With the development of immunosuppressant and surgical technique,both the survival rate of liver transplant patients and grafts improve significantly. Meanwhile,infection has become one of the most common complication and main reason of death.The knowledge of the characteristics of infection after liver transplantation is helpful for early diagnosis and treatment,which may decrease the mortality.As the clinical manifestation becomes atypical for the usage of immunosuppressant, it's reasonable to combine some specific biomarkers for early diagnosis.Pathogen varies according to the time post transplant,and it can be divided into three stages.Low immune status and the application of broad-spectrum antibiotic are the reason of opportunistic infection and super infection. Epidemiological survey found that new type of fungus and multi-drug resistance bacterium increase.It's important to pay attention to the management and standardized usage of antibiotic,as well as to establish relative guideline,which may decrease the drug resistance and improve outcome.

Objective To compare the antiviral efficacy and economics between lamivudine and entecavir in patients with low preoperative HBV replication after liver transplantation. Methods Liver transplantation patients from 2006 July to 2013 July were selected for being studied prospectively. Statistically evaluated between entecavir and lamivudine efficacy by the analysis of postoperative follow-up of seroconversion and HBV reinfection. Results The cumulative overcast rate of entecavir group was 98.04% one month after transplatation. And 3 of 51 cases were reinfected with average turn time of 9.73 days. Meanwhile , the cumulative overcast rate of Lamivudine group was 100% and 3 of 26 cases were reinfected with average turn time of 5.11 days. There are no statistical differences between the two groups accumulation rate , reinfection rates , as well as cumulative infection rates. Conclusion In the cases of low hepatitis B virus replication before liver transplantation, Entecavir and Lamivudine have the similar effect of preventing HBV reinfection after operation. Compared with the cost of Entecavir group, Lamivudine is more economy.

Objective To assess the effects of liposomal prostaglandin E1 (Lipo PGE1) on hepatic arterial and portal vein inflow early after orthotopic liver transplantation (OLT).Methods Fifty-six patients were divided randomly into two groups who received a primary liver transplant: normal control group and PGE1-treated group. Lipo PGE1 was infused by 20??g/d from day 1 to 14 after OLT in the PGE1-treated group. Measurements of hepatic arterial and portal vein inflow were taken on post-operative day 1, 3, 5, 7, 9 and 14 by color Doppler image (CDI).Results In control and PGE1-treated groups, hepatic artery velocity (HAV) was gradually increased during postoperative 2 weeks, but portal vein flow volume (PVFV) showed a decreased trend. HAV in PGE1-treated group was higher than normal control group during postoperative 5 days, but there was no significant difference in PVFV between two groups.Conclusion Lipo PGE1 can improve hepatic arterial inflow early after OLT, but not significantly influence portal vein inflow.

Objective To study the clinical feature and treatment of cytomegalovirus(CMV) pneumonia in the orthotopic liver transplantation(OLT) recipients.Methods The clinical data of 451 patients after OLT in our hospital from Oct.2003 to June 2005 were retrospectively analyzed.The prevention,clinical feature and treatment of CMV pneumonia were discussed.Results Seven patients((1.66 %)) developed CMV pneumonia after OLT.The symptoms presented much earlier than signs.All patients had high fever,and followed with cough,polypnea,dyspnea one week later.Blood-gas analysis showed hypoxemia.CMV pp65 antigen detection was helpful for early diagnosis.All the infected patients received ganciclovir and foscarnet treatment.At the same time,immunosuppressive drugs were withdrawn and complex treatment including high dose immunoglobulin,low does thymulin and broad-spectrum antibiotec were given.Six patients((85.7 %)) were cured.Conclusions The early onset of CMV pneumonia is not specific but still regular.CMV pp65 antigen detection is specific and sensitive.Ganciclovir and foscarnet treatment is effective and complex therapy necessary for CMV pneumonia.

ObjectiveTo establish early diagnosis of vascular complications following orthotopic liver transplantation (OLT).MethodsThe surveillance and diagnosis making of vascular complications in 53 cases of OLT patients from April 1990 to June 2000 were carried out by color Doppler imaging (CDI), selective-angiography and analyzing relevant clinical features.ResultsThe incidence of vascular complication after OLT is 15%(8/53) including 3 cases of hepatic artery thrombosis, 2 of hepatic artery stenosis, 1 of celiac artery stenosis, and 2 of inferior vena cava stenosis. Diagnosis was confirmed by selective angiography in 7 cases and by autopsy in one. The sensitivity and specificity of CDI were 100%(8/8) and 98% (45/46) respectively. Conclusions Clinical feature of vascular complications following OLT lacks specificity. Continuous monitoring using CDI is a sensitive and specific method in the detection and diagnosis of vascular complications.Duration of surveillance should be no less than 2 months.

0.05 ). Conclusion Individual immunosuppression regime can prolong the survival of the recipients with high risk factors and is proved to be more safe and reliable.

