Objective To compare the effect of different partition of nucleic acid protein complex on the affinity of enriched library in systematic evolution of ligands by exponential enrichment (SELEX). Methods Two protocols were adopted to select the enriched library to transforming growth factor ? receptor Ⅱ(TGF ? RⅡ). Protocol 1: protein was absorbed on the surface of 96 well plate; then, selection was carried out; the binding nucleotide acids were eluted from the supporter directly. Protocol 2: selection was done in solution; nucleotide acid protein complex was captured in nitrocellulose membrane; the binding nucleotide acids were obtained from membrane. Filter biding assay and gel shift assay were performed to detect the affinity of the enriched ssDNA library from different protocols. Results After 4 rounds of selection, the affinity to TGF ? RⅡ was obviously improved in the enriched library from protocol 2 compared with the initial library, while no such improvement was found in the enriched library from protocol 1. Conclusion In the SELEX experiment, the way of selection in solution, then partition of the binding nucleotide acids in filter is easier to enrich the binding fragment from initial ssDNA random library, compared with the way of fixation of target protein to solid supporter, then selection between the solid phase and liquid phase and elution of the binding nucleotide acids from supporter.

In this study,IL-4 and IL-12 levels in plasma and peripheral blood mononuclear cells(PBMC) as well as serum IgE level were prospectively assessed with double-antibody sandwich ELISA technique in children with asthma during the attack group (30 cases)and the interval group(12 cases).The results observed revealed that serum IgE level and IL-4 level in plasma and PBMC after PHA and LPS provocation during both of the attack stage and the interval stage were found to be evidently higher than those of the normal control group (P＜0.01).Otherwise,IL-12 level during both stages was much lower than that of the normal control(P＜0.01).In other hand,there were found to have a significant difference in all these 3 data between attack and interval stages.Thus, the conclusion indicates that there might be an imbalance of IL-4,IL-12 and IgE level in children with asthma during both of the attack and the interval stages.

AIM and METHODS: To study the immunological effect of measles vaccine therapy on asthmatic children, we examined the changes of interleukin-12 , interleukin-13 and total serum IgE levels in plasma and cultured peripheral blood mononuclear cells(PBMC) supernatant by means of ELISA in 13 mild-moderate asthmatic children treated with measles vaccine. Results were compared with 12 anti-symptomatic treatment mild-moderate asthmatic children and 17 normal children control group. RESULTS:After measles vaccine treatment, IL-13 and total serum IgE levels decreased remarkably, statistically lower than that of group receiving only anti-symptomatic treatment. There was no statistical difference in IL-12 level between the two group. Correlation analysis: 1)IL-12 level of plasma was negatively correlated to the level of serum total IgE, there was no correlation of supernatant IL-12 in PBMC to the total serum IgE; 2)IL-13 levels in plasma and PBMC were positively correlated to the level of total serum IgE; 3) IL-12 level was negatively correlated to IL-13. CONCLUSION: Measles vaccine could down-regulate IL-13 level, therefore decrease total IgE synthesis, but not affect IL-12 level in asthmatic children.

Objective To explore the clinical efficacy of mifepristone combined with rivanol in induction of labor.Methods 100 patients requiring induction of labor were randomly divided in-to experimental group and control group with 50 patients in each group.The control group was giv-en intra-amniotic injection of 75 mg rivanol,while the patients in the experimental group orally took 75 mg mifepristone under fasting condition based on the therapy in the control group,and took another 75 mg after 24 hours.The score of cervical Bishop,onset time of uterus contraction,time to eliminate the fetus,total stage of labor,abortion effect,amount of postpartum hemorrhage and side effects were all observed in the two groups.Results After administration of the drugs,the score of cervical Bishop in the experimental group was markedly higher than that in the control group,while onset time of contraction,time to eliminate the fetus,total stage of labor and amount of postpartum hemorrhage were obviously less than that in the control group.The response rate of induction of labor in experimental group (1 0 0 % )was higher than that in the control group (92.0%),the difference was statistically significant.Conclusion Application of mifepristone com-bined with rivanol to terminate the pregnancy can effectively soften the cervix,shorten the time of delivery of baby,reduce patients′pain and relevant complications and ameliorate postpartum recov-ery,so it is worthy of clinically popularization.

