The clinical data of 21 cases of adrenal ganglioneuroma treated in our hospital from July 1988 to August 2008 were reviewed. The clinical manifestations were: lower back uncomfortableness (9 cases), paroxysmal dizziness (4 cases), central obesity ( 1 case) and 7 cases were discovered incidentally.Five cases were diagnosed pre-operationally and remaining 16 cases were diagnosed post-operationally by pathology. All patients completely recovered after surgery except one, who still had dizziness with high blood pressure. Ultrasonography, CT and MRI examinations are the main diagnostic methods for adrenal ganglioneuroma, and it is confirmed by histopathology, surgical removal of the tumor is the main therapy for the disease.

Objective To measure the levels of neutrophil elastase (NE),citric acid and pH in prostatic fluid of patients with prostatitis type Ⅲ.Methods Fifty two patients with prostatitis type ⅢA,98 patients with prostatitis type Ⅲ B and 105 health subjects were enrolled in the study.The levels of NE,citric acid and pH in prostatic fluid were measured ; the expressed prostatic secretion (EPS) routine and bacterial culture were examined,and National Institutes of Health (NIH)-chronic prostatitis symptom index (NIH-CPSI) was evaluated.The data were analyzed by independent samples t-test.Results WBC count,NE and citric acid concentrations,pH in ⅢA group were (17.9 ±5.4)/HP,(898 ±704) μg/L and (14.5 ± 1.7) ng/L,7.60 ±0.43,respectively; those in ⅢB group were (3.9 ±2.2)/HP,(93 ±76) μg/L and (21.9 ±3.8) ng/L,6.71 ±0.25,respectively; those in control group were (3.6 ±2.2)/HP,(86 ±57) μg/L and (22.5 ± 3.9)ng/L,6.48 ± 0.51,respectively.There were significant differences in NE and citrate concentrations,pH value in EPS between Ⅲ A and Ⅲ B patients (t =8.22,16.64 and 13.88,all P ＜0.05),but no difference in NIH-CPSI (t =1.90,P 8 0.05).There were significant differences in WBC count,NE and citric acid concentrations,pH in EPS,NIH-CPSI score between Ⅲ A and control groups (t =18.92,8.47,26.53,18.37 and 32.47,all P ＜ 0.05).There were no differences in WBC count,NE and citric acid concentration and pH value in EPS between Ⅲ B group and control group (t =1.38,1.55,1.02 and 1.21,respectively,all P 80.05),but there was significant difference in NIH-CPSI score between two groups (t =49.46,P ＜ 0.05).In EPS,NE concentration and WBC count were positively correlated with NIH-CPSI (r =0.819 and 0.698,respectively,all P ＜0.01),and citric acid was negatively correlated (r =-0.625,P ＜ 0.01) ; citrate was negatively correlated with WBC count,CPSI and pH value (r =-0.728,-0.644 and-0.817,all P ＜ 0.01).Conclusions The results indicate that the measurements of NE,citric acid and pH in EPS are of significant clinical value in patients with prostatitis type ⅢA and Ⅲ B.

Two hundred and five patients with a confirmed diagnosis of aldosterone-producing adenomas underwent retroperitoneal laparoscopic operation from January 1999 to December 2009 in our hospital. All cases had hypokalemia and hypertension preoperatively. Of all the cases, 80 received total adrenalectomy and 125 received partial adrenalectomy. Operations were successfully performed in all cases. The time of operation ranged from 15 to 150 min [mean(45 ±13) min] and the volume of bleeding during operation ranged from 10 to 200 ml [mean(25 ±10) ml]. The length of hospital stay ranged from 4 to 9 d [mean(6. 8 ± 1. 2) d]. All patients were followed up for 0. 5 - 2 years (mean 1. 2 years). After operations blood potassium levels returned to normal in all cases, blood pressure returned to normal in 168 cases (82.0% ) . Results indicated that partial or total adrenalectomy with retroperitoneal laparoscopy for aldosterone-producing adenomas was safe and feasible technique.