Objective To explore the value of color Doppler image (CDI) in monitoring of portal vein (PV) complications following orthotopic liver transplantation (OLT).Methods Pre and post operative CDI examinations were performed on 107 orthotopic liver transplants. Monitoring parameters of CDI included: portal vein diameter (PVD), portal vein velocity (PVV), portal vein flow volume (PVFv), wave form and spectrum of PV, the presence of collaterals and ascites. Results A total of 4 ( 3.7% ) PV complications were identified by PV angiography in 107 recipients, including 2 cases of portal vein stenosis (PVS), 1 PVS combined portal vein thrombosis (PVT), 1 right portal vein (RPV) occlusion. Diagnostic sensitivity and specificity for PV complications of CDI were 100 % (4/4) and 98.1% (101/103), respectively. We performed percautaneous transluminal angioplasty (PTA) in 2 patients with PVFv remarkably reduced, and conservative treatment in 2 patients without PVFv reduced.Conclusion CDI is a sensitive and specific way of monitoring portal vein complications following OLT and also contributes to the treatment of portal vein complications. The conservative treatment might be effective for the patients with PVFv reduced unremarkably and the presence of collaterals, if not, PTA should be performed as early as possible.

Objective To analyze the high risk factors of blood infection in Chinese citizens' organ donation,provide the basic evidence for early protection,increase the success rate of donor distribution,and expand the Chinese organ donation pool.Methods A retrospective study was performed on 70 cases of donation recruited during October 2014 to January 2016.The incidence of blood infection in these donors was analyzed.The univariate analysis and multivariate logistic regression analysis were used to find out the high risk factors influencing the donor blood infection.Finally,the donor blood infection assessment model and the receiver operating characteristic (ROC) curve were established to assess the sensitivity and specificity.Results The overall infection rate was 64.3％ (45/70).The pulmonary,blood,and urinary tract infection rate was 42.9％,31.4％ and 1.4％ respectively.The total length of hospital stay (＞10 days) (P =0.017),oxygenation index (＜ 233.5 ± 107.0) (P =0.046),aspartate aminotransferase (＞196.9 ± 329.1 U/L) (P =0.044),and valley alanine aminotransferase (＞95.0 ± 78.1 U/L) (P =0.026) were four risk factors for predicting the donor blood infection.The multivariate logistic regression analysis revealed that the total length of stay ＞10 days along with the donors' oxygenation index (＜233.5 ± 107.0) was independent risk factor for predicting the blood infection.The donor blood infection model was:0.193 + 1.753 hospital stay (＞10 days)-0.007 oxygenation index.The sensitivity and specificity were 0.682 and 0.75 (P ＜0.001) respectively.Conclusion For a long-term stay in ICU,the rate of blood infection for donors was much higher,at this time,the most effective antibiotics should be chosen.Besides,improving donor oxygenation index and liver function can reduce the incidence of infection.

Objective To summarize our experience in hepatic artery reconstruction in adult-to-adult right lobe living donor liver transplantation(LDLT).Methods A retrospective analysis was made for 17 cases undergoing LDLT in our center from May 2007 to Oct 2008.Results All the 17 right lobe graft of the liver was supplied by single right hepatic artery and the mean diameter of right hepatic artery was 3.1 mm.The hepatic artery for segment 4 was mainly originated from left hepatic artery(12/17,70.1%).The recipient right or left hepatic artery was used in 14 cases of reconstruction,proper hepatic artery was used in 2 cases,and gastroduodenal artery was used in one case.Anastomosis was performed with interrupted 8-0 prolene and 12-16 stitches were made on the posterior wall first and then the anterior wall to avoid turning over the vessel.The mean anastomosis time was(51±26) minutes and all hepatic arteries were patent immediately after anastomosis.Hepatic arterial complications including hepatic artery thrombosis (HAT)did not occur after LDLT.Conclusions Detailed evaluation and careful protection of the hepatic artery of segment 4 are the key to successful reconstruction of hepatic artery in LDLT.Anastomosis was performed without flipping the artery wall helped to reduce the difficulty of operation remarkably and with a good result.

Objective To explore the relationship between hepatitis B virus (HBV) concentration and hepatocellular carcinoma (HCC) recurrence in HCC patients undergoing orthotopic liver transplantation (OLT). Methods 148 HCC patients associated with HBV infection undergoing OLT were enrolled in the study.Survival analysis was performed using the Kaplan-Meier method.Cox multiple regression analysis was performed to determine the parameters predicting HCC recurrence. Results Survival rates at 1,3 and 5 years were 86%,72% and 72%,respectively,and disease-free survival rate were 79%,71% and 54%,respectively.In this series 43 patients suffered from HCC recurrence.The recurrence rate was 29.1%(43/148),with the mean recurrence time being (13.16 ± 14.17) months (1 ～ 54 months).Exceeding Milan criteria (HR = 9.89; 95% CI 2.30 ～ 42.52; P = 0.002) and pretransplant HBV DNA level ＞ 5log10copies/ml (HR = 2.26; 95% CI 1.01 ～ 5.04; P = 0.047) were significant independent predictors for posttransplant HCC recurrence. Conclusion High HBV DNA load before transplantation is statistically associated with recurrence of HCC after liver transplantation.