Objective To study the effect of continuous passive motion (CPM) on the healing of man made full thickness defects of articular cartilage in the knee of rabbit. Methods A diameter of 3.2mm full thickness defect of articular cartilage in the facies patellaris of femur were made in 60 knees of rabbits. Then the rabbits were randomly divided into groups A,B and C. The animals in groups A and B were treated with CPM for 8 hours and 2 hours daily, respectively, for 4 weeks, while those in group C kept in cage for free activity only. Half of the animals of every group were sacrificed at the end of the 4th and 12th weeks. The H.E. staining, safranin O staining and immunohistochemical analysis of collagens I and II were conducted, the gross appearance and histological change were observed and scored. Results There was significant difference with regard to the score of gross appearance and histological change among groups A, B and C. Conclusion The healing of full thickness defects of articular cartilage in rabbits treated by CPM is better than that in rabbits without CPM treatment. CPM can promote tissue repair and stimulate synthesis of collagenⅡand the glycosaminoglycan.

Objective To study the effect of con tinuous passive motion (CPM) on joint function after arthroscopy in the knee jo int. Methods Arthroscopy examinations and operations were performed in 53 patients. They were divided into study and control groups based on the use or not of CPM immediately in the postoperative period. The cont rol group consisted of 25 patients treated without postoperative CPM and the stu dy group consisted of 28 patients treated with CPM postoperatively. Range of mot ion (ROM) and the comprehensive function score of the knee joint were evaluated preoperatively, at discharge and at three months after operation. Results There were statistically significant differences in the f unction score of the knee joint between the two groups at discharge. The mean f lexion and extension in the study group were 90.64??16.57? and -0.71??3.78 ?, respectively, compared to 69.40??15.17? and -4.32??4.71? in the contro l group. The mean of the comprehensive function score of the knee joint in the c ontrol group were 11.24.compared to 8.89 in the study group. Seventy-five perce nt (21 cases) of the patients in the CPM group and 24% (6 cases)of patients in t he control group had achieved 90? of flexion at the time of hospital discharge. Conclusion CPM had enhanced the therapeutic effects of arthroscopy in the knee joint and shortened the length of stay of the patie nts in the hospital. Further investigation is needed for evaluating the long-te rm effect of CPM.

Diabetic peripheral neuropathy (DPN) is the most common complaint of diabetic patients, affecting the quality of life of patients seriously. The effect of DPN treated with traditional medicine therapy is poor. The rehabilitation of DPN develops fast recently. The authors overview the current trend of the rehabilitation treatments of DPN in the article including medicine therapy, physical therapy, exercise therapy, traditional Chinese medicine, hyperbaric oxygen therapy, orthopaedic devices and so on.

This paper is on effect of mobilization on immobilized ligament.Eighteen rabbits were divided into three groups.Group Ⅰ,eight weeks of active movement;group Ⅱ,eight weeks of passive+active movement;group Ⅲ,age matched normal control.The biomechanical testing,histological and ultrastructural evaluation were used.Results:with mobilization,the structural properties of ligament bone complexs of MCL obviously improved but did not return to normal.The material properties of MCL was similar to those of contralateral and normal control.Arrangement of collagen fibers was similar to that of normal.Resorption of bone in ligament bone insertion sites was not significantly changed.Through mobilization following immobilization,failure location of MCL changed.Conclusion:The recovery of material properties of ligament substance was more quick than that of structural properties of ligament bone complexs.The effect of active movement and passive +active movement on recovery of structure of immobilized ligament was similar.

