Objective: To compare the effecte of removing plaque by two toothbrushing methods. Methods: Forty subjects were chosen to take part in the randomized、examiner-blind、crossover study. Results: Modified Bass method has better effect of removing plaque, especially that at the central margin than Vertical method(P

?Objective:To investigate the mandibular growth in children with mixed dentition. Methods:Nineteen female children were yearly observed with cephalometric film in 4 successive years.The study began in 10 children at 7 years old and in another 9 at 9 years old. There was the same observation period (9 10 years old) in the two groups, therefore their results could be combined to analysis with mixed longitudinal methods. All data were analysed with SPSS 8.0 software.Results:Generally,Ar Po and Go Po increased significantly ( P 0.05 ).Conclusion:In mixed dentition, the mandibular depth growth is greater than its height growth in children with normal face.

OBJECTIVEThe aim of this study was to find the laws of angular changes of 5 cephalometric planes in children with mixed dentitions.

METHODSNineteen female children were annually examined with X-ray, and the cephalometric analysis was performed in continuous 4 years. Of these children, 10 children were investigated from the age of 7 years old, and the other 9 children from 9 years old. The two groups were longitudinally studied. All of the children were observed almost at the same age (9-10 years old) in the two groups, and the results were analyzed with mixed longitudinal methods. All data were analyzed with SPSS 8.0 software.

RESULTSIn the observation period, the angular relationship of FH to BaN remain constant among 5 cephalometric planes, and FH to SN was almost in the same situation.

CONCLUSIONIn mixed dentition, the angular relationship of FH, BaN and SN barely change.

Cephalometry ; Child ; Dental Occlusion ; Dentition, Mixed ; Face ; anatomy & histology ; Female ; Humans ; Longitudinal Studies ; Maxillofacial Development ; Radiography ; Reference Values ; Skull ; anatomy & histology ; diagnostic imaging

OBJECTIVETo isolate and culture bovine cementum-derived cells and investigate their biological properties.

METHODSCulture of primary bovine cementoblast (CB) were established from new-born bovine teeth. Cementum was manually dissected, fragmented, and digested twice with collagenase. Following a thorough wash to remove liberated cells, the remaining cementum fragments were plated and cultured in RPMI1640 containing 10% FCS and 1.5% Hepes. The cells in culture were identified using immunocytochemistry by expression of cementum attachment protein (CAP), osteocalcin (OCN) and alkaline phosphatase (ALP). ALP activity was measured by modified Gomori staining.

RESULTS(1) The cells in culture possessed the typical morphology of CB and there were no obvious change of the cell morphology up to 5th passage; (2) Cells in culture exhibited alkaline phosphatase activity and were also positive to CAP, OPN and ALP immunohistochemistry staining.

CONCLUSIONThe cells isolated and cultured in this experiment possess the properties of CB. This enables us to further observe its biological characteristics during cementogenesis and periodontal regeneration.

Alkaline Phosphatase ; metabolism ; Animals ; Cattle ; Cell Separation ; methods ; Cells, Cultured ; Dental Cementum ; cytology ; enzymology ; Immunohistochemistry

OBJECTIVETo find the laws of vertical growth of craniofacial complex in children with mixed dentition.

METHODS19 female children were annually observed with cephalometric film in 4 successive years, of whom 10 started at 7 years while the others at age of 9. The two groups were longitudinally studied. All data were analyzed by SPSS 8.0 software.

RESULTSIn the observation period, the height of most craniofacial regions continually grew. In anterior face, the middle facial height increased most (increased 4.7 mm from 7 to 10 years old, P < 0.01; increased 3.9 mm from 10 to 12 years old, P < 0.01), while the lower facial height nearly unchanged (increased 1.3 mm from 7 to 10 years old, P > 0.05; increased 1.4 mm from 10 to 12 years old, P > 0.05). Some other regions had their own growing characters.

CONCLUSIONSOnce the occlusal relationships of permanent incisors are established, it is difficult to control the anterior lower facial height with orthodontic treatments.

