Objective To study the feasibility of cerebral perfusion weighted imaging(PWI)using superparamagnetic iron oxide particles(SPIO)Resovist in normal rabbits,and investigate the methodology and the proper contrast agent dose of SPIO in PWI.Methods All 30 rabbits were randomly divide into 5 groups(n=6 for each),group A,B,C,D were divided according to the different dose of Resovist(4,8,16 and 32 μmol Fe/kg)and group E was operated as control study with 0.2 mmol/kg Gd-DTPA.The dynamic MR perfusion imaging series were acquired in each group.The signal intensity time curves were analyzed in gray matter and white matter,and the parameters(rCBV,SRRmax,QrCBV,QSRR max)were calculated and compared in all groups and correlated with control one.Results a bolus injection was succeed for all studies.and a dose-dependent strong reduction in signal intensity of gray and white matter in all groups was demonstrated.The rCBV values of gray and white matter for group A,B,C,D and E were(50.48±3.84),(25.57±2.10);(94.69±2.60),(45.33±3.14);(141.13±6.26),(67.67±4.65);(243.75±5.90),(162.06±5.14);and(84.60±3.60),(41.36±2.18)ml/100 g,respectively.The SRRmax values of gray and white matter for group A,B,C,D and E were(13.70±1.50)%,(7.38±0.41)%;(31.01±4.06)%,(16.49±2.35)%;(43.81±3.42)%,(21.64±4.14)%;(64.49±5.35)%,(43.61±5.78)%;and(27.78±2.98)%,(14.42±2.25)%;respectively.The QrCBV values for group A,B,C,D and E were 1.98±0.07,2.09±0.11,2.09±0.07,1.50±0.01,and 2.05±0.03,respectively,and the QSRR max values for group A,B,C,D and E were 1.85±0.11,1.88±0.06,2.06±0.25,1.49±0.09,and 1.94±0.12,respectively.All above values among group were significantly different(P＜0.01).For the QrCBV and QSRR max values between group,there were no significant difference between group A and B,B and C,A and C;while the values of group A was significantly larger than those of group D(P＜0.01).Condusion Resovist(SPIO)can be used in the study of MR perfusion weighted imaging in the brain,and the proper contrast agent dose is 4-16 μmol Fe/kg.

Objective To understand the drug resistance status of extended-spectrum beta-lactamase(ESBLs)-producing Esche-richia coli (E.coli)and Klebsiella pneumonia (KPN ),and the prevalence situation of TEM and SHV genotype in Wusu city.Meth-ods E.coli and KPN isolated in two hospitals of Wusu city from July 2011 to June 2013 were collected and the drug sensitivity test was performed by the disk diffusion method (K-B method),the ESBLs preliminary screening and the phenotypic confirmatory test were carried according to the guideline of CLSI,and at the same time DNA of ESBLs producing strains was extracted and the TEM and SHV genes among them were tested by PCR and the electrophoresis method.Results Totally 221 strains of E.coli and 153 strains of KPN were collected,among them ESBLs producing strains were 37 strains and 43 strains respectively,and the detec-tion rate of ESBLs in KPN was higher than that in E.coli(χ2 =6.942,P <0.01).The drug resistance of two kinds of bacteria were similar,the resistance rate of ESBLs producing strains to beta lactam antibacterial drugs was obviously higher than that in non-en-zyme producing strains,which was mostly more than 90%;the resistance rate to penicillin and the first or second generation cepha-losporins was almost 100%;which to gentamicin and ciprofloxacin was above 50%;while which to carbapenem was lower,less than 5%.The detection rates of TEM and SHV genes were 72.9% and 54% in ESBLs producing E.coli and 81.4% and 65.1% in KPN respectively,the detection rates were basically consistent without statistically significant differences between them (P >0.05).Conclusion The detection rate of ESBLs producing E.coli and KPN in Wusu city is higher,the drug resistance situation is severe,and TEM,SHV are the important genotypes of ESBLs.

