Objective To investigate the effects of lead on the vitality, differentiation, special functional markers and morphological changes of the osteoblasts in newborn rats, which helps to clarify the toxicity mechanism of lead on the skeleton development in children. Methods The osteoblasts were isolated from the calvariae in newborn Wistar rats and cultured in DMEM medium. Lead acetate at different concentration (0, 0.1, 0.5, 1, 5, 10, 50, 100 ?mol/L) was added for 24 h, 48 h, 72 h. The vitality of the osteoblasts was measured by MTT methods; The activity of alkaline phosphatase(ALP) was measured by pNPP method. The protein content of the osteoblasts was determined by Coomassie brilliant blue method. The levels of osteocalcin in both secreted (in medium) and cellular of osteoblasts were determined by radiate immunochemistry methods. The optical structure was determined by LM. Results The vitality decreased significantly when the dose was at more than 50 ?mol/L, especially after 72 h. The activity of ALP decreased significantly when more than 0.5 ?mol/L, whereas the levels of secreted osteocalcin decreased significantly when at 0.1 ?mol/L. It appeared lead had some toxicity on the shape and number on the osteoblasts. Conclusion Lead acetate has adverse effect, at higher doses, on the vitality, protein content, ALP, osteocalcin and optical structure, maybe it is one of the mechanisms of lead influencing the development of the osteoblasts even the skeleton.

We compared the results of heating the joint and of applying the Hot-spike and electric stimulus to the YUSEN and SANINKO points to disperse the fluid at the Knee.
The tests with the points resulted in little change in the fluid level at the Knee.
This time we measured the circumference of the Knee. A therapist established the standard measuring method, and tried to make the error decrease.
While they were observing the circumference of the Knee, they would not allow anyother medical treatments or any movement by the patient.

Recently, cosmetic acupuncture has converged on the acupuncture industry currently, cosmetic acupuncture is in strong demand in clinical scenes every day, and it is a part of classes at training institutions or universities of acupuncture.
Only ten years ago, it was taboo to use acupuncture cosmetically except for special occasions, and acupuncturists and moxa-cauterizers did not have much interest in it. However, against the background of its being required for the "beauty"field, it is seen off and for the wish for youth and beauty of us hoping for a higher quality of life. In June 2010, the 59th annual meeting of Japan Society of Acupuncture and Moxibustion held the International Conference Centre in Osaka Nakanoshima. This acupuncture congress came up with a great topic, and the extension of practical skills for cosmetic acupuncture took place for the first time. We got a high response at the congress. The hall capacity was 350 people and was full. People were even standing in the back because there were no seats left. Therefore, we would like to request you, professors teaching people exhibiting some practical skills at the congress, to write about your thoughts for cosmetic acupuncture and the practice of it, which we hope will be future prospects for cosmetic acupuncture.

From September of 2006,Department of Pathophysiology opened laboratory to the undergraduates of 2004 grade.Stu- dents chose titles and designed experiments by themselves,and after expert teachers' assessment,they put them into practice. Functional Laboratory of the college is in charge of the oprtation and administration of it.Through this semester's practice and exploration,we got satisfactory effect and great welcome from students.

