To evaluate the diagnostic value of heterologous serum antibody for tuberculosis ,we detected the serum spec-imens from 102 patients with tuberculosis (included 73 pulmonary TB and 29 extrapulmonary TB) ,223 patients with other re-spiratory diseases and 100 healthy people were enrolled into the study .Results of clinical diagnosis as the golden standard were used to evaluate sensitivity and specificity of the tuberculosis antibody .Tuberculosis antibody (IgG/IgM ) ,sputum bacterial culture and sputum smear were used to test M .tuberculosis infection parallelly .Results were compared by chi-square test .Re-sults showed that the sensitivity and specificity of IgG/IgM tuberculosis antibodies in diagnosis of tuberculosis were respectively 74 .51% and 91 .64% .The sensitivity of IgG/IgM tuberculosis antibodies in diagnosis of pulmonary and extrapulmonary tuber-culosis were respectively 82 .19% and 55 .17% ,which were significant differences between the positive detection rate of pulmo-nary tuberculosis and extrapulmonary tuberculosis (P>0 .05) .The positive rate of IgG/IgM tuberculosis antibodies was higher than those in sputum culture and sputum smear (P＜0 .05) .A total of 102 cases of tuberculosis in patients in different age groups were analyzed ,and the positive detection rate of children and elderly group were 58 .33% and 36% ,respectively ,which were far below the rate in the youth group (96 .15% ) and the middle-aged group (89 .74% ) .The chi-square value for the differences in age group in the sensitivity of IgG/IgM tuberculosis antibodies was significant difference (P＜0 .05) .Six out of 425 cases of specimens were found as M .intracellulare ,and 2 as M .abscessus .However ,the IgG/IgM tuberculosis antibodies were negative in diagnosis of non-tuberculous mycobacteria .Antibody (IgG/IgM ) is sensitive ,rapid ,convenient ,and easy to manipulate the screening of tuberculosis .