Objective To investigate the relationship of thyroid hormones and serum lipid profile during Pregnancy. Methods 30 cases of healthy pregnant women were recruited in the study. The thyrotropin (TSH), free triiodothyronine (FT3), free thyroxine (FT4) and serum lipid profile were examined at 9 ~ 12, 14 ~ 17, 23 ~ 26 and 37 ~ 40 weeks of gestation and the correlations between them were analyzed. Results Positive correlation could be found between serum TSH and total cholesterol (CHOL), triglyceride (TRIG), low density lipoprotein cholesterol (LDL-C), ApolipoproteinA-I (APOA-I), ApolipoproteinB (APOB). Negative correlations could be found between serum FT3, FT4 and total cholesterol (CHOL), triglyceride (TRIG), low density lipoprotein cholesterol (LDL-C), ApolipoproteinA-I (APOA-I), ApolipoproteinB (APOB). And no correlation was found between serum thyroid hormones and high density lipoprotein cholesterol (HDL-C). Conclusion The thyroid hormones were closely related to serum lipid profile except of HDL-C.

Objective To explore the efficacy and safety of different doses of budesonide mixed suspension atomization inhalation in children with post infectious cough,and provide reference for the rational use of budesonide.Methods One hundred and twenty children with post infectious cough aged 2-14 years old were selected and divided into three groups by random digits table method (40 cases in each group).Control group was treated with 4 mg once montelukast sodium chewable tablets for 2-5 years old children,or 5 mg once for 6-14 years old children.Low-dose group was treated 2 times/d with a dose of 0.5 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group and high-dose group was treated 2 times/d with a dose of 1.0 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group.The outcome of curative effects were recorded and analyzed after 7 days of treatment.Results The total efficacy rate was 70.0％(28/40),52.5％(21/40),35.0％(14/40) in high-dose group,low-dose group and control group after 3 days of treatment,and there was significant difference between high-dose group and control group (P＜ 0.01).The total efficacy rate was 92.5％(37/40),90.0％(36/40),72.5％ (29/40) in high-dose group,low-dose group and control group after 7 days of treatment,and there was no significant difference(P ＞ 0.025).Few adverse events associated with inhaled corticosteroids,such as thrush,sore throat,hoarseness were observed in three groups.Conclusions Budesonide mixed suspension atomization inhalation alleviates symptom of post infectious cough significantly with no obvious side effects,even in high-dose group which has a more outstanding efficacy.Therefore,this therapy is valuable in clinical application.

Objective To evaluate the diagnostic accuracy of 4 μg/mL vancomycin screening plate (4VA)for vancomycin-non-susceptible Staphylococcus aureus.Methods The diagnosis trials on 4VA for vancomycin-non-susceptible Staphylococcus aureus were searched in the databases such as PubMed(1 966 to Aug.2013),The Cochrane Library(the second phase of 2013),ISI Web of Knowledge(1 980 to Aug.2013),CNKI (1 964 to Aug.2013),VIP (1 989 to Aug.2013)and Wanfang(1 998 to Aug.2013),mean-while the manual.Two reviewers evaluated the quality of the included trials according to the quality assessment of diagnostic accu-racy studies,and then meta-analysis was performed using Meta-Disc 1.4 software.Results A total of 3 trials involving 974 partici-pants were included.The results of meta-analysis showed that the weighted sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and diagnostic odds ratio,were 0.805 [95%CI (0.047 to 0.563)],0.921 [95%CI (0.901 to 0.938)],10.804 [95%CI (5.5 1 1 to 21.181)],0.1 62 [95%CI (0.71 to 1.0)]and 69.721 [95%CI (1 1.740 to 414.06)]respectively.Conclusion 4VA for vancomycin-non-susceptible Staphylococcus aureus has a very high specificity and sensitivity,so 4VA could be used to creening plate (4VA)for vancomycin-non-susceptible Staphylococcus aureus in clinic.

