OBJECTIVETo provide suggestion for MDAEs monitoring with analyzing the features of the 1028 reports of MDAEs.

METHODSWith retrospective case study, this paper analyzed 1 028 MDAE reports in Guiyang during 2012.

RESULTSThe intrauterine device (IUD) caused the higher proportion of adverse events with serious degree. Monitoring consciousness of grassroots users needs to be improved. The quality of the reports should be enhanced.

CONCLUSIONSMore attention should be paid to monitoring on IUD. The level of MDAEs monitoring should be improved through carrying out targeted training and publicity, establishing and improving MDAEs monitoring network.

Vaccine is expected to be a promising tool for malaria prevention and control.However, safe and effe-ctive malaria vaccine is not yet available for field use so far.They can be pre-erythrocytic stage vaccine, blood stage vac-cine and transmission-blocking vaccine.This review summarizes the progress of the vaccine development in the recent field trials.

Objective:To summarize the differences and similarities in the detection methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition so as to provide reference for the improve-ment of the detection methods for residual solvents in Safety and Technical Standards for Cosmetics. Methods:The type and limitations of residual solvents and the characteristics of the test methods for residual solvents between Safety and Technical Standards for Cosmetics 2015 edition and Chinese Pharmacopoeia 2015 edition were compared and analyzed. Results: The detection methods for residual sol-vents in Chinese Pharmacopoeia were more detailed. The detection methods for residual solvents in Safety and Technical Standards for Cosmetics were general detection method, and the process could be applied in the detection of more solvents. Some detection methods were short of limitations. Conclusion:The control of residual solvents in cosmetics should be improved if the limitations table of the limiting used solvents is introduced into Safety and Technical Standards for Cosmetics referring to Chinese Pharmacopoeia and increase the types of residual solvents detected by the general methods.

AIM: To determine the effect of agmatine on the proliferation of pulmonary artery smooth muscle cells (PASMCs) induced by hypoxia. METHODS: Primary culture of rat PASMCs was prepared from adult male Wistar rat pulmonary artery by the method of tissue block anchorage. PASMCs were divided into two groups: control group and agmatine-treated group. The activity of LDH in the medium was measured by chromatometry. [3H]-TdR incorporation was measured by liquid scintillation counting. The cell cycle was measured by flow cytometry, and the PCNA content was measured by image analysis. RESULTS: Agmatine did not exert significant effect on the activity of LDH in the medium, but significantly decreased the [3H]-TdR incorporation and PCNA content of PASMCs. Agmatine significantly decreased the cell ratio of G_2/M phase and increased the cell ratio of G_0/G_1 phase. With the increment of the concentration of agmatine, [3H]-TdR incorporation was significantly decreased. CONCLUSION: Agmatine has no significant cytotoxic effect on PASMCs and agmatine dose-dependently inhibits the proliferation of rat PASMCs induced by hypoxia.

3D printing technology has become the focus of the study on rapid proyotyping technology in recent years. This paper introduced the principle, material and equipments of 3D printing, as well as the foot orthosis. This paper also summarized the application of 3D printing in custom-made foot orthosis, and the differences between 3D printing and traditional technology from the aspect of structure design, material, and clinical application, etc., and discussed the problems and future development of 3D printing.

Objective:To prove the pharmacological action of Qingfei Capsules.Methods: Some tests such as suppressing cough, dispelling phlegm, calming panting, anti-inflammation, anti-virus, its effects on the nonspecific immunity and cellular immunity of mice were carried out. Results: Qingfei Capsules could prolong the incubation period of cough, wheezing and panting, possess the actions of dispelling phlegm and inhibiting inflammation, raise the nonspecific immunity of body, promote humoral immunity, and raise the cellular immune function.Conclusions: These results provide a basis for clinical application of Qingfei Capsules.

Objective　To develop a new method to induce the gene transfection in high efficiency for eukaryotic cells in vitro. Methods　 Four kinds of p14ARF gene primarily deleted human carcinoma cell line including H460,A549,U251,and PC-3 were transfected with the human p14ARF expression vector (pCI-neo-p14ARF) by using the new nonliposomal transfection reagent Fugene 6. The efficiency of gene transfer was determined by screening the cells in G418. Results　 After 21 days' selection, G418-resistant clones were shown in all the transfected plate. PCR product of p14ARF gene was positive in all the G418-resistant clones. Cytotoxicity of Fugene 6 was detected. The cell proliferation activity was not affected when it was cultured in a high dose of Fugene 6. Conclusion　 These results demonstrate that Fugene 6 is a rapid, feasible, reproducible, and noncytotoxic gene transfection approach for eukaryotic expression vector in vitro.

The relative factors influencing the level of psychosomatic health and driving safety were investigated in 203 army drivers. The results indicated that psychosomatic disorders, especially more prominent in subscales of the eye and ear, respiratory system, skeleton and muscle, and depression might occur. The main factors influencing pschosomatic health were negative live change unit (- Leu), pschoticism, neuroticism, lie, and tendency to intervert of the character, driving kilometres, the number of years set for driving service, and psychiatric family history. The happening of the accident and error was related to some psychosomatic problems, neuroticism, more high degree of lie, greater age, driving kilometres, and the number of years set for driving service.

Objective: To explore the relationship between internet overuse and time management disposition in middle school students. Methods: Using Diagnostic Questionnaire for Internet Addiction (YDQ), Time Management Disposition Scale (TMDS), we investigated 2620 middle-school students.Results:The incidence rate of internet overuse among middle school students was 2.4 %. The difference of time management disposition between internet overuse group and control group was significant(129.7?25.3/150.0?28.5,t=4.27, P

Objective:To investigate the effect of group psychotherapy on middle school students with internet overuse(IOU).Methods:A total of 2620 middle-school students from four middle schools of Changsha City were surveyed using Diagnostic Questionnaire for Internet Addiction (YDQ), According to the YDQ criterion and clinical interview, 64 students who were diagnosed as Internet overuse. 29 middle school students with IOU from one regular middle school and one key middle school received group psychotherapy. 35 middle school students with IOU from the other two schools were taken as control(control group). All the students in both groups were assessed with Diagnostic Questionnaire for Internet Addiction(YDQ), Chen Internet Addiction Scale(CIAS), The Screen for Child Anxiety Related Emotional Disorders(SCARED), Strength and Difficulties Questionnaire(SDQ). Results: The scores 0f SCARD and SDQ subscale of emotional symptoms of experimental group decreased significantly (t=2.11～2.99,P