Cephalometry ; Child ; Child Health Services ; Dental Care ; methods ; Dental Occlusion ; Face ; anatomy & histology ; Female ; Humans ; Longitudinal Studies ; Maxillofacial Development ; physiology ; Skull ; anatomy & histology

OBJECTIVETo evaluate the effects of local-delivery of 25% metronidazol gel and mechanical cleaning using ultrasonic carbon fiber tip on dental implants with peri-implantitis.

METHODS27 implants with peri-implantitis were randomly assigned to receiving either 25% metronidazol gel treatment or carbon fiber tip ultrasonic scaling. All parameters including plaque index (PLI), probing depth (PD) of pocket, sulcular bleeding index (SBI), and BANA enzyme analysis were measured at baseline, 1, 2, 6 and 12 weeks after treatment.

RESULTSStatistically significant decrease (P < 0.05) in SBI, BANA test and PLI occurred in both treatment groups at all time intervals compared to baseline. PD had a decreasing tendency in both groups, but only metronidazole group reached statistically significant level (P < 0.05) at 2 and 6 week intervals compared to baseline. None of the treatment modalities produced any side effects on the implant and peri-implant tissues.

CONCLUSIONSBoth 25% metronidazol gel and mechanical cleaning using ultrasonic carbon fiber tip can be safely and effectively used in the treatment of peri-implant diseases.

Adult ; Anti-Infective Agents ; therapeutic use ; Carbon ; Dental Implants ; adverse effects ; microbiology ; Female ; Humans ; Male ; Metronidazole ; therapeutic use ; Middle Aged ; Periodontitis ; etiology ; therapy ; Time Factors ; Treatment Outcome ; Ultrasonics

OBJECTIVESTo study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).

METHODSGCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.

RESULTSThe first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.

CONCLUSIONSCalprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.

Amino Acid Sequence ; Electrophoresis, Polyacrylamide Gel ; Gingival Crevicular Fluid ; chemistry ; Humans ; Leukocyte L1 Antigen Complex ; Periodontium

OBJECTIVETo investigate the timing and location of the expression of bone sialoprotein (BSP) and osteopontin (OPN) in developing dental tissues of rats.

METHODSEvery three neonatal rats were sacrificed at day 1, weeks 1, 2, 3, 5 and 8. The mandibles were dissected and the first molar and the surrounding tissue were fixed, then demineralized with 15% EDTA. Immunohistochemical technique was used to determine the expression of BSP and OPN in dental tissues and the surrounding bone at different time points.

RESULTSImmunoreactivitis of BSP and OPN were present in matured ameloblasts, dentinoblasts, cementoblasts, osteoblasts, and the matrix. The expression of BSP and OPN in cementum and alveolar bone was stronger than that in enamel and dentine. In cementum and alveolar bone, BSP appeared to be concentrated in unmineralized and mineralized tissues, but OPN was concentrated in the mineralizing frontier and reversal line.

CONCLUSIONSBSP and OPN play an important role in the development and mineralization of rat mineralized tissues. The expression of BSP was different from OPN, indicating their different functions.

Animals ; Immunohistochemistry ; Integrin-Binding Sialoprotein ; Male ; Osteopontin ; Rats ; Rats, Sprague-Dawley ; Sialoglycoproteins ; analysis ; Tooth ; chemistry

Objective:To translate the health literate healthcare organization 10 item questionnaire(HLHO-10) into Chinese and examine its reliability and validity.Methods:The Chinese version of HLHO-10 questionnaire(HLHO-10-C) was developed by following the Brislin translation model of translation, back translation, cultural adaptation and questionnaire epistemological survey.Five experts and 1 071 medical staff from 24 healthcare organizations in Zhejiang province were selected to conduct the validity and reliability test of the HLHO-10-C.Results:The content validity indices at the item level and total questionnaire level of HLHO-10-C were from 0.8 to 1.0 and 0.96 respectively, and the results of the exploratory factor analysis showed good structural validity.Conclusions:HLHO-10-C proves adequate reliability and validity to serve as a tool for healthcare organizations in evaluating and becoming HLHO. It can also help the implementation of the Healthy China Initiative(2019—2030), which is a performance assessment mechanism for health education and promotion of healthcare providers and health care organizations.