1.Role of Cell Adhesion Molecule inAngiogenesis.
Journal of Korean Society of Endocrinology 2001;16(3):305-312
No abstract available.
Cell Adhesion*
2.A Potent Metastatic Factor in Squamous Cell Carcinoma: Laminin 332.
Soon Young KWON ; William B CARPENTER ; Philip M CARPENTER
Korean Journal of Otolaryngology - Head and Neck Surgery 2008;51(11):956-959
No abstract available.
Cell Adhesion Molecules
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Laminin
3.The Role of Cell Adhesion Molecules in the Modulation of Chondrocytes-extracellular Type II Collagen by Transforming Growth Factor-beta1.
Jin Woo LEE ; Eung Chick KANG ; Soo Bong HAHN ; Sung Jae KIM ; Yun Hee KIM ; Su Hyang KIM ; Sean P SCULLY
Journal of Korean Orthopaedic Research Society 2001;4(1):32-42
No Abstract Available.
Cell Adhesion Molecules*
;
Cell Adhesion*
;
Collagen Type II*
4.Integrin activation, focal adhesion maturation and tumor metastasis.
Meng-Wen HUANG ; Chang-Dong LIN ; Jian-Feng CHEN
Acta Physiologica Sinica 2021;73(2):151-159
Integrins are a large family of heterodimeric cell adhesion molecules composed of α and β subunits. Through interaction with their specific ligands, integrins mediate cell-cell and cell-extracellular matrix interactions. Via outside-in signaling, integrins can recruit cytoplasmic proteins to their intracellular domains and then cluster into supramolecular structures and trigger downstream signaling. Integrin activation is associated with a global conformation rearrangement from bent to extended in ectodomains and the separation of α and β subunit cytoplasmic domains. During cell migration, integrins regulate the focal adhesion dynamics and transmit forces between the extracellular matrix and the cell cytoskeleton. In tumor microenvironment, integrins on multiple kinds of cells could be activated, which modulates cell migration into tumor and contributes to angiogenesis and tumor metastasis. Here, we review the mechanism of integrin activation, dynamics of focal adhesions during cell migration and tumor metastasis.
Cell Adhesion
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Cell Adhesion Molecules
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Focal Adhesions
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Integrins
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Signal Transduction
5.Leukocyte-Endothelial Cell Adhesion Induced by Ischemia and Reperfusion Observed with in vivo Videomicroscopy.
Young Bae LEE ; Han Sug KANG ; Shin Byung PARK
Journal of Korean Neurosurgical Society 2000;29(10):1289-1295
No abstract available.
Cell Adhesion*
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Ischemia*
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Microscopy, Video*
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Reperfusion*
6.Biological Effects of Fibronectin Type III 10 domain on Human Osteoblast-like cells.
Chang Seok LEE ; Jun Hyeog JANG ; Tae Il KIM ; Yong Moo LEE ; In Chul RHYU ; Chong Pyoung CHUNG ; Soo Boo HAN ; Young KU
The Journal of the Korean Academy of Periodontology 2004;34(2):293-301
No abstract available.
Alkaline Phosphatase
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Cell Adhesion
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Fibronectins*
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Humans*
;
Osteoblasts
7.Effect of Quercetin on the Cell Cycle and Adhesion Molecules of NOD/SCID Mice with Acute B Lymphocytic Leukemia.
Li WANG ; Hong-Wei DAI ; Jun ZHENG ; Jiao ZHOU ; De-Sen CHEN
Journal of Experimental Hematology 2018;26(6):1616-1620
OBJECTIVE:
To investigate the effect of Quercetin on cell cycle and adhesive molecules of NOD.SCID mice with acule B lymphocytic leuaemia(B-ALL).
METHODS:
5×10 Nalm-6(B-ALL cell line) cells were injected into the tail vein of 48 NOD/SCID mice to establish the NOD/SCID mice with B-ALL. After 15 day, the NOD/SCID mice with B-ALL were randomly divided into 3 groups: salive group as control (injection with saline of 0.2 ml/mouse), cyclophos-phamid group (injection with cyclophosphamide of 100µg/kg) and quercetin group(injection with quercetin of 3 mg/kg). After treatment for 21 d, the perecntage of Nalm-6 cells in G1, G2, M and S phases was detected by flow cytonetry; the B lymphocytes Nalm-6 cells, neutrophils and WBC in while blood were counted before and after treatment; the expression of intercellalar. Adhesion molecole-1(FCAIU-1), vascular cell adhesion molecule-1(VCAM-1) and P-selectin was detected by double autibody soundwich method.
