No abstract available.
Cell Proliferation* ; Radiopharmaceuticals*

No abstract available.
Cell Proliferation* ; Cytomegalovirus* ; Humans*

No abstract available.
Alendronate ; Cell Proliferation

No abstract available.
Cell Proliferation* ; Female ; Humans ; Trophoblasts*

Two-stage carcinogenesis has been widely used as a method for evaluating precancerous lesion on animal experiments. Here we used diethylnitrosamine (DEN), a tumor trigger, and carbon tetrachloride (CCl4), a tumor promoter, to mimic the first two stages of hepatic carcinogenesis. Single intra-peritoneal injection of DEN 1% in combination with repeated intra-peritoneal injection of CCl4 4% for 16 weeks led to the formation of hyperplasia nodules on mouse livers. Microscopic examination also revealed dramatic changes in hepatocellular morphology and architecture: increased cell density and proliferation, abnormalities in cell division and nucleus structure. These changes were not observed in animal treated with NaCI or DEN alone. Repeated injection of CCl4 alone led to alterations on microscopic pictures but did not cause hyperplasia nodules. Our results indicate that DEN in combination of CCl4 causes abnormal proliferation of hepatic cells and evokes hepatic hyperplasia
Hepatocytes ; Cell Proliferation ; Carbon Tetrachloride

PURPOSE: The purpose of this study was to evaluate the influence of acid etching treatment on surface characteristics and biological response of glass-infiltrated zirconia. MATERIALS AND METHODS: A hundred zirconia specimens were divided into four groups depending on surface treatments: untreated zirconia (group Z); acid-etched zirconia (group ZE); glass-infiltrated zirconia (group ZG); and glass-infiltrated and acid-etched zirconia (group ZGE). Surface roughness, surface topography, surface morphology, and Vickers hardness of specimens were evaluated. For biological response test, MC3T3-E1 cell attachment and proliferation on surface of the specimens were examined. The data were statistically analyzed using one-way ANOVA and Tukey's HSD test at a significance level of 0.05. RESULTS: Group ZGE showed the highest surface roughness (Ra = 1.54 µm) compared with other groups (P < .05). Meanwhile, the hardness of group Z was significantly higher than those of other groups (P < .05). Cell attachment and cell proliferation were significantly higher in group ZGE (P < .05). CONCLUSION: We concluded that effective surface roughness on zirconia could be made by acid etching treatment after glass infiltration. This surface showed significantly enhanced osteoblast cell response.
Cell Proliferation ; Glass ; Hardness ; Osteoblasts

No abstract available.
Cell Proliferation* ; Nevus* ; Nevus, Pigmented*

Carcinogenic materials used in. occupational setting are thought to induce cancer by acting on DNA. BenzoCa)pyrene and the metabolites activated by the rat microsomal fraction were treated to the BALB/3T3 cells to see the formation of DNA-Protein Crosslinks (DPCs) and the repair. We measured the DPCs by the K-SDS assay according to Costa. The results are as follows: 1) The cytotoxicity results showed that viable cells were decreased by the increase of the dose of benzo[a]pyrene and microsomal activated metabolites and the metabolites treated cells showed more cytotoxicity. 2) The amounts of protein-crosslinked DNA in control cells are 690 ng/ml. The amounts were increased to 920 ng/ml in benzoCa)pyrene 0.1 microgram/ml treated cells, 720 ng/ml in benzo[a]pyrene 1 microgram/ml treated cells, 1,243 ng/ml in benzo[a]pyrene 10 microgram/ml, treated cells. The DPCs were measured higher in the metabolites treated cells than the benzo[a]pyrene treated cells 3) The DPCs were highest in the benzo[a]pyrene 10 microgram/ml + microsomal fraction treated cells among all treated cells. The DPCs were measured in those cells at 12 hour, 24 hour, 48 hour and 72 hour later to monitor the change of the amount of DPCs to see the repair of DPCs. The result showed that the amount of crosslinked DNA were decreased by the time. But considering the cell proliferation, the DPCs amount were not changed much and the repair did not seem to occur well.
Animals ; Cell Proliferation ; DNA ; Rats

No abstract available.
Cell Differentiation ; Cell Proliferation ; Fibronectins* ; Osteoblasts* ; Titanium

Cell Line, Tumor ; Cell Proliferation ; Glutaminase