1.Application of rapid sequence induce nasotracheal intubation with fiberoptic bronchoscope in patients with cervical vertebra injury
Cehua OU ; Xian JIANG ; Changhe REN ; Xiaobin WANG
Chinese Journal of Postgraduates of Medicine 2008;31(30):25-27
Objective To compare the appfication of rapid sequence induce nasotracheal intobation with awake nasotracheal intubation with fiberoptic bronchoscope (FOB) in patients with cervical vertebra injury.Methods Forty patients with cervical vertebra injury were randomly divided into group (rapid sequence induce nasotracheal intubation with FOB) and group II (awake nasotracheal intubation with FOB),20 patients in each group.Bp,HR,SpO2 before and during intubation,intubafion time and cases of re-intubation were observed.Results SpO2 maintained normal during intubation.Between group I and group II,there was no significance in intubation lime [(3.12±0.52)min and (3.34±0.65)mini and cases of re-intubafion (2 cases and 1 case) (P > 0.05 ).During intubation,MAP and HR inceased significantly in group II than those in group I (P < 0.05 ).Conclusion The application of rapid sequence induce nasotracheal intubation with FOB is better than awake nasotracheal intubation with FOB in patients with cervical vertebra injury,it is safe and valid.
2.Efficacy of cold preservation solution containing desferrioxamine in protecting rat hearts
Jiali WU ; Song SU ; Xuping HU ; Cehua OU
Chinese Journal of Anesthesiology 2016;36(3):325-327
Objective To evaluate the efficacy of cold preservation solution containing desferrioxamine (DFO) in protecting the rat hearts.Methods Pathogen-free male Sprague-Dawley rats,weighing 300-350 g,were used in the study.Thirty-two isolated rat hearts were equally and randomly divided into control group (C group) and DFO group using a random number table.Hearts of rats were stored for 6 h in 4 ℃ histidine-tryptophan-ketoglutarate (HTK) solution in group C.DFO was added to HTK solution (DFO concentration 100 μmol/L),and hearts of rats were stored for 6 h in 4 ℃ HTK solution in group DFO.At 6 h of cold storage,creatine kinase and lactate dehydrogenase activities in the cold preservation solution were determined.Myocardial specimens were obtained from the apex,cut into sections which were stained with haematoxylin and eosin,and examined under a microscope.The pathological changes of myocardial tissues were scored.The content of malondialdehydc in myocardial tissues was determined using thiobarbitnric acid method,and hypoxia-inducible factor-1α protein and mRNA expression in myocardial tissues was detected by real-time reverse transcriptase polymerase chain reaction and Western blot.Results Compared with group C,the creatine kinase and lactate dehydrogenase activities in the cold preservation solution,malondialdehyde content in myocardial tissues,and pathological scores were significantly decreased,and the expression of hypoxia-inducible factor-1α protein and mRNA expression in myocardial tissues was significantly up-regulated in group DFO (P< 0.05).Conclusion Cold preservation solution containing DFO can protect the rat hearts effectively,and the mechanism is related to up-regulation of the expression of hypoxia-inducible factor-1α.
