Objective To investigate the impact of nursing intervention on the prevention of deep venous thrombosis after orthopaedic surgery of lower extremities.Methods 104 patients were selected from our hospital after orthopaedic surgery of lower extremities were randomly divided into the control group and the observation group with 52 cases in each group,the control group was given normal routine care,the observation group received target-oriented nursing.The incidence of deep venous thrombosis was compared between the two groups.Results The rate of deep venous thrombosis in the observation group was significantly lower than that in the control group.Conclusions Through targeted nursing intervention,we can effectively prevent deep venous thrombosis after orthopaedic surgery of lower extremities,so it deserved promotion and application in clinic.

Objective:To construct a recombinant retroviral vector expressing pancreatic duodenal homeobox-1(PDX-1) gene and express the PDX-1 expression cassette in liver progenitor cells(LEPCs),so as to study the conversion of hepatic cells to pancreatic-like cells.Methods: The full length of PDX-1 gene was amplified by PCR and subcloned into pMSCVpuro vector.The pMSCV PDX-1 puro was then introduced into Phoenix package cells and the cell culture supernatant was used for Ping-Pong infection of another packaging cell line PT67 to obtain stable virus-producing cell line.Results: The pMSCV PDX-1 puro vector was successfully constructed and transfected into PT67 cells.The viral supernatants of PT67 cells efficiently infected LEPCs in vitro and the infected LEPCs stably expressed PDX-1 gene.Conclusion: The pMSCV PDX-1 puro vector and PDX-1 expressing LEPCs have been successfully constructed,laying a basis for studying the transdifferentiation of liver progenitor cells to pancreas lineage cells.

Objective:To construct a recombinant retroviral vector harboring MafA and to establish a liver epithelial progenitor cell(LEPC)line for stable expression of MafA.Methods:MafA was amplified by PCR and suncloned into pBMN-Z- IRES-Neo vector to obtain pBMH-MafA-Neo vector.After introducing the pBMN-MafA-Neo into Phoenix package cells,the cell culture supernatant was used to infect LEPCs.LEPCs stably expressing MafA gene were screened out.RT-PCR was used to detect the influence of MafA on the phenotype of LEPCs.Results:We successfully constructed pBMH-MafA-Neo vector and obtained LEPCs which stably expressed MafA.Expression of GK and GLUT2 genes in LEPCs-MafA cells was higher than that in the LEPCs.Conclusion:We have successfully obtained LEPCs which can stably express MafA,laying a basis for studying the differentiation of LEPCs into pancreas cells.

Objective Observing the clinical effect of Ligustrazine Phosphate Tablets combined with percutaneous intratumoral injection of lipiodol THP on advanced hepa-tocellular carcinoma.Methods83 patients with advanced hepatocellular carcinoma from January 2012 to September 2014 were analyzed.Double-blind, randomized method All patients were divided into the control group 41 cases and observation group 42 cases.The control group were treatedwith percutaneous transhepatic intratumoral injection of lipiodol THP, the observation groupwere given Ligustrazine Phosphate Tablets combined with percutaneous intratumoral injection of lipiodol THP to treat.After treatment, the two groups of patients with treatment efficacy, adverse reactions and Karnofsky and prothrombin time were analyzed.ResultsAftertreatment, the efficacy of the two groups of patients were compared, the results showed that patients in the observation group total effective rate of the control group (χ2=4.034),the difference was statistically significant (P<0.05);two groups adverse reactions in patients after treatment were compared, liver pain, blood toxicity, fever, gastrointestinal reactions similar proportion of patients, the difference was not statistically significant;Theproportion of patients in the observation group AFP<8.1ng/mL was significantly higher (χ2=4.338), the difference was statistically significant (P<0.05);patients were observed after treatment Karnofsky score was significantly higher, the difference was statistically significant (t=7.141, P<0.05);after two groups of patients were compared prothrombin time, the difference was not statistically significant.ConclusionHepatic arterial chemoembolization and percutaneous intratumoral injection of THP lipiodol emulsion on the basis of the combination of Ligustrazine Phosphate Tablets can effectively improve the efficacy of treatment, and has good security, which is a safe and effective methods of treatment should be to promote and use in clinical.

A representative nitrogen doped titanium dioxide sample (denoted as N-doped TiO_2) was prepared by treating nanotubular titanic acid (H_2Ti_2O_5·H_2O,NTA in short) in flowing NH_3 at an elevated temperature of 500℃ for 4 h.The sub-band structure within Eg of N-NTA-500 was determined by means of photoluminescence (PL) spectrometry and ultraviolet-visible light-near infrared (UV-Vis-NIR)diffusion reflectance spectrometry (DRS).Moreover,the photocatalytic activity of N-NTA-500 was evaluated by performing the redox reaction of propylene under the irradiation of alternate on-and-off visible light,using commercially available P-25 titania as a comparison.It was found that a large amount of single-electron-trapped oxygen vacancies (denoted as Vo~·) formed a sub-band within the Eg of N-NTA-500,contributing to extend the optical absorption of N-doped TiO_2 into visible light region.The sub-band width,the bottom energy above the top edge of valence band,and the top energy below the bottom edge of conduction band were determined to be 0.24 eV,1.79 eV,and 0.99 eV,respectively.Results and discussion:Each of the four PL spectra at an excitation wavelength of 360-390 nm can be deconvoluted into two bands.The peak positions of the two deconvoluted PL bands for each of the four PL spectra at λ_(ex)=360-390 nm are unchanged and located at 465 nm and 570 nm,respectively,implying that they are not originated from the back band-to-band transition but the e~--h~+recombination on two surface states.The band at 465 nm represents the emission on shallow surface state (2.66 eV above valence band edge),while that band at 570 nm represents the emission on deep surface state (2.17 eV above valence band edge).Moreover,the PL peak intensity decreases with increasing excitation energy,indicating that the photogenerated energy-enriched electrons are firstly relaxed to the bottom of the conduction band and then transferred to the surface state,and the larger the value of E_(ex),the more the energy loss.Therefore,it can be concluded that the excitation at λ_(ex)≤400 nm for N-NTA-500 is dominated by band-to-band transition,while E_g of the N-doped TiO_2 is identical to that of TiO_2 (anatase),I.e.,3.1 eV at 400 nm.The emission mechanism with respect to band-to-band transition of N-NTA-500 in an excitation wavelength range of 360-390 nm is depicted.

