Objective To investigate the diagnosis and treatment for tuberculosis of the breast.Methods Retrospectively analyzed the clinical data of 78 patients with tuberculosis of the breast treated in our hospital in the past 21 years was made.Results The incidence of tuberculosis of the breast has been increasing year by year,peasants of remote mountain areas and civilian workers in the cities were the high affected groups.Misdiagnosis rate was as high as 62.9 %.Operation combined with other therapies,and selection of opportune time for operation can reduce the extent of resection,and satisfy the demands of young female patients for high quality of life.Conclusions Clinical doctors should improve their recognition of tuberculosis of the breast,increase the rate of correct diagnosis,and avoid missed diagnosis and misdiagnosis,especially misdiagnosis of tuberculosis for breast cancer,which can cause the patients to undergo unnecessary pain and economic burden.

Objective Voxel-based morphological analysis was used to explore the changes of gray matter volume in patients with first-episode untreated depression，and to explore the structural changes of brain regions in patients with depression，so as to provide new ideas for in-depth understanding of the pathogenesis of depression.Methods 30 patients with first-episode untreated depression and 22 healthy volunteers were included. Used the PHILPS 3.0T MRI scanner for sagittal three-dimensionl brain structre scans，the brain structure images of the two groups were studied by the VBM method to explore the changes of gray matter volume in patients with first-episode untreated depression.Results Compared to healthy controls，the volume of gray matter in left middle tempral gyrus，right inslar lobe，left supeior occiptal gyrus and right dorsolteral supeior frontal gyrus were decresed in the first-episode untreated depression group（P<0.05，cluster size>45，AlphaSim correction），no region increased.Conclusion The brain regions with decreased gray matter volume in patients with first-episode untreated depression are mainly located in frontal lobe，temporal lobe，occipital lobe and insular lobe，indicating that the occurrence and development of depression is related to multiple brain regions.

Objective　To explore the levels of peripheral blood IL-33 and the difference of arteriovenous fistula glucose (Da-jvGlu) after giving Shenqi glucose injection in acute cerebral infarction patients.Methods　The levels of plasma IL-33 and Da-jvGlu were detected in 126 patients of acute cerebral infarction and 10 normal controls.The correlation between IL-33 and cerebral infarction was analyzed.Results　Compared with those in normal control group and the pretherapy groups,the levels of IL-33 in large volume were significantly higher (P<0.05).After giving Shenqi glucose injection,the level of IL-33 in mid volume and small volume were significantly higher between groups (P<0.05).Compared with those in normal control group and the pretherapy groups,the level of Da-jvGlu in large volume was significantly higher (P<0.05).After giving Shenqi glucose injection,there was no significant difference of Da-jvGlu in statics between groups (P>0.05).Conclusion　Shenqi glucose injection can affect the levels of IL-33 to protect nerve cells and stabilize blood glucose concentration.

Osteoporosis （OP） and osteoarthritis （OA） are common bone diseases in clinic. OP is a systemic skeletal disease， and OA is a chronic degenerative joint disease with high prevalence and disability rates. With the advent of the aging population， the incidence rate of OA and OP is increasing year by year， and they have become common diseases of the elderly. The quality of life and physical and mental health of patients are severely affected by the above two bone diseases. Chinese medicine has a long history of treating bone diseases， with a good clinical effect on preventing and treating OP， OA， and other bone diseases with few side effects. It is one of the commonly used methods to treat bone diseases. Polysaccharides， as one of the active substances of Chinese medicine， have various pharmacological activities and a wide range of sources with low toxicity， and their effect cannot be ignored. The role of polysaccharides in the treatment of bone diseases has been deeply studied. It has been found that the mechanism of Chinese medicine polysaccharides in treating OP and OA involves multiple levels， targets， and pathways. Through the analysis and summary of the relevant literature on the mechanism of Chinese medicine polysaccharides in treating OP and OA， it was found that Chinese medicine polysaccharides mainly treated OP by regulating the bone dynamic balance between osteoblasts and osteoclasts and affecting bone marrow mesenchymal stem cells and bone microstructure. The mechanism of Chinese medicine polysaccharides in the treatment of OA is related to the regulation of chondrocyte growth， the increase in the proteoglycan and collagen content in the cartilage matrix， and the reduction of oxygen free radical content and inflammatory mediator level. This study aimed to further explore the internal relationship among mechanisms of Chinese medicine polysaccharides in the treatment of bone diseases， to provide relevant ideas for the study of Chinese medicine polysaccharides in the treatment of bone diseases.