Remote ischemic postconditioning can induce cerebral ischemic tolerance and have neuroprotective effects.It has provided a novel strategy for the treatment of ischemic cerebrovascular disease.In recent years,the mechanism research of remote ischemic postconditioning and clinical applications have made significant progress and have shown encouraging prospects of clinical transformation.

Objective To develop a portable, easily-used and effective fracture fixation material during emergency treatment of battle wound. Methods Based on vacuum plasticity, macromolecule particles were filled into prefabricated bag, in which the air was deflated. The bag became stiff. When it was bound with belt, it acted as fracture fixation. Results Experiments showed that this kind of splint attached closely with body without any accessorial material. The effect was good without any restriction from region or environment. Conclusion This kind of fracture fixation splint has advantages in operation time, applicable parts and fixation effects compared with other fixation material.

Objective To analyze CT findings of solitary fibrous tumors of the pleura (SFTP) for differentiating benign and malignant lesions.Methods CT findings of 20 benign and 11 malignant SFTPs proved by pathology were retrospectively reviewed. The size,margin,internal structures,blood supplies,invasion to adjacent structures or metastasis and pleural effusion were analyzed and compared between malignant and benign groups.Results The mean diameter of malignant lesions( median diameter:13.5 cm) was larger than that of the benign ( median diameter:7.6 cm,F =6.411,P =0.017 ).Malignant lesions tended to be more heterogeneous (9/11,P =0.002),more invasive to adjacent structures ( 6/11,P =0.001 ) than benign lesions ( 4/20,0/20 ).Serpiginous vessels ( 10/11,P =0.008 ) and pleural effusion (6/11,P =0.038 )were more common in malignant lesions than those in benign lesions (8/20,3/20).Calcification could be found in both benign (2/20) and malignant lesions ( 2/11,P =0.6 ).Furthermore,3D CT angiography could show blood supplies into tumor in 12 lesions (4 benign and 8 malignant SFTPs).Conclusion CT and 3D CTA are helpful in differentiating benign from malignant SFTPs and evaluating the excision of SFTPs.

According to relevant national laws and regulations, practitioner training was included into laboratory animal science teaching reform.By adjusting the training content and teaching method and use of animal models of typical human diseases, the transformation of training mode was realized and improved.By the assessment of basic theory in combination with practical operation, the thinking ability and hands-on skill of the practitioners are much improved. Through classroom instruction, experimental teaching, quality assessment and tracking survey, the evaluating process of the training quality of training teaching is performed.Therefore, the teaching reform of the laboratory animal science based on the training of practitioners is established.

Objective To evaluate the predictive value of procalcitonin (PCT),C-reactive protein (CRP)and white blood cell (WBC)for the severity of community-acquired pneumonia(CAP)in adult and elderly patients.Methods A total of 1 12 hospitalized patients with community acquired pneumonia from May 2013 to September 2014 were selected,and they were divided into the adult group(n=50)and the elderly group (n =62).The PCT,CRP and WBC levels in patients with severe and non-severe pneumonia were compared,and the correlation between those inflammatory markers and pneumonia severity index (PSI),British Thoracic Soci-ety CURB-65 Score(CRUB-65)were analyzed in each age group.Results PCT and CRP levels,PSI and CURB-65 scores in adult and elderly patients with severe pneumonia were higher than those in patients with non-severe pneumonia of corresponding age group (P < 0.05 ).Moreover,PCT had significant positive correlation with PSI and CURB-65 scores in the elderly group (P <0.05).But no correlation were found between the two kinds of scores and PCT in adult group as well as CRP,WBC in the two age group(P >0.05).Conclusion PCT and CRP might be useful markers to evaluate the severity of CAP,and PCT has a higher pre-dictive value in elderly patients with CAP.

