Objective To investigate the effects of chloroquine on metabolism of insulin,glucose,lipids and expression of insulinase gene (EIG) in patients with type 2 diabetes mellitus.Methods Fasting plasma insulin (FINS),fasting plasma glucose (FPG),glycoslated hemoglobin (HbA1c),plasma lipids and lipoprotein,insulinase activity of erythrocytes (EIA) and EIG were determined in 30 patients with type 2 diabetes mellitus laking chloroquine for 14 day in dosage of 250mg twice daily,27 diabetic patients laking placebo and 20 normal subjects.Insulin sensitive index (ISI) were also calculated.Results Chloroquine caused a decrease in total plasma cholesterol,low density lipoprotein,EIA and EIG,and an increase in ISI,plasma high density lipoprotein and subclass 2.No change of these indices was observed in diabetic patients with placebo.Conclusion Chloroquine can ameliorate the dyslipidemia and insulin sensitivity of patients with type 2 diabetes mellitus.This may be due to a decrease in EIG,which may result in lowered degradation of insulin.

Objective To investigate the best weighting of adaptive statistical iterative reconstruction (ASIR) algorithm and optimized low-dose scanning parameters in thoracic aorta CT angiography(CTA).Methods Totally 120 patients with the body mass index (BMI) of 19-24 were randomly divided into 6 groups.All patients underwent thoracic aorta CTA with a GE Discovery CT 750 HD scanner (ranging from 290-330 mm).The default parameters (100 kV,240 mAs) were applied in Group 1.Reconstructions were performed with different weightings of ASIR (10％-100％ with 10％),and the signal to noise ratio (S/N) and contrast to noise ratio (C/N) of images were calculated.The images of series were evaluated by 2 independent radiologists with 5-point-scale and lastly the best weighting were revealed.Then the mAs in Group 2-6 were defined as 210,180,150,120 and 90 with the kilovoltage 100.The CTDIvoland DLP in every scan series were recorded and the effective dose (E) was calculated.The S/N and C/N were calculated and the image quality was assessed by two radiologists.Results The best weighing of ASIR was 60％ at the 100 kV,240 mAs.Under 60％ of ASIR and 100 kV,the scores of image quality from 240 mAs to 90 mAs were(4.78 ±0.30)-(3.15 ±0.23).The CTDIvol and DLP were 12.64-4.41 mGy and 331.81-128.27 mGy,and the E was 4.98-1.92 mSv.The image qualities among Group 1-5 were nor significantly different (F =5.365,P ＞ 0.05),but the CTDIvol and DLP of Group 5 were reduced by 37.0％ and 36.9％,respectively compared with Group 1.Conclusions In thoracic aorta CT Angiography,the best weighting of ASIR is 60％,and 120 mAs is the best mAs with 100 kV in patients with BMI 19-24.

AIM:To evaluate the changes of lung morphologic features and oxidative stress in experimental diabetic rats. METHODS: The pulmonary structure of alloxan-induced diabetic rats were quantitatively studied with stereological methods. Changes of ultrastructure, activities of superoxide dismutase (SOD) and contents of malondialdehyde(MDA) of diabetic lung and serum were observed. RESULTS: The volume proportion of alveolar air and mean linear intercept of diabetic rats decreased remarkably in comparison with controls while the volume proportion of alveolar wall, the surface density of alveolar, the numerical density of alveolic area, the numerical density of alveolar and specific surface of alveolar increased significantly. The major change of the type II pneumocyte of diabetic rats was dilation of the rough endoplasmic reticulum (RER). The other findings in diabetic rats had included the presence of thickened alveolar epithelial, pulmonary capillary basal laminae and blood- air barrier, the volume density, the surface density, the mean profile area and the mean perimeter of RER in type Ⅱ pneumocyte of diabetic rats increased remarkably. And the specific surface of RER was significantly lower as compared with controls. SOD activity decreased and MDA content increased significantly in serum of diabetic rats as compared with the control group. SOD activity in the diabetic lung was not different from that of the control lung. However, the content of MDA obviously increased in diabetic lung. CONCLUSION: The morphologic features and oxidative stress in early diabetic rats are abnormal thus the lung should be considered as one of the “target organ”in diabetes mellitus.

Objective:To study the origin traceability of anemarrhenae rhizoma from Bozhou and Hebei based on multi-element fingerprints technology , and establish a discrimination model .Methods:The contents of 48 elements were determined by using induc-tively coupled plasma mass spectrometry ( ICP-MS) for 44 samples of anemarrhenae rhizome from Bozhou and Hebei province .Princi-pal component analysis ( PCA) and orthogonal partial least squares discrimination analysis ( OPLS-DA) were applied in the data analy-sis to screen out the significant elements .And then Fisher linear discrimination analysis was used to determine the origin of anemarrhe-nae rhizoma and the discrimination models were developed .Results:Two discrimination models were developed by the discrimination a-nalysis of the whole model method with nine significant elements identified by PCA and OPLS -DA, and 100％correct classification and 95.5％cross validation were achieved by the models .Conclusion: It is a promising approach to classify the geographical origin of anemarrhenae rhizome based on multi-element fingerprints analysis combined with multivariate statistical analysis .The discrimination models are good enough to be applied in the origin traceability of anemarrhenae rhizome.

OBJECTIVE: To investigate the early effectiveness of transiliac-transsacral screws internal fixation assisted by augmented reality navigation system HoloSight (hereinafter referred to as "computer navigation system") in the treatment of posterior pelvic ring injuries. METHODS: A retrospective analysis was made in the 41 patients with posterior pelvic ring injuries who had been treated surgically with transiliac-transsacral screws between June 2022 and June 2023. The patients were divided into navigation group (18 cases, using computer navigation system to assist screw implantation) and freehand group (23 cases, using C-arm X-ray fluoroscopy to guide screw implantation) according to the different methods of transiliac-transsacral screws placement. There was no significant difference in gender, age, body mass index, causes of injuries, Tile classification of pelvic fracture, days from injury to operation, usage of unlocking closed reduction technique between the two groups ( P>0.05). The time of screw implantation, the fluoroscopy times, the guide wire adjustment times of each screw, and the incidence of complications were recorded and compared between the two groups. The position of the transiliac-transsacral screw was scanned by CT within 2 days after operation, and the position of the screw was classified according to Gras standard. RESULTS: The operation was successfully completed in both groups. The time of screw implantation, the fluoroscopy times, and the guide wire adjustment times of each screw in the navigation group were significantly less than those in the freehand group ( P<0.05). There were 2 cases of incision infection in the freehand group, and the incision healed by first intention after active dressing change; there was no screw-related complication in the navigation group during operation and early period after operation; the difference in incidence of complications between the two groups (8.7% vs. 0) was not significant ( P=0.495). According to the Gras standard, the screw position of the navigation group was significantly better than that of the freehand group ( P<0.05). CONCLUSION Compared with the traditional freehand method, the computer navigation system assisted transiliac-transsacral screws internal fixation in the treatment of posterior pelvic ring injuries has advantages of improving the accuracy of screw implantation and reducing radiation damage and the time of screw implantation.
Humans ; Retrospective Studies ; Surgical Wound Infection ; Replantation ; Body Mass Index