Objective To investigate the different pretreatments of Caoshanhu extract and their effects on the downstream process,membrane separation.Methods Based on the index related to isofraxidin,the different pretreatment methods of Caoshanhu extract were evaluated and their effects on membrane separation downstream were analyzed by the comparison of different pretreatments and further orthogonal test of ZTC_(1+1) Ⅲ,chitosan,and(KAl(SO_4)_2?12H_2O).Results After the pretreatment of microfiltration,both the best membrane flux and the highest percentage of isofraxidin filtered through the membrane were obtained and the time used was the least,and the ratio of isofraxidin to insoluble substances was tiptop in all pretreatment methods in comparison to other methods,such as ZTC_(1+1) Ⅲ,chitosan,and(KAl(SO_4)_2?12H_2O) methods.Conclusion Microfiltration is a better pretreatment method in preparation of Caoshanhu extract.

Objective To determine the content of luteolin in Callicarpa nudiflora Hook.et Arn..Methods HPLC was performed with chromatographic conditions as follows:Alltima C18 column(250 mm? 4.6 mm,5 ? m)with column temperature at 25 ℃,the mobile phase of methanol-water-phosphate(55:45:0.4),flow rate at 1 mL/min,and the detection wavelength at 348 nm.Results The linearity of luteolin was good in the range of 0.032 46～ 0.129 84 ? g(r=0.999 5).The average recovery was 99.52 % and RSD was 1.58 %.Conclusion The method is simple,feasible and reproducible,and can be used to control the quality of Callicarpa nudiflora Hook.et Arn..