1.Research Progress in Seeding Breeding of Paridis Rhizoma
Min LUO ; Juan LI ; Wenwei ZHANG ; Caifu DENG ; Qiusheng TAN
Chinese Journal of Information on Traditional Chinese Medicine 2016;(1):120-123,124
Paridis Rhizoma is a rare Chinese herbal medicine with variety of medicinal values. In recent years, the demand for Paridis Rhizoma has increased gradually. Artificial cultivation has met difficulties, while exploitation of wild resources was caught in a vicious circle and has overdrawn seriously. So it is of great significance to enhance the protection of Paridis Rhizoma, carry out basic research, in order to solve problems in seeding breeding, promote artificial cultivation to meet the market need and achieve sustainable development and supply. This article reviewed the status qua of seedling breeding of Paridis Rhizoma, including seed breeding, tuber breeding and tissue culture, with a purpose to standardize planting of resource conservation and utilization of Paridis Rhizoma.
2.Effects of Various Processings on Quality of Angelica dahurica
Qiusheng TAN ; Wenwei ZHANG ; Ling LI ; Caifu DENG ; Shun LUO ; Chuan LUO ; Zhongqiang WEI ; Meiquan YANG
Chinese Journal of Information on Traditional Chinese Medicine 2013;(7):49-51,52
Objective To study the quality difference of various processing methods about Angelica dahurica. Methods Samples were selected from different places, clean silt by washing and brushing, cut slices and cut blocks in equal division, dried and comminution, to determine total ash, ethanol thermal extract, imperatorin content and HPLC fingerprint similarity. Results The content of total ash was the lowest in “washed”and “washed & cut slice” sample, dilute ethanol thermal extract was the highest in“cut blocks”and“washed&cut blocks”sample, imperatorin content was the highest in“non-washed”and“non-washed & cut slice” sample. Conclusion Washed and cut process is not suitable in place of origin about Angelica dahurica.
3.A Study on HPLC Characteristic Spectrum of Cortex Moutan in Dianjiang County
Caifu DENG ; Qiusheng TAN ; Ling LI ; Xingyu REN ; Min LUO ; Wenwei ZHANG ; Mingliang SHEN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(12):69-71,72
Objective To establish an analytical method of chromatographic fingerprint of cortex moutan in Dianjiang County through HPLC;To provide reference for the quality control and general evaluation. Methods HPLC with Shim-pack VP-ODS C18 column (4.6 mm×250 mm, 5μm) was used;paeonol was taken as standard;the mobile phase was MeOH-H2O with liner gradient eluation;the delection mavelength was at 274 nm for paeonol and 230 nm for paeoniflorin;the flow rate was 1.0 mL/min. Fourteen batches of samples were analyzed to establish the fingerprint with paeonol and paeoniflorin as reference. Results The results of the 14 batches of samples and similarity evaluation showed that the similar degrees to the 10 batches among the 14 samples were between 0.968-0.998, which illustrated the good similar degrees among samples. Conclusion The established fingerprint with characteristics of stability, good reproducibility and simplicity, can be used for the quality evaluation and control of cortex moutan.