Newborn larva (NBL) antigens of Trichinella spiralis were analysed by Immuroblot,and were comparied with the adult and muscle larva antigens.The SDS-PAGE patterns of NBL somatic constituents consisted of about 40 polypeptide bands,which were obviously different from those of adult and muscle larva.Immunoblot analysis indicated that immunization with NBL could induce a stage specific immune response.The molecular weight of specific NBL antigens were 129,120,89,87,79,74,72,64,58,43,40,38,34,32,and 20kDa.But during the natural course of the infection,we could not detect the antibodies of anti-NBL in the host.

Atrial Fibrillation ; pathology ; Humans ; Peptidyl-Dipeptidase A

Objective:To investgate the ease effects of local injection of ursolic acid both on orthodontic tooth movement distance and tooth root resorption in the rats, and to clarify its mechanism.Methods:Ninety-six male Wistar rats were used to establish models of orthodontic tooth movement.After the successful establishment of models,all the model rats were randomly divided into 0 (control group),0.5,1.0 and 2.0 mmol·L-1 ursolic acid groups (n=24).The rats were locally injected with different doses of ursolic acid into the palatal submucosal area adjacent to the right upper first molar ,once per 3 d,each time 50μL.The rats were respectively sacrificed on the days 1,3,5,7,10,14,21 and 28,then the distances of tooth movement were measured.HE staining was used to observe the morphologic changes of the root tissue of rats.Results:The tooth movement distances of rats in control,0.5,1.0,and 2.0 mmol· L-1 ursolic acid groups were increased with the prolongation of time (P<0.01).Compared with control group,the tooth movement distances of rats in 0.5 mmol·L-1 ursolic acid group 3,7,14,21 and 28 d after forcing had statistical significance (P<0.05 or P<0.01 );the tooth movement distances of rats in 1.0 and 2.0 mmol· L-1 ursolic acid groups 1 d after treatment had no statistical significance (P>0.05),but at the other time points,the tooth movement distances of rats had statistical significance (P<0.01).The tooth movement distances of rats had statistical significance between 0.5,1.0 and 2.0 mmol·L-1 ursolic acid groups 5,7,10,14,21 and 28 d after forcing (P<0.05 or P<0.01).The morphological results showed that the resorption pits were found on the surface of tooth root with the prolongation of loading time;in addition,with the increasing of the doses of ursolic acid, the absorption of root surface was relieved. Conclusion:Local injection of ursolic acid at the doses of 0.5,1.0 and 2.0 mmol·L-1 can reduce the distance of orthodontic tooth movement in the rats, and the distance of tooth movement in the rats is reduced with the increasing of its doses,and ursolic acid has the ease effect on the orthodoutic tooth root resorption.

Objective To quantitatively estimate upper motor neuron (UMN) lesion with the triple stimulation technique (TST) in amyotrophic lateral sclerosis (ALS). Methods Fifty ALS patients and 22 normal controls were enrolled in the study. Patients were examined clinically with conventional transcranial magnetic stimulation (TMS) and TST at abductor digiti minimi. Central motor conduction time (CMCT),motor evoked potential ( MEP), resting motor threshold ( RMT), compound muscle action potential ( CMAP), modified ashworth scale ( MAS), Medical Research Council scale and modified ALS scale were assessed and their correlation to disease progress was analyzed. Results The TST amplitude ratio was significantly decreased in ALS patients with UMN signs(28 cases 62. 0% (40. 7%, 75.9% ) ), compared with controls ( 96. 9% ± 2. 6% ) and ALS patients without UMN signs ( 22 cases 95.6% ( 85.4%,100. 0% ) ;Z = -4. 827, -5.435, both P =0. 000). The abnormal rates of the TST amplitude in ALS with UMN signs, ALS without UMN signs and controls were 89. 3%, 27.3% ,9. 1% respectively. The abnormal rates of the TST amplitude, the latency of MEP, CMCT, RMT in detecting UMN lesions were 89. 3%,64. 3%, 53.6%, 64. 3% , 78. 6%, respectively. The TST amplitude was significantly correlated to tendon reflex in right arm ( r = 0. 690, P = 0. 000), with modified ashworth scale ( MAS, r = - 0. 772,P = 0. 000),with diagnostic degree ( r = 0. 483, P = 0. 000), with RMT ( r = - 0. 774,P = 0. 000), the latency of MEP (r = - 0. 444, P = 0. 005 ), motor evoked potential/compound muscle action potential of erb' s ( MEP/ CMAPerb, r = 0. 685, P = 0. 000 ), MEP/CMAPerb in facilitation ( r = 0. 770, P = 0. 000). Conclusions TST appears to be a more accurate and sensitive measure of detecting and quantifying UMN abnormality in ALS patients than the other parameters. TST may reveal the subclinical UMN impairment in ALS and provide an accurate diagnosis assessment for UMN loss in ALS and an objective scale for monitoring the progression of disease.