Objective To explore the expression of DC?SIGN, the phenotype of dendritic cells (DCs), on podocytes, and its role in immune and inflammatory responses of lupus nephritis (LN). Methods DC?SIGN and IgG1 expression in renal tissues of lupus nephritis patients were observed by immunohistochemistry and immunofluorescence. The 4?week old LN mice were randomly divided into the experimental group and the intervention group. C57BL/6J mice were used as normal control group. Mice of the intervention group were injected anti?DC?SIGN antibody at 6?week old. Mice were sacrificed at 16, 20, 24, 28?week old respectively, to observe the mice renal function and pathological changes. And DC?SIGN and IgG1 expression in renal tissue were observed by immunohistochemistry and immunofluorescence. In addition, mice podocytes were treated with serum of LN mice. Flow cytometry was used to investigate the expression of MHC II, CD80 and DC?SIGN expression on podocytes. Mixed lymphocyte reaction was used to detect the ability of stimulating T cells proliferation. IFN?gamma and IL?4 in supernatant were determined by ELISA. Results (1) Expression of DC?SIGN and IgG1 was found in glomeruli of lupus nephritis patients. (2) Accompanied by increased proteinuria of LN mice from 20?week old (P<0.01), DC?SIGN and IgG1 expression was found in glomeruli, and the renal function deteriorated up to 24 week?old (P<0.01). Mice with anti?DC?SIGN antibody intervention appeared reduced proteinuria and remission of renal function (P<0.01). (3) After stimulated by serum of LN mice, the expression of DC?SIGN, MHC II and CD80 was up?regulated, stimulation of T cell proliferation was enhanced (P<0.01), and IFN?gamma/IL?4 ratio increased (P<0.01). Anti?DC?SIGN antibody treatment down?regulated the expressions of DC?SIGN, MHC II and CD80 on podocytes, decreased the ability of stimulating T cell proliferation and lowered the ratio of IFN?gamma/IL?4 (P<0.01). Conclusions Podocytes in lupus nephritis can play DC?like function through the expression of DC?SIGN, which may be involved in immune and inflammatory responses of renal tissue. However, inhibiton of DC?SIGN can depress immune function of podocytes and have prevention and treatment effect.

Objective To optimize the best subculture cycle through studying the influence of subculture time on tube plantlet growth of Dendrobium huoshanense and the change of medium composition.Methods Height,tiller,fresh weight,and chlorophyll content of the tube plantlet and pH value,water content,and sugar content of the medium were measured after different cycles of the subculture,the cost of culture medium for subculture was calculated as well.Results The height of the tube plantlet increase 282.86%,the tiller increase by 3.5 times,fresh weight reaches its maximum,chlorophyll content of the tube plantlet almost reaches its maximum after 40 d subculture;while water content and sugar content of the medium are decreased to the lowest point,pH value of medium(

At present, the development of hospital in transition period the doctor-patient contradiction, mutu-al trust foundation weak such outstanding contradictions and problems, based on the analysis of facing the outpatient service charge first overall service consciousness; Contradiction link concentration; Hospital information system support does not reach the designated position; Charge personnel are not familiar with services such as business management status, on the basis of train first service consciousness concrete measures are put forward. Namely:strengthen the psychological counseling, advocate humanistic care;carry out training assessment, improve human-istic quality;strengthen the construction of standardization, the standard outpatient service charging process;develop information platform, optimize charging way;strengthen the department cooperation, build a system integration work.

Objective To study the hemostnsis effects of compression cords oppressing after femoral artery puncture and encheiresis. Methods Self-made compression cords were used to stop bleeding of femoral artery puncture sites in 2164 patients underwent 3046 cases of arteriopuncture encheiresis. The total eases were divided into group A (1482 eases) and group B (1564 cases) according to different treatment of oppression and breaking duration which were 8-10 hours for group A and 5-6 hours for group B, the hemostasis effects, discomfortableness and related complications within two groups were evaluated. Results The proportion of patients who got good hemostasis effects were 99.2% in group A, 99.0% in group B. There was no difference in hemostasis effects between two groups (P> 0.05). While the rate of discomfortableness in group A (63.8%) was higher than that in group B(42.9%) (P<0.05). The related serious complications were 2 patients in group A, but no occurred in group B. Conclusion Self-made compression cords op- pressing and 5-6 hours breaking time course can achieve both finer hemostasis effect and lower frequency of complications after femoral artery puncture and encheiresis.

Identifying the importance of improving pricing control in military hospitals based on a review of the status quo of such management.In addition to an analysis of difficulties in pricing control in these hospitals,proposing to build an efficient pricing control system with a better understanding of the significance of pricing control.Establishing rules and regulations on medical charges to regulate pricing;making use of computer networks in the management for medical charges transparency;enhancing charges securitization to rule out excessive charges;improving general competence of pricing workers to regulate pricing control,All these five measures will help enhance pricing control in military hospitals.