3.Acute stress affects the PRA and AngⅡ levels in plasma and the cytoskeletons in hippocampus in adult rats
Guogang SUN ; Cehua OU ; Bo CHEN ; Yue CHEN ; Guangbi FAN
Chinese Journal of Pathophysiology 2000;0(10):-
AIM:To observe the changes in plasma rennin activity(PRA)and angiotensin Ⅱ(AngⅡ)level and the cytoskeletons in dorsal hippocampus(DH)in male and female stressed rats.METHODS:The adult Sprague-Dawley male and femal rats were stressed for 6 h per day.Three days later,the levels of PRA and AngⅡin plasma were determined by radioimmunoassay,and the expression of nestin and NF200 in dentate gyrus(DG)and CA3 regions were observed with immunohistochemical staining.RESULTS:①The levels of PRA in plasma of male or female rats were decreased in stressed rats compared with control groups(P
4.Intravenous injection of 20% lipid emulsion reverses neurotransmitters imbalance in central nerve system induced by local anesthetics
Li LIU ; Yue ZHOU ; Liqun MO ; Cehua OU ; Henglin SHI ; Gangming WU
The Journal of Clinical Anesthesiology 2016;32(3):273-276
Objective To investigate the effect of intravenous injection of 20% lipid emulsion on neurotransmitters imbalance in central nerve system induced by local anesthetics.Methods Thirty adult Sprague-Dawley rats were randomly divided into two groups:normal group (group C,n =10) and local anesthetics group (group L,n =20).Based on different treatment,the rats in group L were assigned into two subgroup:control group (group S,n =10)and 20% long-chain lipid emulsion in-jection group (group I,n =10).The rats of group L were injected with 0.75% levobupivacaine at 50μl/min from the right ventricle of brain.When convulsions wave of electroencephalogram EEG ap-peared,20% long-chain lipid emulsion was administrated continuously 0.25 ml·kg-1 ·min-1 (group I)or normal saline (group S).The rats in group C were without any treatment.All the rats were con-tinously monitored by EEG,ECG and SpO 2 .The primary outcome included seizure duration and threshold and rats’mortality of postoperative 24 h.Secondary outcome included the concentrations of neurotransmitters GABA and Glu in cerebrospinal fluid,expression of NMDAR1 in cortex,Glu/GA-BA concentration ratio (Glu/GABA),and analysis the correlation between expression of Glu and NMDAR1.Results The convulsion time and mortality of rats in group S were significantly increased compared with those in group I (P <0.05).The seizure threshold of levobupivacaine was much lower in group S than group I (P <0.05).Compared with group C,the concentrations of Glu and GABA in cerebrospinal fluid,expression of NMDAR1 in hippocampus and Glu/GABA significantly increased in groups S and I(P < 0.05 ),and at same time these data in group S were significantly higher than group I (P < 0.05 ).There is a positive correlation between concentration of Glu in CSF and expression of NMDAR1 in hippocampus (r =0.884,P <0.01 ).Conclusion Intravenous infusion of 20% long-chain lipid emulsion can effectively improve neurotransmitters imbalance in central nerve system induced by intraventricular injection of local anesthetics.
5.Study on dexmedetomidine combined with remote ischemic postconditioning for enhancing cerebral protective effect
Gang LI ; Yuan WANG ; Jiaomei ZHAO ; Gangming WU ; Cehua OU ; Jicheng WEI
Chongqing Medicine 2014;(36):4849-4851
Objective To evaluate the the protectice effect of dexmedetomidine combined limb remote ischemic postcondition on alleviating focal cerebral ischemic reperfusion injury in rats .Methods 48 healthy adult male SD rats were randomly divided into 4 groups(n= 12) :control group(C) ,limb remote ischemic postcondition group(R) and dexmedetomidine postconditioning group(D) and combination group(R/D) .The rat model of focal cerebral ischemic reperfusion injury was induced by middle cerebral artery oc‐clusion(MCAO) .The group C only received MCAO ,the left femoral artery was isolated without blocking ;the group R received 120 min brain ischemia ,the left femoral artery was occluded by 3 cycles of 10 min occlusion/10 min reperfusion before brain reperfu‐sion ;the group D received dexmedetomidine 3 μg/kg by intraperitoneal injection before brain reperfusion .The group R/D combined the above two kinds of processing method .The neurologic function was evaluated at 24 h of reperfusion and then the rats were sac‐rificed at 48 h of reperfusion .The brain was removed for determining the cerebral infarct volume .Results The neurologic function scores after 24 h reperfusion in the group D ,R and R/D were superior to those in the group C (P< 0 .01) .The rat cerebral infarct volume percentages after 48 h reperfusion in the group D ,R and R/D were significantly lower than those in the group C ( P <0 .01) .The infarct area volume percentage in the group R/D was significantly lower than that in the group R ,the difference showed statitistical significance(P< 0 .01) .The infarct volume percentage in group R/D was significantly decreased compared with the group D(P< 0 .05) .Conclusion Both dexmedetomidine and limb remote ischemic postcondition can attenuate the focal cerebral is‐chemic reperfusion injury in rats .Their combination can significantly reduce the cerebral infarction volume and has synergic protec‐tion effect .