The time of six meridians diseases tending to be cured in Shang Han Lun (Treatise on Cold Diseases) is always one of the key points for study, but up till now no satisfied explanations are made. The authors try to study it on the basis of the theory of "three-yin and three-yang" according to the relationship between human body and the environment.

Objective To explore the effect of fetal thymus transplantation on enhancing the immunity of patients with late-stage malignant tumors. Methods The whole thymus of 24～32 weeks fetus was transplanted into the forearm of the patients with late-stage milignant tumors by microvascular surgery. The levels of serum IgA,IgG and IgM were measured before and at the third, 6th and 12th months after operation. No immunosuppressive regimen was performed in all the patients after operation. The follow-up period was 1 to 2 years. Results The clinical symptoms of all the patients improved after treatment, and no rejection reaction occurred. The levels of serum IgA,IgG and IgM significantly increased 3,6 and 12 months after treatment(t≥4.23,P

Central Nervous System Viral Diseases ; complications ; virology ; Child ; Epstein-Barr Virus Infections ; complications ; virology ; Female ; Follow-Up Studies ; Herpesvirus 4, Human ; Humans ; Lymphocytosis ; etiology ; virology ; T-Lymphocytes

OBJECTIVETo explore the mechanism of natural moxibustion on regulating immune imbalance in asthma rats.

METHODSSeventy SD male rats were divided into a normal group, a placebo group, a dexamethasone group, a big-cake for long-course moxibustion group, a big-cake for short-course moxibustion group, a small-cake for long-course moxibustion group and a small-cake for short-course moxibustion group, ten rats in each one. The rat model of asthma was established by egg albumen sensitization and stimulation in all the groups except the normal group. The natural moxibustion was used in all moxibustion groups, in which big cake of 1 cmX 1 cm size was used in the big-cake groups and small cake of 0.5 cmX 0. 5 cm size was used in the small cake groups. According to relevant acupoints, the natural moxibustion was performed, 5 h per time, once a day. Four times of treatment was considered one course, and three courses were required in the long-course groups and one course was required in the short-course groups. Intraperitoneal injection of dexamethasone was applied in the dexamethasone group, which had the same course as long-course moxibustion group. After the treatment, changes of EOS in peripheral blood of asthma rats were observed; enzyme linked immunosorbent assay (ELISA) was applied to test the contents of IgE, IL-4 and IFN-gamma in the lung tissue; real-time Q-PCR method was adopted to measure the expression level of transcription factor T-bet and GATA binding protein 3 (GATA-3) in the lung tissue.

RESULTSCompared with the normal group, the EOS in whole blood as well as IL-4 and IgE in plasma were all increased in the placebo group (all P< 0. 01), IFN-gamma in plasma was obviously decreased (P<0. 01); while the levels of EOS, IgE and IL-4 were significantly reduced (all P<0. 01), the content of IFN-gamma was increased (P<0. 01) in all moxibustion groups and dexametnasone group. Compared with the normal group, the expression of T-bet mRNA in the placebo group was significantly reduced (P<0. 01). Each treatment group could significantly increase the expression of T-bet mRNA and reduce that of GATA-3 mRNA (P<0. 01). Compared with the short-course moxibustion groups, the expression of T-bet mRNA was obviously increased in the long-course moxibustion group and dexamethasone group (both P<0.01), and that of GATA-3 mRNA was reduced (P<0.01). There was no significant difference between long-course moxibustion group and dexamethasone group (P> 0. 05), and also no significant difference could be seen between big-cake moxibustion group and small-cake moxibustion group (P>0. 05).

CONCLUSIONThe natural moxibustion could obviously reduce airway inflammation in asthma rats. With time passing, the efficacy is enhanced, indicating evident timeliness, which has no apparent relationship with the size of moxibustion cake.

Animals ; Asthma ; genetics ; metabolism ; therapy ; Cytokines ; genetics ; metabolism ; Humans ; Male ; Moxibustion ; Rats ; Rats, Sprague-Dawley ; Transcription Factors ; genetics ; metabolism

Objective To evaluate the clinical significance of measuring IL-13 in serum and dynamics of pulmonary function in infants with bronchiolitis.Methods A total of 50 infants with bronchiolitis(30 in acute phase,20 in recovery phase) and 20 infants in healthy controls were recruited into this study.IL-13 level in serum were detected with Sandwich enzyme linked immunosorbent assay(ELISA),at the same time the object′s respiratory function were tested,then the above indexes were compared.Results The level of serum IL-13 in recovery phase of bronchiolitis was significantly lower than that in acute phase of bronchiolitis and those of both 2 groups were significantly higher than that in control group(all P0.05).Conclusions IL-13 plays a role in pathogenesis in infants with bronchiolitis.The reduction of level of IL-13 will contribute to recovery of infants with bronchiolitis.During the acute phase of bronchiolitis higher resistance can be seen.