The pathogenesis of barium-induced severe myocardial necrosis in dietary potassum-depleted rats was investigated. In this study four experiments were performed. The results of these experiments suggest that there exist a complex pathogenesis of myocardial lesion produced by barium in condition of potassium deficiency. The main mechanism seems to be the direct stimulating effect of barium on myocardial contractibility. It seems unlikely that barium-induced constriction of coronary arteries, hypertension, hypokalemia, and increased catecholamine secretion are the basic mechanism of the myocardial necrosis. But these factors may play a promoting or aggravating role in the development of the myocardial lesion. Potassium deficiency not only acts as a conditioning factor for barium-induced myocardial necrosis, but also relatively increases the cardiotoxity of barium. This may be the principal pathogenesis of severe myocardial necrosis produced by barium in combination with potassium deficiency.

Objective:To investigate the relationship between poly(ADP-ribose)polymerase(PARP) inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro.Methods:The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro.MTT assay was used to determine the growth activity of CT26 cells.The expressions of PARP and NF-?B p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope.Results:The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52%、27.63% and 39.93%,respectively.The inhibitory rate increased with the raising of the 5-AIQ concentration(0.1mmol/L,0.5mmol/L,and1.0mmol/L),and the difference was significant(P

The effects of nitrendipine (Nit) on myocardial contractility, myocardial succinate de-hydrogenase (SDH) and cytochrome oxidase (CCO) activities in streptozotocin (65mg/kg) diabetic rats were studied. Four weeks after the induction of diabetes, the rats were treated with Nit (30mg ?kg -2/day) for 4 weeks. The results showed that ventricular diastolic function was affected after 4 weeks of diabetes, and both ventricular diastolic and systolic functions were obviously involved after 8 weeks. These changes were significantly improved in diabetic rats receiving Nit treatment. The myocardial SDH and CCO activities in diabetic animals were markedly lower as compared to controls. The attenuation of these enzyme activities in diabetic rats was significantly reversed by administration of Nit. These findings suggest that Nit treatment may exhibit some beneficial effects on diabetic cardiomyopathy.

ObjectiveTo investigate the effect of down-regulating epidermal growth factor receptor (EGFR) expression on Bleomycin induced pulmonary fibrosis in mice.MethodsForty 4 ～6 week aged C57BL/c male mice were randomly divided into control,bleomycin,bleomycin plus EGFR RNAi groups and RNAi negative control group.Bleomycin group were treated with bleomycin (3 mg/kg) by endotracheal injection on day 0,control group were treated with PBS.And bleomycin plus EGFR RNAi group were received EGRF siRNA plus bleomycin intratracheal administration.RNAi negative control group received negative EGRF siRNA plus bleomycin intratracheal administration.Mice were sacrificed 10 days after the treatments.Hydroxyproline (HYP) assay was performed in the lung tissue.The lung tissue slides were examined pathologically with H.E.staining,and EGFR mRNA expression was detected by reverse transcriptasepolymerase chain reaction (RT-PCR) technique.Western blot were performed to identify the protein level.of total EGFR and phosphorylated EGFR.ResultsHistological examination of lung specimens demonstrated that EGFR siRNA administration lessened lung fibrosis induced by bleomycin and significantly reduced HYP content (543.00±25.89 vs 900.73±31.77,P＜0.01).EGFR mRNA (0.31±0.05 vs 0.75±0.08,P ＜0.01) and protein expression(1.53±0.47 vs 2.56±0.37,P ＜0.01) in EGFR siRNA-treated mice was significantly decreased.The expression of phosphorylation of EGFR protein (1.78±0.35 vs 2.84±0.51,P ＜0.01) and EGFR protein in RNAi group was less than in bleomycin group.Conclusions EGFR RNAi reduced the BLM-induced lung fibrosis by inhabiting EGFR activation.

Humans ; Maintenance Chemotherapy ; Multiple Myeloma ; therapy

Oxidative stress plays an important role in the progression of diabetes mellitus. As a potent antioxidant, alpha-lipoic acid is able to clear free radicals and alleviate oxidative damages and therefore has been widely applied in the clinical management of diabetes mellitus. This article summarizes the clinical application of alpha-lipoic acid in alleviating diabetes mellitus-related oxidative damages, protecting vascular lesions, treating diabetic polyneuropathies, and modulating insulin sensitivity.

Parenteral nutrition associated cholestasis (PNAC) mainly occurs in children. Currently identified risk factors include premature infants, lack of enteral feeding, repeated infections, and toxicity or nutrient deficiency of parenteral nutrition solution. Recent studies have shown that nuclear receptor-mediated bile acid transporter may be the key site of PNAC pathogenesis, in which some cytokines play important roles. New anti-cholestatic therapy based on the regulation of expression of these molecules may prevent end-stage liver disease caused by PNAC.

Objective To investigate the treatment effect and nursing of intravitreous injection of Avastin combined with laser photocoagulation in macular edema caused by vein occlusion.Methods 38 patients(38 macular edema eyes)who had macular edema caused by retinal vein occlusion and accepted intravitreous injection of Avastin combined with laser photocoagulation were selected.Relevant nursing was given before and after the clinical treatment.The visual acuity and retinal thickness were observed and recorded.Results Visual acuity was improved in 36 eyes(94.7％),no change occurred in 2 eyes(5.3％).The thickness of macular was statistically significant compared with before treatment and 2,4,6 weeks after treatment.Conclusions Intravitreous injection of avastin combined with laser photocoagulation is effective on macular edema caused by vein occlusion.Successful treatment is guaranteed by psychological nursing,treatment guidance and health guidance.

Autophagy and apoptosis are two kinds of important ways of neuronal death in cerebral ischemia.The interaction of both in ischemic penumbra may alleviate or aggravate cerebral ischemic injury.This article reviews the role of autophagy and apoptosis in cerebral ischemia and their potential regulating mechanisms of interaction.

Synergistic Innovation is the developing trend and requirements of the times and society.Southern Medical University practiced joint mode among colleges and universities for excellent medical talent cultivation.Based on preponderant discipline and taking mechanism innovation as the key points,Southern Medical University actively explored the diversification mode of synergistic innovation.