TBX20 gene, a member of the T-box gene family, plays an important role during heart development and is required for maintaining adult heart function.TBX20 can act as both transcriptional activator and transcriptional suppressor during the development of the heart, and shows complex spatiotemporal regulation.TBX20 is an important candidate transcription factor for the pathogenesis of congenital heart disease(CHD), and its mutation or expression change can lead to the occurrence of CHD.In this paper, the latest research progress of TBX20 gene in the field of heart development is reviewed from the relationship between TBX20 and CHD, the signaling pathway involved in heart development and its interactions with other cardiac transcription factors.

目的探讨康复训练配合针刺治疗脑卒中患者吞咽功能障碍的疗效。方法 87例脑卒中伴吞咽功能障碍者分为综合治疗组(56例)和对照组(31例)。综合治疗组进行针灸和康复训练 ,对照组仅进行内科常规治疗。结果综合治疗组与对照组在统计学上有非常显著性差异(P<0.01)。结论脑卒中吞咽功能障碍患者早期进行综合康复能促进功能恢复、减少鼻饲并发症。

Based on the results of oral glucose tolerance test( OGTT )and the levels of 1-h plasma glucose ( 1 hPG),793 subjects were classified into three groups:583 with NGTN ( normal 1 hPG in OGTT),127 with NGT1 H( higher 1 hPG in OGTT) and 83 with IGT( impaired glucose tolerance).NGT1H group had large waist circumference,higher body mass index,fasting plasma glucose( FPG),triglyceride,and lower high density lipoprotein-cholesterol than those of NGTN group.NGT1 H group had higher homeostasis model assessment insulin index ( 1.2 ± 0.6),lower homeostasis model assessment β3 ( HOMA-β ) (4.5 ± 0.7 ) and insulinogenic index (2.1 ±0.7) than those of NGTN group(0.5 ±0.6,4.8 ±0.7,2.7 ±0.9,respectively,all P ＜0.05 ).HOMA-β of NGT1 H group was higher than that of IGT group(4.5 ±0.7 vs.4.4 ±0.6,P ＜0.05 ).The results indicate that 1 hPG in OGTT may identify a condition of glucose metabolic abnormalities characterized by insulin resistance and reduced β-cell function.

Objective To evaluate the function of peripheral vessels, the change of ankle-brachial Index (ABI) and toe-brachial Index (TBI), and its related risk factors in elderly type 2 diabetic patients were observed. Methods Doppler frequency spectrum was used on lower limb of 128 type 2 diabetic patients and 103 non-diabetic people. ABI and TBI were calculated, other indexes including body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), hypersensitivity C-reactive protein(hs-CRP), triglyceride (TG), total cholesterol (TC), high density lipopretein (HDL), low density lipoprotein (LDL), fasting blood glucose(FBG), postprandial blood glucose (PPBG) and glycosylated hemoglobin (HbAlC) were measured. Results ABI and TBI were decreased in T2DM group as compared with control group (P<0.05), and hs-CRP was increased in T2DM group. With multiple stepwise regression, it showed that ABI and TBI was negatively related with age, SBP and T2DM duration, while ABI was positively correlated with HDL. In multiple logistic regression, cigarette, age, high blood pressure(HBP) and T2DM duration were independent risk factors with respective odds ratios of 1.94 (P<0.05), 2.12(P<0.01), 2.74(P<0.05) and 1.59(P<0.05), respectively. Conclusion Older age, T2DM duration, ciga-rette, hyperglycosema, HBP, low HDL and high bs-CRP play important roles in the occurrence and development of peripheral vascular dis-ease (PVD) in elderly type 2 diabetic patients. PVD of type 2 diabetic patients can be found earlier with Doppler diagnostic instrument.

AIM: To study the effect of peppermint oil on GSH、ATPase in rat liver-tissues and primary cultured rat hepatocyte. METHODS: The rat liver tissues were obtained to detect content of GSH and level of Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase after being administered peppermint oil orally at 24 % concentration of peppermint oil in 36 and 48 hours.Primary rat hepatocyte was separated and cultured,then peppermint oil and the serum containing that were respectively added level of LDH,ALT,AST in the supernatant fluid was respectively determined after incubating for 12,24 and 48 hours. RESULTS: The content of GSH and level of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)ATPase in liver tissues was low remarkably(P