Objective To compare the therapeutic effect in two kinds of inhalation in infant asthmatic bronchitis.Methods Two hundred and twelve asthmatic bronchitis children were randomly divided into group A (103 cases) and group B (109 cases).The children in two groups were treated with conventional therapy,including antibiotics,fluids and other treatment.The children in group A were given budesonide 1 mg,terbutaline 5 mg plus 0.9％ sodium chlorine in 2 ml,with oxygen driven inhalation therapy,2-4 times/d.The children in group B were given albuterol sulfate inhalation aerosol 1-4 spray/times,4-6 times/d,inhaled fluticasone quantitative aerosol spray 1-2 spray/times,2 times/d,15 s/times.The course of two groups was 5-7 d.The therapeutic effect was compared.Results The total effective rate in group A and group B were 92.2 ％ (95/103) and 97.2 ％ (106/109),there was no significant difference (x2 =2.73,P ＞ 0.05).The relieve time of cough,gasping and wheezing,hospital stay in group B were shorter than those in group A [(12.00 ± 2.75) h vs.(29.00 ± 1.25) h,(10.00 ± 1.45) h vs.(31.00 ± 2.75) h,(13.00 ± 1.55) h vs.(33.00 ± 2.33) h,(7.20 ± 0.65) d vs.(8.70 ± 1.35) d],the cost of hospitalization in group B was lower than that in group A [(2.00 ± 0.30) thousand Yuan vs.(2.50 ± 0.55) thousand Yuan],there were significant differences(P ＜ 0.05).Conclusions The albuterol inhalation solution combined with fluticasone has the advantages of rapid efficacy,short course,low cost,easy to accept and adjust.It is worth to spread in primary hospital.

BACKGROUND:Overtraining is a series of functional disorder or pathological state induced by continuous fatigue accumulation because exercise load and body function are incommensurate to each other. At present, commonly used methods for establishing rat models of overtraining included treadmil , swimming and climbing rod, but treadmil is comparatively accepted in the world.
OBJECTIVE:To establish the standard of overtraining rat model and to implement objective of model establishment by dynamical y monitoring biochemical indexes and observing behavioral changes.
METHODS:A total of 16 Sprague-Dawley rats were randomly assigned to model and blank control groups. The model group received movement training according to the plan. After adaptable feeding, training was performed, 6 days every week, with a rest of 1 day. Increasing intensity on treadmil was used. From the first week of training, the speed, gradient and running time were gradual y increased. However, the blank control group was conventional y fed, without any training.
RESULTS AND CONCLUSION:Behavior changes of the training rats were arisen after five weeks. Serum creatine kinase levels increased continuously in training process, and higher than basic levels at 5 weeks (P<0.01). Serum urea nitrogen levels persistently increased, and higher than basic levels at 3 weeks (P<0.05). Hemoglobin and serum testosterone levels increased and then decreased, and significantly lower than basic levels at 8 weeks (P<0.05). Behavioral y, overtraining appeared. Simultaneously, hemoglobin and serum testosterone levels were significantly lower than basic levels. Serum creatine kinase and serum urea nitrogen levels were significantly higher than basic levels. These results indicated that the body was in overtraining state. The standard of overtraining rat model was established in this study. The overtraining rat model was established according to the training program when the training was lasted for 8 weeks, the training speed was 30 m/min;every training time was 110 minutes, and the gradient was 15°.

