Transmission electron microscopy, histochemstry, immunohistochemistry and morphometry were used to study ultrastructural changes, succinic dehydrogenase (SDH) activity and myoglobin (Mb )content of the rabbit's myocardium bleeding to death and to hemorrhagic shock. The results were as follows: the myocardium showed different degrees of ultrastructural changes; various degrees of decreasing of SDH activity and deletion of Mb. These changes gradually become more serious with lapse of time after shock. The mechanism of the changes and the significance in forensic medicine are discussed.

To study the pathological process and renal patho morphology of crush syndromed.The rabbit model of crush injury crush syndrome was established.Transmission electron microscopy(TEM),biochemistry,radioimmunoassay and immuohistochemistry were employed.The results demonstrated that the serum K + and BUN increased,The serum CO 2CP decreased after crush injury.The degeneration and necrosis of the epithelial cells of the renal tubule and myoglobin casts in the renal tubules were observed in crush syndrome,the serum K + increase was absent.Our results may be regarded as criteria for the diagnosis of the crush injury crush syndrome.The serum K + increase was the cause of death in early stage after crush injury.

Objective To investigate a method for postmortem diagnosis of the sudden death from hypersensitivity by detection of serum tryptase and IgE.Methods Venous blood samples were collected from 9 cases of sudden death from hypersensitivity,19 cases of death from other causes and 10 health living adult individuals.The serum levels of the mast cell tryptase(MCT)and IgE were detected with immunofluorescent enzyme assay and enzyme linked immunosorbent assay(ELISA)respectively.MCT in the lungs was detected by immunohistochemistical method.Results The serum level of both MCT and IgE in cases of sudden death from hypersensitivity were higher than those in health living adults and cases of death from other causes(P﹤0.01).However,there was significant difference(P﹥0.05)in the serum level of IgE and MCT between the health living individuals and cases of death from other cause.More cells containing MCT-positive granules were detected in the lung in cases of sudden death from hypersensitivity than in cases of death from other causes(P

Objective To explore the pathognomonic findings of anaphylactic shock in forensic medicine. Methods Animal model of anaphylactic shock was developed in guinea pigs. Substance P level in plasma was determined radioimmunoassay, and detected in laryngopharyneal tissue and the lungs by immunohistochemical SABC method followed by image analysis using BI - 2000 image analyzing system. Positive index (PI) was calculated. Results There was a statistically significant difference for substance P concentration in plasma between the guinea pigs with anaphylactic shock (131.01? 18.93pg/?l)and the control animals (87.70 ?7. 60pg/?l) (P

Objective To observe the dynamic changes in plasma coagulation indexes in the rabbit's amniotic fluid embolism. Method The experimental amniotic embolism model was established by injecting the amniotic fluid or placental extract-amniotic fluid through the rabbit's marginal vein of the ear. Blood samples were taken from the heart before and after infusion. Fibrinogen (FIB), prothrombin time (PT), thrombin time (TT), and activated partial thromboplastin time (APTT) were measured with coagulation analyzer. Results As compared with the sham operation group, FIB decrease significantly after infusion with amniotic fluid and placenta! extract mixed with amniotic fluid (P

Objective Carry out the mast cell tryptase (MCT) granule count in throat, lung and small intestine mast cells in persons died of anaphylactic shock.Method Control group: 10 cases died from severe brain injury. Research group:15 cases died from anaphylactic shock(group A)and 8 cases died of amniotic fluid embolism (group B). H & E staining and immunohistochemical staining were employed and the MCT granule count performed on the throat, lung and small intestine tissue.Results There was marked increase of MCT granule count in the throat (average MCT-positive number of group A 28.48 and of group B 24.35),lung (average MCT-positive number of group A 46.98 and of group B 43.50) and small intestine (average MCT-positive number of group A 48.23 and of group B 42.72) in specimens of group A and group B as compared with the control group (average number in throat 7.97,lung 12.94 and small intestine 20.25).Conclusion Tryptase granular count of MCT increased in throat, lung and small intestine tissue in persons died from anaphylactic shock and amniotic fluid embolism.

Objective To observe the number of pulmonary mast cell tryptase(MCT) after amniotic fluid embolism(AFE) in experimental rabbits.Method The experimental amniotic fluid embolism rabbits model was established by injecting the amniotic fluid(AF) or placental extracts mixed with amniotic fluid(PAF)into the marginal vein of the pregnant rabbits.Immunohistochemistry and microscopy were employed to observe the number of pulmonary MCT in AFE rabbits.Results A marked increase in MCT positive cells was observed in lungs in both AF and PAF groups(average cell number were 32 98 and 45 53respectively)as compared with the control group(average cell number was 11 25).Conclusion The results indicated that the number of MCT cells were marked increased in case of the AFE.

