AIM: To establish the methods for determining borneol,isoborneol,muscone,benzyl benzoate in Shexiang Baoxin Pills. METHODS: Turbomass GC-MS instrument was used;the selective ion was applied for detecting borneol,isoborneol,muscone,benzyl benzoate.DB-5 capillary column(0.1 ?m?0.25 mm?30 m).The column temperature was rose by program from 80 ℃ to 260 ℃;the quantiative ion m/z 95 used for borneol and isoborneal,m/z 238 for musone,m/z 212 for benzyl benzoate;Shexiang Baoxin Pills were diluted with hexane. RESULTS: The linear ranges of borneol,isoborbeol,muscone,benzyl benzoate were within 0.020-20.0 ?g/mL(r=0.999 3),0.024-24.0 ?g/mL(r=0.999 0),0.029-28.8 ?g/mL(r=0.999 1),0.050-50.0 ?g/mL(r=(0.999 2)) respectively.The average recoveries of borneol,isoborbeol,muscone,benzyl benzoate were 97.00%,(96.30%,) 96.85%,98.20%,respectively. CONCLUSION: The method is simple accurate and highly sensitive and suitable for Shexiang Baoxin Pills.

Objective To comparison of the effect of tigrine and clopidogrel in PCI in patients with STEMI .Methods 118 cases of routine PCI in patients with acute ST segment elevation myocardial infarction from March 2014 to May 2014 in our hospital were select, according to random number table method to all of the patients were randomly divided into clopidogrel group (group A) and tigrine group(group B), preoperative group A received 300 mg of aspirin combined with 600 mg of clopidogrel for treatment,group B received 300 mg aspirin combined with 180g of tigrolipine for preoperative treatment.Compared the two groups of patients with intraoperative infarct-related artery myocardial infarction thrombolytic test three blood flow and 1.5 hours after ST segment fall, postoperative heart B in the situation, the incidence of dyspnea, adverse cardiac events, bleeding events.Results The incidence of ST segment depression and the incidence of ST segment depression in group B were significantly higher than those in group A (t=12.234,χ2 =7.829,P<0.05).One month after surgery, B group of patients with LVEF value was significantly higher than the A group (t=5.393,P<0.05);there was no significant difference in LVDD between the two groups.The incidence of dyspnea and immediate TIMI grade 3 blood flow in group B were 24.6% and 88.5%, respectively, which were significantly higher than those in group A and 8.8% and 68.4% (χ2 =4.293,χ2 =4.392,P<0.05). The incidence of adverse cardiovascular events was 19.3% in group A, which was significantly higher than that in group B (χ2 =2.193,P<0.05). There was no significant difference in the incidence of bleeding between the two groups.Conclusion The use of STEMI patients underwent PCI surgery ticagrelor treatment, in case of the risk of bleeding can effectively improve the patient's myocardial reperfusion and prognosis does not increase , the increased incidence of immediate thrombolysis in myocardial infarction patients in the three grade .

Objective: To construct the expressing vector of the extracellular domain of death receptor 5 (DR5), express it E.coli, identify the purified DR5 protein, and study its biological activity. Methods: The extracellular domain of DR5(eDR5) was assembled by overlapping PCR. The expression vector pET-22b(+)/ DR5 was constructed and transformed into E.coli BL21(DE3). The expression of eDR5 protein was induced by IPTG and purified by Ni 2+ -affinity chromatographic column. The purity and specificities were detected by SDS-PAGE and ELISA, respectively. The blocking effects of purified eDR5 on FMU1.5-induced apoptosis of U343, U373 cells were observed. Results: The extracellular domain of DR5 was obtained by overlapping PCR. The eDR5 protein was expressed in both supernatants and inclusion bodies with a yield more than 30% of total bacterial proteins. The purity of eDR5 was more than 95% and the yield reached 9 mg/ml. The result of ELISA showed the purified protein was eDR5. Purified eDR5 partially blocked the apoptosis of U343 cells induced by FMU1.5 and TRAIL. Conclusion: The successful construction, expression, and purification of the extracellular domain of DR5 protein lays a foundation for further study of DR5 function.

Secondary injury and the glial scar after spinal cord injury can adversely affect nerve regeneration in the microenvironment, leading to treatment difficulties. Mesenchymal stem cells (MSCs) have multi-differentiation, self-renewal ability, participation in immune regulation and other functions and can be used to treat spinal cord injury, but they are not easy to pass the blood spine barrier and have high tumorigenicity and other disadvantages. MSCs-derived exosomes are nanometer exosomes secreted by MSCs, which contain many active substances. These exosomes have a strong neurorepair effect in spinal cord injury research, and have advantages of easy penetration, stability and low tumorigenicity, etc. These exosomes make up for the disadvantages of MSCs treatment, and are expected to replace MSCs in treatment of spinal cord injury. The authors review the biological characteristics of MSCs-derived exosomes and its mechanism in the treatment of spinal cord injury, so as to provide references for the treatment of spinal cord injury.