Objective To observe any effects of pulsed electromagnetic fields (PEMFs) on the expression of transforming growth factor beta-1 (TGF-β1) after sciatic nerve injury and to investigate the possible mechanism of any regeneration of the injured sciatic nerve.MethodsForty-eight SD rats were randomly divided into a PEMF treatment group,a model group and a normal control group with 16 rats in each group.The three groups were then sub-divided into 1 day,3 day,7 day and 14 day subgroups.The rats of the model and treatment groups were clamped to produce a sciatic nerve injury model.The treatment sub groups were exposed to a 9 mT PEMF at 14 Hz for 2 hours once daily for 1,3,7 and 14 days,respectively.The model group was given sham exposure and the normal control group was reared conventionally and not given any special treatment.The histological changes in the rats' sciatic nerves were observed under a light microscope after hematoxylin-eosin staining.Expression of TGF-β1 was detected by immunohistochemical methods.ResultsAfter 7 and 14 days of treatment,Wallerian degeneration of the sciatic nerve in the treatment group was more obvious than in the model group.The expression of TGF-β1 increased during the treatment process and reached a maximum at the 14th day after nerve injury.The expression of TGF-β1 had increased significantly in the model and treatment groups compared with the control group at all observation time points.At the 3rd,7th and 14th day after the operation,the expression of TGF-β1 in the treatment group was significantly higher than that in the model group. Conclusion PEMFs can accelerate Wallerian degeneration of peripheral nerves and can up-regulate the expression of TGF-β1 after sciatic nerve injury,at least in rats.

Objective To investigate the effect and mechanisms of the Berberine on the human breast adenocarci-noma cell line MCF-7 . Methods MTT method was used to evaluate the proliferation effect of MCF-7 and the per-centage of apoptotic cells were determined by flow cytometric analysis. The expressions of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, Cleavage-PARP and Cleavage-Caspase3 were detected by Western blotting. Results The results showed that BBR treatment decreased the activation of JAK2/STAT3 signal pathway and up-regulated the expression of Bax, Caspase3 and PARP activation with the decrease of the expression of Bcl-2. Wherefore, ex-pression of the constitutively active form of STAT3 could attenuate the effect of BBR on the MCF-7 cell. Conclusion Berberine can induces apoptosis and proliferation inhibition of breast adenocarcinoma cell lines of MCF-7 through inhibition of the JAK2/STAT3 signaling pathway.

Objective To investigate the development of rehabilitation medicine, the knowledge, ability and quality requirements of stu-dents in different professional positions, and the demand for rehabilitation industry personnel in Hunan, China, and to discuss the strategies for improving the quality of vocational education. Methods 87 directors of personnel management or rehabilitation department in all levels of hospitals and 100 rehabilitation therapists were included from May to Semptember 2014. The rehabilitation medical service, education background of employees, professional positions, and the demand for rehabilitation knowledge, ability, and industry personnel were sur-veyed. Results and Conclusion Rehabilitation medical service was not sufficiently carried out for insufficient number of employees, unbal-ance structure and other deficiencies. Rehabilitation personnel training schools and enterprises need to strengthen the depth of integration, and improve the professional quality and clinical ability.

AIM To investigate the effects of apoptosis and the expression of telomerase hTERT mRNA activity of HL-60 cells treated with As 2O 3 in different concentrations. METHODS HL-60 cells was cultured with As 2O 3 in different concentrations for 48 hours, then, cell apoptosis was detected with flow cytometry and the expression of telomerase hTERT mRNA was determined with RT-PCR respectively. RESULTS The percentage of the apoptosis in HL-60 cells were 2 28%?0 40%?2 55%?0 22% and 47 57%?2 79% respectively, as the cells were treated with 0 1 ?mol?L -1,1 0 ?mol?L -1,5 0 ?mol?L -1 As 2O 3 for 48 hours. The difference was significant between 5 0 ?mol?L -1 and 0 1～1 0 ?mol?L -1 groups(P

With increasing attention to pains, the measurement of pain threshold has become an important part among researches to pains. Therefore, measuring Methods and their validity as well as reliability have direct influenced the Conclusion of the researchs. The approaches of pain threshold measurements, and the factors influenced the result were discussed in this paper.

目的探讨互动式教育对康复治疗系实习生在运动疗法实习期间的学习效果。方法两组学生分别采用传统教学法和互动式教学法带教实习,实习结束,比较两组学生出科测试成绩。结果接受互动式教学法的学生成绩明显优于传统教学法(P<0.01)。结论互动式教学法可提高康复治疗系实习生的教学效果。