Kawasakd disease(KD) or incomplete KD(IKD) is a systemic vasculitis predominantly affecting young children.Clinical manifestations include fever with no obvious origin,skin damage,abnormalities of mucous membrane and lymph nodes.The disease is often accompanied by serious cardiovascular complications such as coronary artery aneurysm,therefore it attracts more and more attention.But arthritis or arthralgia complicates over one-third of KD or IKD patients.So early-onset arthritis is often indistinguishable from early juvenile idiopathic arthritis,especially systemic-onset juvenile idiopathic arthritis(SoJIA).KD and SoJIA are considered to be different diagnoses for children with long-term fever,rash,swollen lymph nodes,and more importantly,the treatment procedures are very different,but it is difficult to completely distinguish the two diseases,especially the IKD and early SoJIA.So in this paper,we will summarize the relationship between KD and arthritis in children,which aims to remind clinicians to pay attention for the diagnosis of KD and IKD.We suggest that physicians should be alert of the fact that they must individualize every patient's management,rather than merely care by the guidelines,which will delay the treatment.

FPGA is a kind of powerful programmable chip used in the development of digital system, and it has such advantages as large capacity, reliability and easy modification. The design of FPGA system is mainly through software method which is easy to grasp. Compared with legacy discrete circuit or ASIC, more and more FPGAs are adopted in digital systems for low cost and fast speed. On the other hand, digital system plays an important role in control system of medical equipments, so FPGA is expected to be widely used in almost all fields of medical equipment.

AIM: To investigate the concentration of bufadienolides in Liushen Pill from two enterprises. METHODS: Four kinds of bufadienolides were simultaneously determined by using HPLC with DAD detector. RESULTS: Recoveries obtained from the four kinds of bufadienolides were higher than 96%,the linearity was in the range of 1 ?g/mL-100 ?g/mL.The concentration of bufadienolides in Liushen Pill was significantly higher than that in other related TCM compatibilities. CONCLUSION: The well-distributed compatibility in Liushen Pill has an influence on the solubilization of bufadienolides,may be in relation to eahancing to release.

Objective: To investigate a possible role of extracellular signal-regulated kinase (ERK) 1/2/ calcium-activated neutral protease (CANP) signaling pathway in 17β-estradiol (E2)-induced proliferation of human breast cancer cell line MCF-7. Methods: MCF-7 cells were pretreated with ERK1/2 inhibitor PD98059 or CANP inhibitor calpeptin, and then treated with 1×10-8 mol/L E2. MTT assay was employed to determine the effect on cell proliferation. Cell cycle distribution was detected by flow cytometry (FCM). The expression levels of phosphorylated-ERK (p-ERK), total ERK and CANP small subunit Capn4 proteins were examined by Western blotting. Results: The proliferation rate was increased in MCF-7 cells treated with 1×10-8 mol/L E2 for 24 h (P<0.01). The number of cells in G0/ G1 phase was decreased, and the accelerated G1/S transition was observed. Calpeptin could significantly inhibited E2-induced proliferation of MCF-7 cells (P<0.01). The number of cells in G0/G1 phase was increased, and the delayed G1/S transition was observed. The expression of p-ERK was rapidly up-regulated (during 10 minutes) induced by E2 (P<0.01), and this effect persisted for at least 16 h; but there was no obvious change in the expression of total ERK. E2-induced ERK phosphorylation in MCF-7 cells was significantly inhibited by PD98059 (P<0.01). The expression of Capn4 protein was rapidly up-regulated in E2-induced MCF-7 cells ( P<0.01) while significantly inhibited by PD98059 or calpeptin (P<0.01) in MCF-7 cells. Conclusion: The rapid signaling pathway of ERK/ CANP is involved in E2-induced proliferation of human breast cancer cells.

Objective: To investigate the effect of silencing Smad4 gene expression by using small interfering RNA (siRNA) on proliferation and migration of ECV304 cell line and explore the role of Smad4 in angiogenesis. Methods: Two siRNA sequences targeting Smad4 gene including siRNA1 and siRNA2 were designed and then synthetized. The two sequences of siRNA were transfected into ECV304 cells via lipofectamine mediation. The alteration of Smad4 mRNA and protein expression was examined by real-time RT-PCR and Western blot, respectively. The proliferation capacity of ECV304 cells was measured by cell cycle analysis and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) flow cytometry. The monostratal wound healing test was used to determine the migration capacity of ECV304 cells. Results: siRNA2 was successfully screened out of the two sequences. Transfection of siRNA2 significantly knocked down the Smad4 mRNA and protein expressions. The proliferation and migration capacity of ECV304 cells are significantly enhanced after transfection with siRNA2. Conclusions: Highly effective and specific siRNA targeting Smad4 gene can be successfully screened by RNA interference technique. Selective silencing of Smad 4 gene greatly promotes the proliferation and migration of ECV304 cells.

