Objective To explore the association of lipid metabolism and bomocysteine with hypertensive disorders in pregnancy and observe the inflammatory reaction and the pathological change of placenta.Methods We conducted a prospective randomized placebo-controlled study.Seventy-four cases with hypertensive disorders in pregnancy were recruited as the experimental group and 77 cases of normal pregnant women were recruited as matched control group from Aug.2008 to Mar.2010.The relationship between lipid metabolism and homocysteine was analyzed.Results There was significant difference between the two groups in body-mass index before pregnancy(29.03±4.52 vs.23.99±5.90,t =5.88),cholesterol[(6.16±1.48)mmol/L vs.(5.01±1.05) mmmol/L,t =5.52],low density lipoprotein[(3.46±1.35) mmol/L vs.(2.26±0.86) mmol/L,t =6.54]and C-reactive protein[(29.04±14.90) mmol/L vs.(15.48±4.57) mmol/L,t =7.62](P ＜ 0.05).No significant difference was found on triglycerides[(3.29±1.03) mmol/L vs.(3.49±1.37) mmol/L],high density lipoprotein[(1.84±0.40) mmol/L vs.(1.88±0.35) mmol/L],homocysteine [(8.77±2.65) mmol/L vs.(8.40±2.03) mmol/L]and neonatal weight[(3547±519)g vs.(3431±461)g](P ＞ 0.05).Significant difference exists in placenta pathology of infarction(54.55％ vs.31.04％),villi dysplasia,fit nodules form and hypoxia(65.46％ vs.39.66％)(P ＜0.05).Conclusion We should detect lipid,homocysteine and C-reactive protein by dynamic combinational ways,pay more attention to women with high BMI before pregnancy,and value placenta pathology research on hypertensive disorders in pregnant women.This has high clinical significance in revealing the etiology of hypertensive disorders in pregnant women and improving pregnant outcomes.

OBJECTIVE: The success of joint prosthesis greatly depends on the materials, which should be a perfect combination of physics, chemistry, biomechanics and clinical sciences in choice of them. In addition, the effect of manufacture technology can not be neglected. So, it is significant to probe into the systematic basis in materials selection of joint prosthesis.DATA SOURCES: A computer-based search was conducted in Pubmed database for articles about joint prosthesis published before April 2006 with the key words of "artificial joint materials, biomaterial", and the language was limited to English. Meanwhile, Chinese Journal Full-text Database (CJFD) and Wanfang database were looked for relevant literatures published before April 2006 with the key words of "artificial joint material, biomedical material", and the language was limited to Chinese. Besides,the standards for materials were searched in Chinese service net for standard consultation with the key words of "implants for surgery" in both Chinese and English. At the same time, relevant books were also manually searched.STUDY SELECTION: Data were checked in the first trial, and related articles according to the criteria of research were looked for the full text.Inclusion criteria: ① Requirements for artificial joint material. ② Relevant standards for artificial joint of surgical implants. Exclusion criteria: Repetitive studies were excluded.DATA EXTRACTION: A total of 390 domestic and overseas articles,which were in relation to the mechanical properties of artificial joint material and bone and measurement of the overall dimensions of knee joint,were collected. There were still 242 relevant standards, while the repetitive studies and similar researches were excluded. Ten typical articles and 25 standards in collection were summed up and reviewed.DATA SYNTHESIS: ①Close combination of physics, chemistry, biomechanics and clinical sciences is sufficiently embodied in the selection of the materials. ② Two aspects must be taken into consideration: Requirements for function of the implant and the responses inside the human body.③ Main types of materials for joint prosthesis: biomedical metallic material, biomedical inorganic material of nonmetal, bioceramic, biomedical compound materials and so on. All kinds of materials that have been adopted should accord with the international or state or trade standards.④ The final determination of materials should meet the comprehensive requirements of standards, laws and regulations, as well as strategic targeting. CONCLUSION: Based on engineering, the fundamental principles of materials selection for joint prosthesis are illustrated from the following angles: clinical requirements for materials of replacement, attentions in joint prosthesis design, commonly used biomedicine materials performance, requirements of product registration and laws & regulations related to the materials, the strategy in the choice of material standard etc. As a result, a valuable system approach is provided for joint-prosthesis designers to select materials.

Objective To summarize the perioperative nursing experience of microsurgical denervation of the spermatic cord to treat idiopathic chronic orthialgia.Methods We retrospectively analyzed the clinical record data of two patients with idiopathic chronic orthialgia,who received microsurgical denervation of the spermatic cord in our hospital,including pre-and postoperative symptom scores,evaluation of mental state and wound nursing.Results Both patients got complete pain relief and were discharged one week after operation.No would infection and mental fluctuation was noted.Conclusions The reasonable individual perioperative nursing is an elemental component of recovery for patients with chronic orthialgia who experienced microsurgical management.

Objective To explore the effect of head nurses′tutoring and commenting on nursing ward round. Methods The nursing ward rounds performed during August 2013 to July 2014 were assigned as the control group and those nursing ward rounds during August 2014 to August 2015 as observation group: in the former group routine nursing ward rounds were done once a week and in the latter the rounds were performed by head nurses′tutoring and commenting once every week. The two groups were compared in terms of examination performances of nursing ward rounds, doctors′ and nurses′ satisfaction degree, nurses′ and student nurses′evaluation on the ward rounds and their critical thinking ability. Results The examination performances and the satisfaction degree in the observation group were significantly higher than those of the control group (P<0.05). The nurses in the observation group thought the head nurse′s commenting at ward rounds could be more effective than those from the control group in their improvement of theoretical knowledge and clinical ability (P<0.05). In terms of critical thinking by California Critical Thinking Disposition Inventory-Chinese Version), the total score and the scores on its dimensions in the observation group were significantly higher than those of the control group. Conclusion The nursing ward rounds by head nurses′tutoring and commenting can help the nurses to strengthen their ability in nursing ward rounds and improve their theory and clinical practice so that the nursing quality is improved.