[Objective]To observe the effect of Chinese herbs for activating blood stasis on the shape and function of the endothelial cells in rat aorta.[Method]Rat models of PCOS have been established according to Poresky,randomized into animal model and Chinese medicine group(TCM group),normal rats were control group.Measure the serum level of FSH,LH,T,INS and plasma ET PAI-1,and observe the endothelial cell morph.[Results] In the model group,the endothelia cells of the aorta were contracted,with discontinuous basement membrane,inflated chondriosome,the middle layer smooth muscles tending to move inside and out of the normal shape of intraendothelial junction.In the TCM group,the endothelial cells appeared flat,arranging along the long axis of the blood vessels,normal endothelial cell integrity with scanty cytoplasm and the microscopic appearance shows highly developed golgiosome rough endoplasmic reticulum and smooth endoplasmic reticulum.The model group had a more increased serum level of PAI-I and ET than those of the control group,and showed the significant difference(P0.05).The TCM group had more decreased level of ET than those of the normal group(P

Toll-like-receptors(TLRs) have been found to play a critical role in immune response of hosts.They can trigger innate immune responses by detecting pathogen-associated molecular patterns(PAMPs).This paper reviews the roles of several toll-like receptors(TLR4,TLR5,and TLR9) in radioprotection and the related mechanisms,so as to provide theoretical evidences for further studying their roles in radioprotection.

Purpose:Expression of MEK-1 was studied by regulating ?V integrin and E-cadherin on MCF-7 breast cancer cells.Methods:Using cell transfected techniques site-mutated genes were inserted into MCF-7 cells and made MCF-7 cells containing MEK-1-GFP,Ala 222 MEK-1-GFP,Asp 222 MEK-1-GFP ,respectively. These cells were tested with immunoblot analysis and immunofluoresence technique to determined the change of MEK-1 expression and location of MEK-1 in cells.Results:Deactivated Ala 222 MEK-1-GFP inhibited the activity of MAPK,active Asp 222 MEK-1-GFP increased the activity of MAPK. Ala 222 MEK-1-GFP increased the expression of E-cadherin,did not increase the expression of ?V integrin and inhibited FAK phosphorylation. MEK-1-GFP and Asp 222 MEK-1-GFP did not affect the expression of E-cadherin ,but upregulated the expression of ?V integrin,increased FAK phosphorylation.Conclusions:MEK-1 regulated the expression of ?V integrin and E-cadherin,FAK is the key factor that induced signal pathway of ?V integrin. It is possible that MEK-1 regulated FAK phosphorglation by regulating expression of ?V integrin and E-cadherin.

AIM: To establish a method for determing GC fingerprint of the chemical constituents in Bulbus lilii. METHODS: GC/MS were used to optimize the fingerprint determination, and to identify the main peaks in the GC fingerprint. RESULTS: A preferable determination for GC fingerprint of the chemical constituents in Bulbus lilii.was established. CONCLUSION: A general acquaintance of the chemical constituents in Bulbus lilii.can be obtained by using the preferable GC fingerprint determination method,which is useful for quality evaluation of the crud drug of Bulbus lilii.

Objective Studies have shown that estrogen plays profound roles on nerve system,but the mechanism is now poorly understood.In order to address this question,the distribution of the newly cloned estrogen receptor ?(ER\|?) immunoreactivity(ER\|?\|ir) was examined in the brain of adult female rats. Methods Using nickel ammonium sulfate intensified immunohistochemistry technique. Results 1.ER\|?\|ir was mainly localized in the nuclei of neurons,it was also found in the perikaryon and neuritis;while in very few neurons,positive signals were only detected in the perikaryon and neuritis;2.High level of ER\|?\|ir positive signals were detected in the anterior olfactory nucleus,cerebral cortex,Purkinje cells,vertical limb of the diagonal band,superior vestibular nucleus,endopiriform nucleus,lateral amygdaloid nucleus,red nucleus and loci ceruleus;moderate level of ER\|?\|ir was detected in the medial septal nucleus,posterior cortical nucleus of the amygdaloid,CA3 and CA4 of the hippocampus,dentate gyrus,bed nucleus of the stria terminalis,supraoptic nucleus;weak signals were detected in the nuclei of hypothalamus,oculomotor nucleus,claustrum and some nuclei of the amygdaloid complex.Conclusion\ ER\|? immunoreactive neurons are extensively distributed in the brain of the adult female rats;this receptor may be involved in different brain function in different brain nuclei. \;[