Dystonia is common in children with cerebral palsy. It can cause motor dysfunction, and is frequently associated with altered speech articulation, abnormal swallowing, and excessive drooling. Although trihexyphenidyl (Artane) is an anticholinergic agent with a long history of use in the management of dystonia in adults, information is limited regarding the use of trihexyphenidyl in children. This study reviewed the clinical experience of the use of trihexyphenidyl in children with cerebral palsy for dystonia, with special emphasis on benefits and tolerability.

Translumbar amputation, also known as hemicorporectomy, is a life-saving procedure for patients with a life-threatening diagnosis but with a normal life expectancy. In the surgical procedure, the pelvis, pelvic contents, lower extremities and external genitalia are removed following transection of the lumbar spine. The operation makes the patient lose the ability of being upright. With the interdisciplinary cooperation of doctors, nurses, prosthetists, psychologists, vocational rehabilitation workers and social rehabilitation workers, the patient can realize most of independent activities of daily living through rehabilitation. The rehabilitation associated with the procedure is reviewed.

Objective To study the clinical effect of treating mild and moderate hypertension in the elderly with flavored liuwei dihuang decoction.Methods From January 2016 to January 2017,120 cases of elderly patients of mild and moderate hypertension with the diagnosis and treatment in our hospital are divided into two groups, the control group was treated with nifedipine zyban, and the observation group was treated with flavored liuwei dihuang decoction;Record two groups of patients with various experiment data, make an analysis of the relevant data obtained and discuss the clinical effect of flavored liuwei dihuang decoction to treat the mild and moderate elderly hypertension.Results The clinical results of the treatment of observation group were better than that of the control group.The patients had fewer adverse reactions, and the differences between two groups were statistically significant (P< 0.05).Conclusion The treatment which using the flavored liuwei dihuang decoction in the mild and moderate elderly hypertension receives significant improvement in the clinical symptoms and little adverse reactions.It is worth using widely in clinical practice.

Objective To investigate the changes of platelet activating factor ( PAF) in serum from patients with hypertensive disorder complicating pregnancy .Methods The specimens from 138 women were obtained at Department of Obstetrics , Xinhua Hospital , School of Medicine , Shanghai Jiaotong Uni-versity, between June 2008 and March 2010.They were divided into two groups:women( n =63) with hy-pertensive disorder complicating pregnancy and normal pregnant women ( n =75 ) ( control group ) .All of them delivered until maturity .ELISA was used to detect the levels of PAF in those women's serum and in the umbilical cord serum .Results The levels of PAF in hypertensive disorder complicating pregnancy group (27.46 ±13.37 ) ng/ml were no significantly different from the control group (25.48 ±11.05 ) ng/ml( P >0.05 ) .With the hypertensive disorder complicating pregnancy more deteriorative , the levels of women ser-um PAF would be higher .The levels of women's serum PAF in hypertensive disorder complicating pregnancy group were significantly higher than the umbilical cord serum [0.00(0.00-8.83) ng/mL]( P <0.05). The levels of women's serum PAF in control group were also significantly higher than the umbilical cord ser -um[0.00(0.00-7.80)ng/mL]( P <0.05).Conclusion There are some relationships between PAF and hypertensive disorder complicating pregnancy .

Quantum dots (QD),a kind of nanocrystal,are made from Ⅱ-Ⅵ or Ⅲ-Ⅴ group elements.It has been reported that compared with current conventional fluorescent markers,QD have excellent optical properties such as strong fluorescence,photochemical stability and can be used for simultaneous multi-channel imaging.Meanwhile,as nanoparticle,QD can be easily surface modified with a variety of biological molecular and can reach cancer cells easily by penetrating the tumor angiogenesis.Therefore,QD have unique advantages in targeted real-time visual imaging of in vivo tumor and have great prospects in the individual diagnosis and treatment of tumor.However,the long-term biosafety after QD into the body still needs further study.