RESULTS:
Compared with level before treatment, the expression of ICAM-1, VCAM-1 and P-selectin decreased after treatment with guercetin, The hemogram showed that the peripheral blood nentrophil level obviously increased, while the levels of B lymphocytes, Nalm-6 cells and WBC count decreased obviously after treatment with guercetin. The cell proliferatim rario in G0/G1 phase decreased, yet the cell proliferation ratio in S and G2/M phases increased after treatment with guercetin.
CONCLUSION
The guercetin can decrease the intercellular adhesion through inhibition of ICAM-1 expression, and arrests Nalm-6 cells in S and G2/M phases. The guercetin has obviously inhibitory effect on B-ALL cells.
Animals
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Cell Adhesion
;
Cell Adhesion Molecules
;
Intercellular Adhesion Molecule-1
;
Leukemia, B-Cell
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Mice
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Mice, Inbred NOD
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Mice, SCID
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Quercetin
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Vascular Cell Adhesion Molecule-1
8.Effects of RGD peptides-grafted porous tantalum on morphological change of MG63 osteoblasts-tantalum conjunctive interface and expression of osteogenesis factors.
Hong Quan GAN ; Qian WANG ; Hui ZHANG ; Xin LIU ; Hua Min DENG ; Hui Ping SONG ; Zhi Qiang WANG ; Qi Jia LI
Journal of Peking University(Health Sciences) 2018;50(1):176-182
OBJECTIVE:
To investigate the effects of the Arg-Gly-Asp polypeptedes (RGD) peptides-modified porous tantalum surface on osteoblasts morphology and expressions of osteogenesis factors, and to evaluate RGD peptides promotes junctura ossium of tantalum-bone interface in vivo.
METHODS:
RGD peptides of different concentrations (1 g/L, 5 g/L, and 10 g/L) were loaded to porous tantalum slices with a diameter of 10 mm and a thickness of 3 mm by physical absorption. The 3rd generation of MG63 cells were co-cultured with tantalum and divided into 4 groups: Ta-cells (control) group, 1 g/L cells/Ta/RGD group, 5 g/L cells/Ta/RGD group, and 10 g/L cells/Ta/RGD group. Porous tantalum compo-sites and osteoblasts-tantalum interface were observed by scanning electron microscopy. The adhesion rate of osteoblasts was detected and immunocytochemistry was used to detect the expressions of filamentous actin (F-actin), osteocalcin (OC) and fibronectin (FN).
RESULTS:
The scanning electron microscope (SEM) revealed that osteoblasts distributed on the surface of porous tantalum and secreted extracellular matrix on outside and inner of micro-pores. The osteoblasts adhesion rate on porous tantalum modified with RGD was higher than that in the unmodified porous tantalum at the end of 24, 48, and 72 hours. The best adhesion effect was got in 5 g/L cells/Ta/RGD group at hour 48 [(68.07±3.80) vs. (23.40±4.39), P<0.05]. The results of immunocytochemistry showed that the expressions intensity of F-actin, OC and FN in osteoblasts on porous tantalum modified groups with RGD were stronger than that in the unmodified groups, and the expressions of 5 g/L cells/Ta/RGD group were significantly higher than those in the 10 g/L group and 1 g/L group [OC: (18.08±0.08) vs. (15.14±0.19), P<0.05; (18.08±0.08) vs. (14.04±0.61), P<0.05. FN: (24.60±0.98) vs. (15.90±0.53), P<0.05; (24.60±0.98) vs. (15.30±0.42), P<0.05. F-actin: (29.20±1.31) vs. (24.50±1.51), P<0.05; (29.20±1.31) vs. (16.92±0.40), P<0.05]. Correspondingly F-actin in osteoblasts was showed in longitudinal arrangement, and the expressions intensity was stronger than those OC and FN.
CONCLUSION
The RGD peptides is beneficial to enhance adhesion of osteoblast, spreading and reorganization of cytoskeleton on porous tantalum surface and improve the interface morphology, further promoting osteoblasts-tantalum conjunctive interface osseointegration.