Objective To assess the cleaning effect of central air conditioning ventilation systems. Methods During 2006—2007, the quality of 15 central air conditioning ventilation systems from 5 hotels in Tianjin were assessed before and after cleaning and disinfection. Results Before the central air conditioning ventilation systems being cleaned and disinfected, the medians of the dust in central air conditioning ventilation system, the aerobic bacterial counts in dust, the fungus counts in dust, the aerobic bacterial count in ventilation system, and the PM10 were 60.67 g/m2, 40 cfu/m2, 14 cfu/m2, 1 074 cfu/m3, 304 cfu/m3 and 0.09 mg/m3 respectively. After that, the medians were 0.26 g/m2, 0 cfu/m2, 0.2 cfu/m2, 219 cfu/m3, 121 cfu/m3 and 0.07 mg/m3 respectively. The eligible rates were 20%(3/15), 87.8%(36/41), 82.9%(34/41), 18.3%(11/60), 66.7%(40/60) and 65.7%(23/35) before the systems being cleaned and disinfected, by comparison, they were 100%(15/15), 100%(41/41), 100%(41/41), 91.7%(55/60), 96.7%(58/60) and 100%(35/35) after being cleaned and disinfected. Before the systems being cleaned and disinfected, the eligible rates of hemolytic streptococcus in ventilation systems, Legionella pneumophila in cooling water and condensed water were 100%(39/39), 80%(4/5) and 100%(5/5), after that, they were all 100%. Conclusion Cleaning and disinfecting is an effective way to improve the hygiene quality of central air conditioning ventilation systems. For protecting the people health, central air conditioning ventilation systems should be cleaned and disinfected regularly.

Objective:To investigate the protective effect of Wutou Chishizhi Wan on myocardial ischemia reperfusion injury （MIRI） in rats， and observe its effect on such mechanisms as coagulation function， vascular endothelial cells and oxidative stress in rats. Method:A total of 40 SD rats were randomly divided into normal group， model group， positive drug group （Urokinase group） and Wutou Chishizhi Wan group， with 10 rats in each group. Except for the normal group， rat myocardial ischemia-reperfusion injury models were established. The changes of heart rate （HR） at 10 min before ischemia， 30 min after ischemia and 30， 60， 120 min （T₀，T₁，T₂，T₃，T₄）， and the change of electrocardiogram （ECG） J point after modeling in rats were observed. The pathological changes of rat myocardial tissue were observed by hematoxylin-eosin （HE） staining. The changes of four indexes of coagulation ［prothrombin time （PT）， activated partial thromboplastin time （APTT）， thrombin time （TT）， fibrinogen content decreased significantly （FIB）］ in rats were observed. The contents of endothelin-1 （ET-1）， thromboxane A₂ （TXA₂） and prostacyclin （PGI₂） in serum and myocardium levels of superoxide dismutase （SOD）， malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） of MIRI rats were observed. Western blot assay was used for the detection of oxidative stress protein Keap1 and transcription factor-E2-related factor （Nrf2） expression levels in rat myocardial tissue. Result:Compared with the normal group， the ECG of MIRI rats showed significant myocardial ischemic injury-like changes， ST segment was significantly elevated， J point was significantly increased， and the incidences of HR in T₁， T₂， T₃ and T₄ were significantly reduced （P<0.05， P<0.01）. Compared with the model group， Wutou Chishizhi Wan significantly reduced ECG J-point changes in MIRI rats， while increased the incidence of HR in T₁， T₂， T₃ and T₄ （P<0.05， P<0.01）. Compared with the normal group， PT， APTT and TT in the model group were significantly shortened （P<0.01）， FIB content was significantly increased （P<0.01）， and the serum PGI₂ level decreased and TXA₂ and ET-1 levels increased significantly in the model group （P<0.01）. SOD content and GSH-Px activities of myocardial tissue in the model group were significantly reduced （P<0.01）， whereas the MDA content was increased （P<0.01）. Compared with the model group， PT of the Wutou Chishizhi Wan group was prolonged （P<0.05） and APTT slightly prolonged， TT significantly prolonged （P<0.01）， FIB content decreased （P<0.05）， serum PGI₂ increased （P<0.05）， TXA₂ and ET-1 decreased significantly in the Wutou Chishizhi Wan group （P<0.01）， myocardial MDA content decreased， and SOD content and GSP-Px activity increased significantly （P<0.01）. Meanwhile， the Wutou Chishizhi Wan group was able to activate the Keap1/Nrf2 signaling pathway， which significantly increased Nrf2 expression and significantly decreased Keap1 expression （P<0.01）. Conclusion:Wutou Chishizhi Wan group can protect myocardial injury in MIRI rats. The specific mechanism is to protect MIRI by regulating vascular endothelial cell homeostasis and oxidative stress levels and activating Keap1/Nrf2 signaling pathway.