Advanced colorectal cancer mainly classified as locally advanced,locally recurrent and distally metastatic colorectal cancer.Distal metastasis of colorectal cancer includes peritoneal metastasis,liver,lung and other organ metastasis.Surgical resection remains the only possible cure for patients with advanced colorectal cancer.Preoperative systemic evaluation based on radiographic images,especially the evaluation on the resectability of hepatic metastatic lesions from colorectal cancer is very important.Neo-adjuvant and adjuvant therapy increased the resection rate of primary and metastatic lesions of advanced colorectal cancer.In recent 10 years,with the development of oncolegical surgery,treatment modality of multi-disciplinary team has been proposed and implemented,which increased the survival time and improved the life quality of patients with colorectal cancer.

BACKGROUND:Continuous monitoring is required in the survival, distribution, migration, proliferation and differentiation of transplanted stem cel s in vivo thereby to assess its efficacy and safety.
OBJECTIVE:To analyze the characteristics of literature addressing stem cel markers and MRI tracer in the past 10 years.
METHODS:The first author retrieved CNKI and Web of Science by computer for articles about MRI tracing of stem cel s published from January 2005 to December 2014, using the keywords of“stem cel s, magnetic resonance imaging, tracing technique”that appeared in the title and abstract. Then, the retrieved articles were further analyzed.
RESULTS AND CONCLUSION:Currently, tracing technique has been widely used in stem cel therapy, organ transplantation, bacterial infection, gene expression and new drug development. Stem cel tracing technologies include isotope tracing, antigenic labeling, fluorescence protein labeling, fluorescent dye labeling, nuclear magnetic resonance contrast enhancer labeling, Lac-Z gene labeling, Y chromosome labeling. Recently, with the development of molecular imaging, MRI tracing techniques have been increasingly used to assess the efficacy and safety of stem cel transplantation.

Objective Analysis the features of extracolonic cancer of HNPCC.Method 32 families with HNPCC were registered and followed up.Results Among 12 families with typical HNPCC,8 cases in 6 families and 1 case of nontypical HNPCC families developed extracolonic cancer. Endometrial and gastric cancers are the most common cancers.Conclusions The extracolonic cancers are the important part in the cancer spectum of HNPCC. Among Chinese families, endometrial and gastric cancers are more common.

The expression of ZNF191 mRNA in 31 ovarian malignant tumors and 31 normal ovary tissues were investigated by reverse transcription-polymerase chain reaction (RT-PCR). It was found that expression level of ZNF191 mRNA in malignant ovarian tumors was much less than that in normal ovary tissues (P

Objective To investigate the change in heat shock protein 70(HSP7O) in the brain tissue of fetal and newborn rats after prenatal hypoxic adaptation and the possible mechanism of the protective effect. Methods Twenty-two 22d pregnant Wistar rats were randomly divided into two groups: GroupⅠ(hypoxic adaptation group) and group Ⅱ(control group). The animals in group Ⅰ were placed in a tightly closed hypoxic adaptation chamber, of which the oxygen and carbon dioxide concentrations were monitored. The pregnant rats were taken out and exposed to fresh air for 5 mm when the 02 % in the chamber was reduced to 15 %, then the pregnant rats were placed back in the chamber and the above process was repeated once. The animals were then left for their natural labor. In control group the pregnant rats underwent the same process but the chamber was not tightly closed(O2 %= 21 %). Prenatal rats were delivered by cesarean section at lb. 3h, 8h, 24h, 48h, 72h, 120h and 168h after hypoxic adaptation and decapitated and brain was removed. Seven newborn rats from each group were decapitated and brain was removed for determination of HSP70 expression with immunohistochimical technique. Results No HSP7O expression was observed in the brain tissue of normal prenatal and newborn rats. HSP70 was observed in the different regions of hippocampus and cortex from 8h to 168h after hypoxic adaptation. Strongest HSP70 expression was observed in hippocampus CAl . Conclusions HSP70 plays a role in the formation of prenatal hypoxic adaptation.