Cell Adhesion
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Oligopeptides
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Osteoblasts/physiology*
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Osteogenesis
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Tantalum
9.Effects of SLA surface treated with NaOH on surface characteristics and response of osteoblast-like cell.
Jin Chul PARK ; Joo Hyeun KIM ; Eun Sook KANG ; Jae Jun RYU ; Jung Bo HUH
The Journal of Korean Academy of Prosthodontics 2014;52(3):211-221
PURPOSE: The purpose of this study was to evaluate the surface characteristics and response of osteoblast-like cell at SLA surface treated with NaOH. MATERIALS AND METHODS: Three kinds of specimens were fabricated for the experiment groups. Control group was a machined surface, SLA group was a conventionally SLA treated surface, and SLA/NaOH gorup was SLA surface treated with NaOH. To evaluate the surface characteristics, the surface elemental composition (XPS), surface roughness and surface contact angle were evaluated in each group. And the cytotoxicity, cell adhesion, cell proliferation and ATP activity of osteoblast-like cells (MG-63 cells) were compared in each group for evaluatation of the cell responses. Statistical comparisons between groups were carried out via one-way ANOVA using the SPSS software (SPSS Inc., Chicago, USA), and then performed multiple comparisons. The differences were considered statistically significant at P<.05. RESULTS: SLA surface treated with NaOH (SLA / NaOH group) was changed to hydrophilic surface. All groups did not show the cytotoxicity to the MG-63. In cell adhesion studies, SLA / NaOH group showed the higher degree of adhesion than anothers (P<.05), Up to 7 days of incubation, the proliferation was showed the increasing tendency in all groups but SLA / NaOH group showed the highest cell proliferation between the three groups (P<.05). At 7 days of incubation, there was no difference in ALP activities between the three groups, but at 14 days, SLA / NaOH group showed significant increase in ALP activities (P<.05). CONCLUSION: In this study, SLA surface treated with NaOH promoted cell adhesion, proliferation and differentiation. It means that SLA/NaOH group is possible to promote osseointegration of implants.
Adenosine Triphosphate
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Cell Adhesion
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Cell Culture Techniques
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Cell Proliferation
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Osseointegration
10.Enhanced Expression of Cell Adhesion Molecules in the Aorta of Diabetic Mice is Mediated by gp91phox-derived Superoxide.
Mi Ran YUN ; Jong Jae KIM ; Sun Mi LEE ; Hye Jin HEO ; Sun Sik BAE ; Chi Dae KIM
The Korean Journal of Physiology and Pharmacology 2005;9(2):109-115
Endothelial activation and subsequent recruitment of inflammatory cells are important steps in atherogenesis. The increased levels of cell adhesion molecules (CAM) have been identified in diabetic vasculatures, but the underlying mechanisms remain unclear. To determine the relationship among vascular production of superoxide, expression of CAM and diabetes, superoxide generation and expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E- and P-selectin in the aorta from control (C57BL/6J) and diabetic mice (ob/ob) were measured. In situ staining for superoxide using dihydroethidium showed an increased superoxide production in diabetic aorta, accompanied with an enhanced NAD (P) H oxidase activity. Immunohistochemical analysis revealed that the endothelial expression of ICAM-1 (3.5+/-0.4) and VCAM-1 (3.8+/-0.3) in diabetic aorta was significantly higher than those in control aorta (0.9+/-0.5 and 1.6+/-0.3, respectively), accompanied with the enhanced expression of gp91phox, a membrane subunit of NAD (P) H oixdase. Furthermore, there was a strong positive correlation (r=0.89, P< 0.01 in ICAM-1 and r=0.88, P< 0.01 in VCAM-1) between ICAM-1/VCAM-1 expression and vascular production of superoxide. The present data indicate that the increased production of superoxide via NAD (P) H oxidase may explain the enhanced expression of CAM in diabetic vasculatures.
Animals
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Aorta*
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Atherosclerosis
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Cell Adhesion Molecules*
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Cell Adhesion*
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E-Selectin
;
Intercellular Adhesion Molecule-1
;
Membranes
;
Mice*
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NAD
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Oxidoreductases
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P-Selectin
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Superoxides*
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Vascular Cell Adhesion Molecule-1