Objective　To observe the effect of superoxide dismutase(SOD)-compound on bacterial ulceration in rabbit's eyes and explore its mechanism.Methods　Amikacin and SOD-compound etc.were added to agar inoculating PS.Pyocyaneus and the diameter of inhabiting circles were measured.The rabbit model of bacterial corneal ulcer was created and then treated with amikacin.SOD-compound was used as an adjunctive treatment while inactive SOD-compound as a control.The clinical manefestation and its pathological change were observed and the amlonyldialdehyde (MDA) in the ulcer was assayed.Results　In vitro experiment indicated that SOD-compound had no anti-bacterial effect.In rabbit corneal ulcer treated with SOD-compound,the clinical manefestation and pathological change was slighter,the MDA was lower.Conclusion　In rabbit's corneal ulceration,free radical and lipid peroxidation take part in the tissue damage.SOD-compound can contradict this effect through eliminating excessive free radical and anti-lipid peroxidation.Therefore,SOD-compound has protective effect on corneal physiology and histological structure in bacterial corneal ulceration.

The central corneal thickness (CCT) in age 48 years or less of Chinese was characterized and its relationship with gender, age, refraction and intraocular pressure (IOP) was investigated. Right eyes of 1669 participants were included in this study (880 men, 52.7% and 789 women, 47.3%). Mean age of the samples was 23.8 +/- 5.9 years. After the examination of corneal topography and refraction, Goldman applanation tonometry was carried out by one physician. Tonometric values were the mean of three consecutive readings. Subsequently, another physician carried out ultrasonic pachymetry with the DGH 2000 AP ultrasonic pachymeter. Six measurements were made at the center of the cornea of each eye. The mean value was used for analysis. The results showed that mean CCT of male participants was 551.33 +/- 34.62 microm, 5.79 microm more than that of female participants. Linear regression analyses revealed that CCT was negatively related with age only in female and no association was found between refractive status and CCT. IOP was positively related to CCT, and there was a difference in IOP of 1.5 mmHg (1 mmHg = 0.133 kPa) per 100 microm difference in CCT. Ocular hypertension group was prone to have thicker cornea than average. The results indicated that in adult Chinese CCT tended to decrease with aging in female only. IOP measured by Goldmann tonometry was positively related with CCT so that CCT should be measured along with IOP.
Age Factors ; China ; Cornea/*anatomy & histology ; Corneal Topography ; Intraocular Pressure ; Reference Values ; Sex Factors ; Tonometry, Ocular

To evaluate the inhibiting effect of Homoharringtonine (HHT) on the corneal haze after excimer laser photorefractive keratectomy (PRK) in rabbits. 18 healthy rabbits which underwent PRK were randomly divided into three groups (A, B and C). The refractive degree of ablation was - 10.0DS in each group. Group A was locally treated with a piece of filter paper soaked with 1 mg/mL HHT for 5 min, and then the entire cornea was repeatedly irrigated with balance solution; Group B was dropped with 0.1 mg/mL HHT after PRK for 3 months; Group C was the control group. Corneal haze, histopathology, response, ect. were investigated. The corneal haze was significantly less in group A, while the difference between group B and group C was insignificant. Keratocytes and fibrocytes in corneal stroma were more active up to 3 months in group B and group C. Intraoperative use of topical HHT can reduce corneal haze after PRK in rabbits.
Corneal Opacity/etiology ; Corneal Opacity/*prevention & control ; Endothelium, Corneal/pathology ; Harringtonines/*administration & dosage ; Myopia/*surgery ; Photorefractive Keratectomy/*adverse effects ; Random Allocation

The central corneal thickness (CCT) in age 48 years or less of Chinese was characterized and its relationship with gender, age, refraction and intraocular pressure (IOP) was investigated.Right eyes of 1669 participants were included in this study (880 men, 52.7 % and 789 women,47.3 %). Mean age of the samples was 23.8±5.9 years. After the examination of corneal topography and refraction, Goldman applanation tonometry was carried out by one physician. Tonometric values were the mean of three consecutive readings. Subsequently, another physician carried out ultrasonic pachymetry with the DGH 2000 AP ultrasonic pachymeter. Six measuremen ts were made at the center of the cornea of each eye. The mean value was used for analysis. The results showed that mean CCT of male participants was 551.33± 34. 62 μm, 5.79 μm more than that of female participants. Linear regression analyses revealed that CCT was negatively related with age only in female and no association was found between refractive status and CCT. IOP was positively related to CCT, and there was a difference in IOP of 1.5 mmHg (1 mmHg=0. 133 kPa) per 100 μm difference in CCT. Ocular hypertension group was prone to have thicker cornea than average. The results indicated that in adult Chinese CCT tended to decrease with aging in female only. IOP measured by Goldmann tonometry was positively related with CCT so that CCT should be measured along with IOP.

To evaluate the inhibiting effect of Homoharringtonine HHT) on the corneal haze after excimer laser photorefractive keratectomy (PRK) in rabbits. 18 healthy rabbits which underwent PRK were randomly divided into three groups (A, B and C). The refractive degree of ablation was -10. 0DS in each group. Group A was locally treated with a piece of filter paper soaked with 1 mg/mi HHT for 5 min, and then the entire cornea was repeatedly irrigated with balance solution;Group B was dropped with 0.1 mg/mL HHT after PRK for 3 months; Group C was the control group. Corneal haze, histopathology, response, ect. were investigated. The corneal haze was sig nificantly less in group A, while the difference between group B and group C was insignificant.Keratocytes and fibrocytes in corneal stroma were more active up to 3 months in group B and group C. Intraoperative use of topical HHT can reduce corneal haze after PRK in rabbits.