Objective To evaluate the feasibility and the safety of thulium laser vaporization enucleation of prostate(TVEP) combined with traditional transurethral resection of prostate (TURP) for therapy benign prostate hyperplasia (BPH) with its volume larger than 80 millilitres.Methods Twenty-five BPH patients (volume larger than 80 millilitres) underwent TVEP combined with TURP.The operation time,intraoperative blood loss,enucleation time,cutting time,bladder irrigating time,catheterization time,perioperative and 6 months' follow-up data such as the international prostate symptom score,quality of life score,the maximum urinary flow rate,the residual urine volume and so on were observed.Results The operation time was (66 ± 26) min.The enucleation time was (25 ± 9) min.The cutting time was (32 ± 8) min.The intraoperative blood loss was (140 ± 25) ml.The bladder irrigation time was (3.0 ± 1.0) d.The catheterization time was (5.7 ± 1.0) d.After 6 months,the maximum urinary flow rate,residual urine volume,international prostate symptom score and quality of life score were improved:(18.7 ± 1.7) ml/s vs.(6.8 ± 1.7) ml/s,(18.9 ± 1.8) ml vs.(65.7 ±8.1) m1,(8.7 ± 1.6) scores vs.(25.7 ±4.3) scores,(1.7 ± 1.2) scores vs.(4.7 ± 1.1) scores,and there were significant differences (P ＜ 0.05).Urethral stricture developed in 2 patients and epididymitis happened in 3 patients.No blood transfusion events and transurethral electric cutting syndrome occurred.Conclusion TVEP combined with TURP for therapy BPH larger than 80 millilitres is safe,and the incidence of complications is low.

Objective To investigate the effect of cytosine deaminase(CD)/ 5-FC system in inhibiting growth of LoVo colorectal carcinoma grated in nude mice.Method The retroviral vector pCD2 containing CD suicidal gene was packaged in PA317 cells,the supernatant of recombinant retrovirus was then injected into tumor sites.Result Obvious regression of the tumor occurred after serial intraperitoneal administration of the prodrug for the 5-FC.Conclusion CD gene can be a useful tatic in the comprehensive treatment of colorectal carcinomas.

OBJECTIVE: To study the basic characteristics of three bioactive fungi, the interrelationship of three fungi and the relationship between three fungi and their host, thus to provide data for the theoretical research and the application practice. METHODS: The colonial morphology and microscopic characteristics were studied by light microscopy, and the relationships were studied by agar diffusion method. RESULTS: The basic characteristics of three bioactive fungi were obtained, and the relationships under in vitro conditions were clarified. CONCLUSION: The results of this study will be of great value to the studies and use of the fungi, and it will provide important data for research on the mechanism of fungi inducing Aquilaria sinensis to produce Agilawood.

OBJECTIVE: To investigate the feasibility of utilizing three fungi YNAS04, YNAS06 and YNAS08 to induce the formation of agilawood from Aquilaria sinensis in field and laboratory, respectively. METHODS: Agarwood was obtained by inoculating the fungus in the stem of A. sinensis under natural conditions, and the main ingredients of the volatile oil from induced agilawood were analyzed by GC-MS. The authenticity of the used fungi was detected by the method of morphology and ribosomal internal transcribed spacer. RESULTS: The weights of the induced materials from YNAS04, YNAS06 and YNAS08 were (1.786±0.473), (2964±0.492) and (3.615±0.591) g after six months, which were heavier than blank control (1.325±0.407) g and negative control (1.462±0.417) g, respectively. The fungus inoculated in the stem of A. sinensis and became the dominant fungi in the tissue under natural conditions. Sesquterpenoids and chromone analogues were detected from three fungi inducing materials of six months old. CONCLUSION: Utilizing fungi to induce the formation of agilawood from Aquilaria sinensis has the advantages of spending less time and producing high-quality secondary metabolites. This method has important application foreground in large-scale agilawood production.

OBJECTIVE: To optimize the solid culture condition of three fungi which can induce Aquilaria sinensis to produce agilawood, thus to provide data for the production and application of the fungi. METHODS: Single factor experiment method was applied in the study. RESULTS: The optimal solid culture medium formula, temperature, pH value, moisture, the density of materials and illumination conditions for three fungi were obtained. CONCLUSION: The optimized method can provide high quality fungi for prompting Aquilaria sinensis to produce agilawood at larger scales. Copyright 2012 by the Chinese Pharmaceutical Association.