OBJECTIVE:To explore the clinical efficacy and safety of cetuximab combined with NP regimen and radiotherapy in the treatment of advanced non-small cell lung cancer(NSCLC). METHODS:76 cases of advanced NSCLC were selected and randomly divided into control group and observation group according to different therapy methods,with 38 cases in each group. Control group received NP regimen(cisplatin 25 mg/m2+navelbine 12.5 mg/m2)+thoracic IMRT;observation group was additional-ly given cetuximab 400 mg/m2(first day),with maintenance dose of 250 mg/m2 weekly and last for 13 weeks. Short-term efficacy, survival situation were compared between 2 groups as well as the levels of T lymphocyte,Th1,Th2,immuneglobulin(Ig) and complement. The occurrence of ADR was recorded. RESULTS:The total effective rate of observation group was 86.84%,which was significantly higher than that of control group(65.79%),with statistical significance(P<0.05). Average survival period and 2-year survival rate of observation group were 18.70 months and 55.26%,which were significantly longer or higher than those of control group (14.75 months,31.58%),with statistical significance (P<0.05). The improvement rate of survival quality was 94.74% in observation group,which was significantly higher than 68.42% in control group,with statistical significance (P<0.05). After treatment,the levels of CD4+,CD4+/CD8+ and IgG were significantly increased in 2 groups,while the levels of CD8+, Th1,Th2 and Th1/Th2 were significantly decreased;the observation group was better than the control group,with statistical signifi-cance(P<0.05);there was no statistical significance in the levels of CD3+,IgA,IgM and complement 4 between 2 groups(P>0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Cetuximab combined with NP regimen and radiotherapy can improve clinical efficacy of advanced NSCLC,improve survival quality,prolong survival time and promote the recovery of Ig,complement and T lymphocyte,with good safety.

Objective To investigate the best operative approach in the treatment of myasthenia gravis (MG) by comparing surgical effects of median sternotomy, trans-sternal surgery and video-assisted thoracoscope surgery (VATS). Methods One hundred and nine patients who received thymectomy for MG were divided into median sternotomy group,trans-sternal group and VATS group according to the way of operative incision. The clinical features, such as operative time, operative blood loss, postoperative drainage volume and drainage time, postoperative hospitalization time, postoperative pulmonary infection, incisional infection, MG crisis, postoperative chronic pain and upper limb's movement disorder were retrospectively analyzed to evaluate the effect of the surgery. Results The operative blood loss, postoperative drainage volume and drainage time,and incisional infection in VATS group [(77.5 ± 18.0) ml, ( 100.8 ± 11.8) ml,( 2.3 ± 0.5 ) d, 0 ] were superior to those in trans-sternal group[ (97.4 ± 14.3 ) ml, ( 175.8 ± 18.3 ) ml, ( 3.1 ±0.7 ) d, 6.2% ( 2/32 ) ] and median stemotomy group [ ( 130.1 ± 24.0) ml, ( 379.0 ± 45.6 ) ml, (4.2 ± 0.6) d,13.8% (9/65)] (P ＜0.05),and median sternotomy group was the worst. The incidence of postoperative pulmonary infection, MG crisis, upper limb's movement disorder and postoperative hospitalization time in VATS group were superior to those in trans-sternal group and median sternotomy group (P ＜0.05).Conclusions Three mini-incision surgical approaches are safe, feasible and effective. VATS is more advantageous and is worth popularizing in large and medium-sized hospitals. Trans-sternal surgery is the better choice for primary hospitals without advanced technology and equipments.

Ocean is a unique and excellent resource that provides a diverse array of intriguing natural products. Marine natural products have demonstrated significant and extremely potent biological activities and have captured the attention of natural products chemists in the past few decades. It is increasingly recognized that a wealth of fascinating natural products and novel chemical entities will play a dominant role in the discovery of useful leads for the development of pharmaceutical agents and provide useful probes to lead to breakthroughs in a variety of life-science fields. This article focused on the research progress of chemistry of marine natural products in recent five years.