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Objective:To investigate relationships between nasal parasitism by Demodex mites and nasal skin microbiome in patients with rosacea. Methods:From May 2017 to June 2019, 14 patients with rosacea, including 8 with early-stage rosacea and 6 with intermediate-stage rosacea, and 14 human controls with healthy facial skin were collected from Department of Dermatology, Shunde District Center for Prevention and Cure of Chronic Disease of Foshan City. Microbial samples were collected from the nasal alar and nasolabial folds of the subjects. Then, DNA was extracted from the samples, and subjected to metagenomic sequencing and bioinformatics analysis. Relative abundance of strains was estimated by using composition ratios of Demodex mites and microbial reads. Shannon index was calculated to evaluate α diversity of microbiome, and principal component analysis (PCA) was performed to assess β diversity based on relative abundance of microbial species. Enumeration data were compared by using two-independent-sample t test, and relationships between nasal Demodex mites and skin microbiome were analyzed by Pearson correlation analysis. Results:The relative abundance of nasal Demodex mites was significantly higher in the rosacea group (1.647% ± 0.389%) than in the healthy group (0.448% ± 0.089%, t = 2.92, P = 0.007) . The relative abundance of Demodex mites was negatively correlated with the relative abundance of bacteria ( r = -0.95, P < 0.001) , and positively correlated with the relative abundance of fungi ( r = 0.76, P < 0.001) . The Shannon indices of nasal bacterial and fungal communities were significantly higher in the rosacea group (0.91 ± 0.17, 1.261 ± 0.045, respectively) than in the healthy group (0.47 ± 0.12, 0.549 ± 0.071, t = 2.17, 8.48, respectively, both P < 0.05) ; PCA showed that the β diversity of bacterial communities significantly differed between the rosacea group and healthy group ( t = 2.32, P = 0.029) , while no significant difference in the β diversity of fungal communities was observed between the two groups ( t = 0.82, P = 0.461) . In addition, the relative abundance of Demodex mites was significantly higher in the patients with intermediate-stage rosacea than in those with early-stage rosacea ( t = 6.56, P < 0.001) ; there was no significant difference in the Shannon indices of nasal bacterial or fungal communities between the two patient groups (both P > 0.05) ; PCA showed that the β diversities of bacterial and fungal communities significantly differed between the two patient groups (both P < 0.05) . Conclusion:Parasitism of Demodex mites on the nasal skin may affect nasal microbial community structure.

Objective: To evaluate the effect of age and gender on skin microbiome on the face of healthy adults by metagenomic sequencing. Methods: From June 2017 to June 2018, 36 adult volunteers with healthy facial skin were enrolled from the Department of Dermatology, Center for Prevention and Treatment of Chronic Diseases of Shunde, including 16 young volunteers (9 males and 7 females) aged 24-31 (27.1 ± 1.3) years and 20 senior volunteers (10 males and 10 females) aged 61-84 (75.8 ± 2.2) years. Skin microbe samples were obtained from the cheek of volunteers, and DNA was extracted from these samples and subjected to metagenomic sequencing and bioinformatic analysis, so as to evaluate the effect of age and gender factors on microbiota on the healthy facial skin of adults. Statistical analysis was carried out by using two independent-sample t test, Wilcoxon rank sum test and Pearson correlation analysis. Results: The senior group showed significantly higher α diversities of bacterial and fungal communities on the facial skin (Shannon index: 0.98 ± 0.07, 1.11 ± 0.05 respectively) compared with those in the young group (0.72 ± 0.09, 0.81 ± 0.05 respectively; t = 2.201, 3.836, P = 0.035, < 0.001 respectively) . Principal component analysis revealed that age could significantly affect β diversities of bacterial and fungal communities on the facial skin (t = 6.991, 11.591 respectively, both P < 0.001) . There were no significant differences in α diversities of bacterial and fungal communities between males and females (Shannon index: bacteria, 0.83 ± 0.08 vs. 0.92 ± 0.09; fungi, 0.92 ± 0.06 vs. 1.04 ± 0.05; t = 0.801, 1.332 respectively, both P > 0.05) . Gender factor could only affect the β diversity of bacterial communities (t = 2.149, P = 0.020) , but not the β diversity of fungal communities (t = 0.439, P = 0.663) . Moreover, the activity of metabolic pathways in bacterial and fungal communities was significantly lower in the senior group than in the young group (t = 1.995, 2.464, P = 0.020, 0.025, respectively) , while gender factor did not affect the activity of metabolic pathways in bacterial and fungal communities (t = 0.895, 0.483, P = 0.378, 0.631, respectively) . According to the relative abundance of different bacteria and fungi between the senior group and young group, Pearson correlation analysis showed positive or negative correlations between some fungi and bacteria, between some bacteria and bacteria, as well as between some fungi and fungi. Conclusion Age factor, but not gender, markedly affects α and β diversities of, and activity of metabolic pathways in bacterial and fungal communities on the healthy face of adults.