Objective To analyze the characteristics in the incarcerated inm ate’s death, investigate the m ain cause of death of the incarcerated inm ate and provide som e inform ation for forensic investigation. Methods The cases from the forensic m edical center of Shanxi Medical U niversity from 2005 to 2013 were selected. The statistical analysis w as perform ed by using the incarcerated inm ate’s gender, age, cause of death, m anner of death, and disease as the m arkers. Results There were 100 men, 5 w omen in the 105 incarcerated inm ates;the age range w as from 16 to 65 years;Inm ates were mostly died of nat-ural diseases, m ainly in the respiratory and cardiovascular diseases;the m ain unnatural death w as suicide w ith a rate of 54.5%. Conclusion Atpresent, most incarcerated inm ate’s death are due to natural dis-eases. The prison should im prove incarcerated inm ate’s lives, w ork and health care conditions, and strengthen supervision of law enforcement.

Objectives To detect the clinical significance of high sensitive C-reactive protein (hs-CRP) and immune function in children with Mycoplasma pneumoniae pneumonia (MPP). Methods 103 children with MPP, 47 cases of systemic inflammatory response syndrome (SIRS group), 56 cases of non-systemic inflammatory response syndrome (non-SIRS group) were recruited. 26 healthy children served as the control group. ELISA was used to detect the level of serum hs-CRP, immune in-dexes, IgG, IgA, and IgM, Cellular immune CD3+, CD4+, CD8+, CD4+/CD8+. Results The level of serum hs-CRP、IgG、IgM and CD8+in control group were significantly lower than those in non-SIRS group and SIRS group (P<0.05). The level of IgA、CD3+, CD4+, CD4+/CD8+in control group were significantly higher than those in non-SIRS group and SIRS group (P<0.05). The level of serum hs-CRP、IgG in SIRS group were significantly higher than those in non-SIRS group (P<0.05). The level of IgA、CD3+、CD4+、CD4+/CD8+in SIRS group were significantly lower than those in non-SIRS group. There was no significant difference in non-SIRS group and SIRS group of IgM and CD8+(P>0.05). The level change of serum hs-CRP were positively related with IgG (r=0.66,P=0.001) and were negatively related with IgA、CD4+、CD4+/CD8+(r=0.79, 0.67, 0.82, P all were<0.05) in chil-dren with MPP. Conclusion Children with MPP have Immunity function( including humoral immunity and cellular immunity) disorder which is related to the disease status. The level of hs-CRP could be an anpation index for the severity and immune func-tion of the children with MPP.

BACKGROUND: The occurrenceand development of rheumatoid arthritis were strongly associated with unbalance proliferation and apoptosis of synovial cells and lymphocytes. Some synovial cell apoptosis was abnormal. OBJECTIVE: To observe effects of heterogenic double umbilical cord blood stem cell transplantation on Bcl-2 and Bax expression in mice with type Ⅱcollagen-induced arthritis. METHODS: C57BL/6(H-2b) mice were induced with Freund's complete adjuvant and type Ⅱcollagen to establish mouse models of type Ⅱcollagen-induced arthritis.At 2days after secondary immunity incubation, mice were injected with saline via tail vein in the model and normal control groups. Umbilical cord blood hemopoietic stem cells were injected into mouse tail vein in the UBSCs transplantation groups (single dose:2×106/50 g; double dose: 1×106/50 g each, totally 2×106/50 g). Mice were intragastrically administrated methotrexate in the methotrexate positive control group, 0.017 5 g/kg once, once every 5 days, totally six times. RESULTS AND CONCLUSION: Joint tissue below knee and elbow was obtained at42 days following transplantation. Histopathology displayed that smooth and glossy articular surface, no inflammatory cell infiltration in the synovial layer and normal chondrocytes in the normal control group. Hyperplasia, a lot of inflammatory cell infiltration and damaged cartilage surface were visible in the model group. Slight hyperplasia and a little inflammatory cell infiltration were detectable in the methotrexate positive controlgroup and single UBSCs transplantation group. Double UBSCs transplantation group exhibited smooth and glossy articular cartilage surface, no damage, a little inflammatory cell infiltration. Immunohistochemistry demonstrated that Bcl-2 and Bax expression was lower in the double UBSCs transplantation group compared with single UBSCs transplantation group (P < 0.05). Compared with normal control group, no significant difference was detected (P > 0.05). Results suggest that double umbilical cord blood stem cells can induce synovial cell apoptosis in mice with type Ⅱcollagen-induced arthritis in a certain number and action time, and protect synovial membrane against damage.