6.Effects of hydrogen gas inhalation on cerebral oxidative stress and inflammation after intestinal ischemia/reperfusion in rats
Ye CHEN ; Guoqing HHUANG ; Shuzhi ZHOU ; Jun ZHOU ; Gangming WU ; Cehua OU ; Xiaobin WANG
Chinese Journal of Organ Transplantation 2014;35(4):237-242
Objective To investigate the effects of hydrogen gas inhalation on cerebral oxidative stress and inflammation after intestinal ischemia/reperfusion (I/R) in rats and to understand the mechanism of(I/R neuroprotection.Method Forty-eight healthy male SD rats weighing 285-350 g were randomly allocated to one of 3 groups (n =16 each group):sham operation group (Sham),intestinal I/R group (I/R) and intestinal IR plus hydrogen gas inhalation group (IR + H2).The I/R model was produced by occlusion of superior mesenteric artery (SMA) for 90 min followed by reperfusion.Inhalation of 2% hydrogen gas was performed immediately after I/R for 3 h.All animals were sacrificed at 24 h after reperfusion in each group.Brain tissues of 8 animals in each group were harvested for detection of microglia by immunohistochemistry.The remaining 8 rats in each group were used for the following indicators analysis.The protein level of ionized calcium-binding adaptor molecular 1 (Iba-1,a marker of microglia) in the cortex was detected by Western blotting.The concentrations of ROS,MDA,IL-1β,IL-6,TNF-α,T-NOS,iNOS and NO in the cortex were measured.The MPO content and SOD activity were also measured.Result The Iba-1 staining was light in Sham group.However,the expression of Iba-1 was increased in I/R group,and H2 inhibited the expression of Iba-l.As compared with Sham group,the Iba-1 protein expression and the number of Iba-1 positive cells were increased significantly in I/R and I/R+ H2groups (P<0.01 or 0.05).As compared with Sham group,ROS,MDA,IL-1β,IL-6,TNF-α,T-NOS,iNOS and NO levels,and MPO activity were also increased in I/R and I/R + H2groups (P<0.01 or 0.05).As compared with I/R group,the above indicators in I/R + H2 group were markedly improved (P<0.05 or 0.01).Conclusion The inhalation H2 could inhibit intestinal I/R-induced activation of microglia and reduce cerebral oxidative stress and inflarnmatory response in rats.
7.Efficacy and safety of botulinum toxin type A combined with pulsed radiofrequency in the treatment of postherpetic neuralgia
Lei HE ; Yue ZHANG ; Cehua OU ; Fubo LI
The Journal of Practical Medicine 2024;40(6):833-837
Objective Exploring the efficacy and safety of botulinum toxin type A(BTX-A)combined with pulsed radiofrequency(PRF)in the treatment of postherpetic neuralgia(PHN).Methods A total of 80 patients with PHN were collected.They were randomly divided into experimental group(Group B)and control group(Group C),Group B was treated with BTX-A intradermal injection combined with PRF,and Group C was treated with lidocaine intradermal injection combined with PRF.Numeric pain score(NRS),Simplified McGill Pain Questionnaire(SF-MPQ)and Sleep Quality Score(QS)were used to assess the patients'pain level and sleep quality preoperatively,1,3,and 7 days postoperatively,and 1,2,and 3 months postoperatively.The patients'postoperative adverse reac-tions were collected.Interleukin-1β(IL-1β)and calcitonin gene-related peptide(CGRP)levels in patients'serum were measured preoperatively and 3 days postoperatively.Results The NRS scores,SF-MPQ scores,and QS scores of group B and group C were significantly lower at all postoperative time points compared to preoperative ones(P<0.05).The NRS and SF-MPQ score were significantly lower in group B at 1,2,and 3 months postoperatively compared with group C(P<0.05);and group B had significantly lower QS scores at 2 and 3 months postoperatively(P<0.05).The effective rate of pain relief at 3 months postoperatively in group B(90%)was statistically signifi-cant(P<0.05)compared with group C(56.7%).No serious adverse reactions occurred in either group.The levels of IL-1β and CGRP in serum of patients in both groups were significantly decreased at 3 days after surgery compared with the preoperative period,and the degree of decrease of IL-1β and CGRP in group B was more significant than that in group C(P<0.05).Conclusion BTX-A combined with PRF treatment for PHN can effectively reduce its pain level,improve the quality of sleep,and is safe.