[Objective] To observe the clinical effect of TCM diet therapy on type-2 diabetes.[Method] Select the said patients 76 cases,randomly divide them into treatment group and control group with 38 cases in each group,which were administered with routine western medicine,while the treatment one was added with TCM diet medication.[Result] Compare the fasting glucose before medication,showing marked difference(P

Prostate cancer is the most common malignant tumor in men of western countries and its incidence is increasing in China. However, the traditional methods of screening and diagnosing of prostate cancer are of limited value. Multiparametric magnetic resonance imaging (MRI) is an examination which combines morphologic sequences with one or more functional sequences. Multiparametric MRI can not only display the anatomical structures and morphologic changes of organs, but also reflect some histological components, providing pathophysiological and biochemical information of tissues and guiding prostate targeted biopsy at the same time. These advantages give multiparametric MRI high application value in the diagnosis and evaluation of prostate diseases. This review summarized the recent progress in multiparametric MRI diagnosis of prostate cancer.

Objective To investigate the antitumor effect of PRDM5 gene in prostate cancer cells. Methods PRDM5 gene was cloned and inserted into lentiviral vector using polymerase chain reaction (PCR), restriction endonuclease and T4 DNA ligase connected method. The lentiviral plasmids carrying PRDM5 gene (LV-PRDM5) or control lentivirus (LV-Luc) were co-transfected with lentiviral packaging plasmid mix into 293T cells by liposome method. The viral supernatants were collected and transduced into human prostate cancer cells 22Rv1. The expression of PRDM5 was verified by Western blotting analysis. The cell proliferation and clone formation ability were detected by cell multiplication and cell cloning experiments. The anchorage independent growth rate of prostate cancer cells was assessed by soft agar colony formation assay. Results The lentivirus vector expressing PRDM5 gene was constructed successfully, and the viral supernatants were obtained. The prostate cancer cell line 22Rv1 stably expressing exogenous PRDM5 was screened and verified by Western blotting analysis. Compared with control cells, the prostate cancer cell line 22Rv1 expressing PRDM5 showed a lower growth rate (multiplication time: [52.5±1.4] vs [44.0±1.3] h), clone formation rate ([1 114±98] vs [1 361±123] colonies per dish) and anchorage independent growth rate ([94.6±8.7] vs [154.0±3.5] colonies per cell, P<0.05). Conclusion Overexpression of PRDM5 has inhibitory effect against proliferation, clone formation and anchorage independent growth of prostate cancer cells in vitro.

Objective To evaluate the safety and efficacy of transcatheter closure of renal artery with a newly-developed double waist occluder in dogs. Methods A newly-developed double waist occluder was implanted in the right renal arteries of 12 healthy dogs. The location of devices was observed by angiography immediately, and 1, 3 and 6 months after occlusion. Renal specimens and occluders was observed after the animals were sacrificed and pathological examination was conducted. Also the endothelialization on the surface of the occluders was observed. Results Transcatheter closure of unilateral renal artery was successfully performed in all the 12 dogs. One dog died due to overdose of narcotic drugs, and the other 11 dogs survived well. Complete occlusion of the renal artery was achieved in 6 dogs immediately after the occluder was implanted, and complete occlusion was seen in all the dogs within 12 min after occlusion, with all the devices in good position and without translocation. Repeated angiography showed in all animals that the occluders were in good position and without residual shunt. The occluded kidneys became smaller than the opposite ones, with the surface of occluded kidney turning lumpy and hard. Endocardial cells started to cover the surface of occluder 1 month after closure and complete coverage was seen 3 months later. Conclusion The newly-developed double waist occluder is safe, feasible and effective, and it can be delivered in small sheath owning to its excellent trafficability.

Objective To evaluate the safety and efficacy of laparoscopic partial nephrectomy via a combined "trans-retro-peritoneal three-step" approach. Methods The clinical data of 32 patients who received laparoscopic partial nephrectomy between Jul. 2015 and Nov. 2015 were retrospectively analyzed. Twenty cases were via the traditional transperitoneal approach and the other 12 cases via a novel combined "trans-retro-peritoneal three-step" approach. The 3 steps in this novel approach included: (1) Through transperitoneal approach, Gerota's fascia and perirenal fat were incised to expose the tumor; (2) The peritoneum and Gerota's fascia were incised along the Told's line, and the renal artery was then separated on the surface of the psoas muscle; and (3) The renal artery was clamped, the edge of the tumor was marked, and finally the tumor was resected and the kidney was reconstructed. The perioperative data of the two groups were compared. Results The surgeries were successfully performed in all the 32 patients. Compared to traditional transperitoneal approach, our novel approach significantly shortened the operation time (P=0.014). The other perioperative parameters, such as estimated blood loss, were comparable in the two groups. Conclusion This combined "trans-retro-peritoneal three-step" approach has the combined the advantage of traditional transperitoneal (enough room for manipulation) and retroperitoneal (convenience to separate the renal arteries) approaches, especially when the renal artery is abnormal. Further studies are needed to compare the present approach with the traditional surgeries.

