OBJECTIVE To investigate the clinical practicability of inducible clindamycin resistance and to evaluate the clinical value of cefoxitin disk diffusion method in detecting the mecA gene of meticillin-resistant Staphylococcus(MRS). METHODS Resistant phenotypes were classified with erythromycin and clindamycin discs using the D test method and the cefoxitin disk diffusion method in detecting mecA gene of the staphylococcus. RESULTS Of all 390 Staphylococcus strains,218 strains(55.9 %) were resistant to erythromycin and clindamycin(cMLS).Seventy strains(17.9 %) which were resistant to erythromycin but susceptible to clindamycin showed the positive D test results(iMLS),79 strains(20.3 %) resistant to erythromycin but susceptible to clindamycin showed the negative D test results(MS).From 390 strains of clinical isolated staphylococcus,which using the cefoxitin disk diffusion method,272 strains were MRS. CONCLUSIONS Using the D test method to investigate the inducible resistance to erythromycin and clindamycin in the Staphylococcus strains,which could help the doctors choose correctly the antibiotics such as macrolides and clindamycin.At the same time,cefoxitin disk diffusion method can be used reliably by the clinical laboratory in detecting and conforming MRS.

OBJECTIVE: To detect pathogenic variant of the FGD1 gene in a boy with Aarskog-Scott syndrome. METHODS: Genetic variant was detected by high-throughput sequencing. Suspected variant was verified by Sanger sequencing. The nature and impact of the candidate variant were predicted by bioinformatic analysis. RESULTS: The child was found to harbor a novel c.1906C>T hemizygous variant of the FGD1 gene, which has led to conversion of Arginine to Tryptophane at codon 636(p.Arg636Trp). The same variant was found in his mother but not father. Based on the American College of Medical Genetics and Genomics guidelines, the c.1906C>T variant of FGD1 gene was predicted to be likely pathogenic(PM1+PM2+PM5+PP2+PP3+PP4). CONCLUSION The novel c.1906C>T variant of the FGD1 gene may underlay the Aarskog-Scott syndrome in this child. Above finding has enabled diagnosis for the boy.
Child ; Dwarfism ; Face/abnormalities* ; Genetic Diseases, X-Linked ; Genitalia, Male/abnormalities* ; Guanine Nucleotide Exchange Factors/genetics* ; Hand Deformities, Congenital/genetics* ; Heart Defects, Congenital ; Humans ; Male ; Mutation

Objective To explore the effect of the whole course nursing intervention on the clinical efficacy and postoperative complications in patients with colonic surgery .Methods Seventy patients with colonic surgery were chosen and divided into the control group and the observation group according to the random number table, each with 35 cases.The control group received the routine nursing after the operation , and the observation group received the whole course nursing intervention .The clinical efficacy and the incidence rate of postoperative complications were observed in two groups .Results The total effective rate of treatment was 88.57%in the observation group, and was better than 65.71% in the control group, and the difference was statistically significant (χ2 =5.185 2, P <0.05 ).The incidence rate of postoperative complications was 17.14%in the observation group, and was lower than 31.43% in the control group, and the difference was statistically significant (χ2 =4.480 0,P <0.05).Conclusions The whole course nursing intervention in patients with colonic surgery can effectively improve the clinical efficacy , and reduce the incidence of postoperative complications , and improve the prognosis , and accelerate the speed of rehabilitation , and improve the patients ’ quality of life .

Objective:To develop a health behavior guidance program for stroke rehabilitation patients based on the adaptive leadership framework (ALF) .Methods:Relevant stroke health behavior guidelines were systematically retrieved, screened, included, and recommendations extracted from domestic and international websites. The initial draft of the health behavior guidance program for stroke rehabilitation patients was developed in light of the ALF. From January to March 2022, the draft was refined through two rounds of Delphi expert consultation, resulting in the finalized health behavior guidance program.Results:A total of 10 guidelines were included, and 73 guideline recommendations were extracted. These recommendations were categorized into six dimensions and 25 specific items. In both rounds of expert consultation, 15 questionnaires were distributed and all were returned, yielding an effective recovery rate of 100.00%, with an expert authority coefficient greater than 0.8. Based on expert feedback and group discussions, the final program included eight dimensions and 31 items, adding sleep management and regular health consultations to the original six dimensions.Conclusions:The health behavior guidance program for stroke rehabilitation patients, based on the ALF, introduces a new perspective on nursing by reasonably dividing adaptive and technical problems. This approach aligns with clinical practice in China, providing scientific and practical value for promoting health behaviors and offering a new perspective for health education in stroke patients.

Objective This paper aims to address the challenges of hospital equipment management by implementing the life cycle management system in the medical laboratories of public hospitals.Methods Based on the three principles of standard-ization,refinement,and dynamism,this paper conducted data tracking and managed equipment analysis with specifications dur-ing the processes of planning,discussion,procurement,acceptance,operation,maintenance,performance evaluation,scrap-ping,and updating.Results After implementing life cycle management,the average risk level significantly decreased compared to pre-implementation(P＜0.05).Budget implementation efficiency increased by 14.53％from 2019 to 2023.The investment payback period was controlled within 2 years.Conclusion The application of the life cycle management system to equipment management in medical laboratories of public hospitals is of great significance for the high-quality development of hospitals.