Objective To explore the clinical efficacy of mifepristone combined with rivanol in induction of labor.Methods 100 patients requiring induction of labor were randomly divided in-to experimental group and control group with 50 patients in each group.The control group was giv-en intra-amniotic injection of 75 mg rivanol,while the patients in the experimental group orally took 75 mg mifepristone under fasting condition based on the therapy in the control group,and took another 75 mg after 24 hours.The score of cervical Bishop,onset time of uterus contraction,time to eliminate the fetus,total stage of labor,abortion effect,amount of postpartum hemorrhage and side effects were all observed in the two groups.Results After administration of the drugs,the score of cervical Bishop in the experimental group was markedly higher than that in the control group,while onset time of contraction,time to eliminate the fetus,total stage of labor and amount of postpartum hemorrhage were obviously less than that in the control group.The response rate of induction of labor in experimental group (1 0 0 % )was higher than that in the control group (92.0%),the difference was statistically significant.Conclusion Application of mifepristone com-bined with rivanol to terminate the pregnancy can effectively soften the cervix,shorten the time of delivery of baby,reduce patients′pain and relevant complications and ameliorate postpartum recov-ery,so it is worthy of clinically popularization.

Aim To explore the influencing factors of major adverse cardiovascular events(MACE)after drug-e-luting stent(DES)implantation in young and middle-aged patients with coronary artery disease(CAD).Methods Retrospective study data from the First Affiliated Hospital of Zhengzhou University were extracted from the Dryad database,and young and middle-aged CAD patients who received DES were divided into MACE group(n=57)and non MACE group(n=321)according to whether MACE occurred during the follow-up period.Clinical data including age,gender etc.were compared between the two groups,and the variables with significant differences between the two groups were substitu-ted into Logistic regression model to screen the influencing factors of MACE after DES implantation in young and middle-aged CAD patients.Value of influencing factors predicting occurrence of MACE after DES implantation in young and mid-dle-aged CAD patients was evaluated by plotting ROC curve and calculating the area under the curve(AUC).Results Stent diameter in MACE group was significantly smaller than that in non MACE group(P＜0.001),the number of left main coronary artery lesion in MACE group was significantly higher than that in non MACE group(P＜0.05).Multivariate Lo-gistic regression analysis indicated that stent diameter(OR=0.184,95％CI:0.084～0.405,P＜0.001)and left main coronary artery lesion(OR=9.319,95％CI:2.291～37.904,P=0.002)were the independent risk factors of MACE after DES implantation in young and middle-aged CAD patients.The risk of MACE after DES implantation in young and middle-aged CAD patients significantly decreased when the stent diameter was＞3 mm.The AUC of stent diameter com-bined with left main coronary artery lesion predicting occurrence of MACE after DES implantation in young and middle-aged CAD patients was 0.700(95％CI:0.623～0.776).Conclusion The smaller stent diameter and left main coronary artery lesion are the influencing factors of the occurrence of MACE after DES implantation in young and middle-aged pa-tients with CAD,and should be emphasized by clinical practitioners.

Hemangioma of the penile head is rare. This paper reported a patient, 16 years old, who was admitted to hospital due to the discovery of multiple masses on the head of the penis for more than two years. Physical examination showed that three vascular mass-like masses were distributed along the coronal sulcus at the 3, 9, and 12 points of the penile head, and the larger one was about 10 mm×5 mm size, blue-purple, soft, and painless. Ultrasound examination suggested that the patient had a penile head hemangioma. Surgical resection was performed, and the postoperative pathological diagnosis was penile head hemangioma.The follow-up of 3 months showed that the wound healed well without recurrence, and the penile head appearance was not obviously deform.