1.Irradiation Time of Photodynamic Therapy to the Number of Lactobacillus acidophilus
Sri Kunarti ; Nunik Nuraini ; Farida Widhi Astuti ; Cecilia GJ Lunardi ; Lulytha Rahmanike Putri
Archives of Orofacial Sciences 2021;16(SUPP 1):33-36
ABSTRACT
Lactobacillus acidophilus (L. acidophilus) is one of the etiological agents for dental caries and dominant in
the deep carious lesion. L. acidophilus has also been identified in persistent root canal infection and also
related to the failure of endodontic treatment. Photodynamic therapy is a therapeutic process involving
the combination of a nontoxic photosensitizer and a light source. The excited photosensitizer reacts with
reactive oxygen species (ROS), which induce injury and death of the microorganism. This study aimed
to prove the effect of irradiation time of photodynamic therapy to the number of L. acidophilus. Forty-two Eppendorf tubes were treated with 0.5 ml L. acidophilus distributed into seven groups. Group 1 as
the control group received no treatment. Groups 2, 3, 4, 5, 6 and 7 were treated with a combination of
0.5 ml toluidine blue O (TBO) as a photosensitizer and 630 nm photoactivated (Fotosan®) exposure
time for 10, 20, 30, 40, 50 and 60 sec. Then, all were stored in an incubator of 37ºC for 48 h. Later,
the colony-forming unit (CFU) was counted for each group. There were significant differences in the
number of L. acidophilus in CFU of the various irradiation times. The longer the photodynamic therapy
irradiation was, the lesser the number of live L. acidophilus became. At 50 sec and 60 sec irradiation,
none of the L. acidophilus was found alive.
Photochemotherapy
;
Lactobacillus acidophilus