Objective To investigate the efficacy and safety of robot-assisted partialnephrectomy (RAPN) by summarizing the surgical experience Methods The clinical data of 230 patients who underwent RAPN ln ChanghalHospital from March 2012 to June 2015 were analyzed. The patients included 161males and 69 females, with an average age of (51. 1± 12. 1) years old. There were 111 tumors ln the lett kidney and 118 in the right, with 1 case having tumors in bilateral kidneys. There were 13 hilar tumors and 8 endophytic tumors. There were 54 large tumors (>4 cm in diameter) and 3 tumors in solitary kidney. Preoperative glomerular filtration rate (GFR) test was normal in all cases. Kidney CTA scan showed single branch of the renal artery in 211 cases, two branches in 16 cases, and three branches in 3 cases. Results The surgery was successfully completed in all the 230 cases. A total of 198 cases were via transperitoneal approach, including 191 cases with medial camera port placement, 6 with lateral camera port placement and 1 with mix port placement; and 32 cases were via retroperitoneal approach. The mean surgery duration was (196±59) min and the mean blood loss was (148. 3± 126. 5) mL, despite 2 cases having a blood loss of more than 1 000 mL. There were 3 intraoperative blood transfusion cases and one conversion to open surgery. The mean warm ischemia time was (20. 7 ±9. 1) min. Themean postoperative hospital stay was (8. 9 ±3. 3) days. Serious gross hematuria occurred in 3 patients after the surgery, which was relieved by conservative treatment in one patient andDSA super-selective bleeding artery embolization in 2 patients. Urinary leakage occurred in one case and was cured by complete drainage. Cerebral embolism occurred in one case. Postoperative pathology reported malignant tumor in 195 cases, including renal clear cell carcinoma in 179 cases, chromophobe cell carcinoma in 11 cases and papillary renal cell carcinoma in 5 cases. T1N0M0 tumor was found in 154 cases and T2aN0M0 tumor was found in 41 cases; angiomyolipoma and other benign tumors were found in 35 cases. The maximum diameter of the tumor ranged from 1. 3 to 9. 0 cm, averaging (3. 41 + 1. 57) cm. There were no positive surgery margins in our cohort Conclusion RAPN is a safe and effective surgery for local renal tumors, and it has significant advantages in complete resection of the renal tumors and the reconstruction of the kidney.

Objective To compare the effectiveness between doubled dose rosuvastatin and rosuvastatin combined with probucol for treatment of patients with coronary artery disease and hypercholesterolemia. Methods From Jan. 2012 to Dec. 2013, 68 patients with coronary heart disease, who were treated with rosuvastatin (10 mg qn) for more than 3 months in the Cardiology Clinic, Changhai Hospital and whose low density lipoprotein cholesterol (LDL-C) still not reached the target level, were evenly randomized into two groups: the double dose statin group (rosuvastatin 20 mg qn) and the combined treatment group (rosuvastatin 10 mg qn plus probucol 250 mg bid); the treatment lasted for 3 months. The peripheral blood lipid levels and incidence rates of adverse reactions were compared between the two groups. Results The baseline data and the lipid levels were not significantly different between the two groups before treatment (P>0.05). After 3 months of treatment, the total cholestrol (TC) and LDL-C were significantly decreased in the two groups (P<0.05); triglyceride (TG) was significantly decreased only in the combined treatment group (P<0.05); the high-density lipoprotein cholesterol (HDL-C) had an increasing trend in the double rosuvastatin group and a decreasing one in the combined treatment group, but without statistical significance (P>0.05). Compared with the double rosuvastatin group, the combined treatment group had significantly greater decreases of TC and LDL-C (P<0.05), a significantly higher control rate of LDL-C (P<0.05), and a significantly lower HDL-C level (P<0.05). The combination treatment group had significantly less adverse reactions compared with the double rosuvastatin group (P<0.05). Conclusion For patients with coronary artery disease and hypercholesterolemia, it is more effective and safe to be treated with rosuvastatin combined with probucol than doubling the dose of rosuvastatin.