeast coastal economic belt. These cities should be the key cities for epidemic prevention and control.