1.Carotid intima-media thickness and uric acid level and the research of the prognosis of patients with coronary heart disease
Jifeng LI ; Shaowan CHEN ; Yuyang CHEN
Chinese Journal of Primary Medicine and Pharmacy 2015;22(9):1358-1360
Objective To explore the carotid intima-media thickness and the value of uric acid in predicting the prognosis of coronary heart disease patients.Methods Using the random number table method,182 patients diagnosed with coronary heart disease were divided into two groups:group A was 92 cases of acute coronary syndrome (ACS),groups B was 90 cases of chronic stable angina,healthy check-up 90 cases as control group (group C),fasting blood test blood uric acid (UA),application of color Doppler ultrasound measurement carotid intima-media thick ness (IMT).Results Acute coronary syndrome (ACS) set of uric acid,IMT,plaque index were (428.5 ± 70.3) mmol/L,(1.58 ± 0.92) mm,(3.24 ± 1.81) and those in stable angina group were (356.4 ± 68.2) mmol/L,(1.32 ± 0.86) mm,(3.08 ± 1.62) and those of the control group were (251.2 ± 65.4) mmol/L,(0.92 ± 0.41) ram,(2.80 ± 1.18) to compare the difference was statistically significant(group A compared with group B(t =4.47,3.26,5.21,all P < 0.05),group A compared with group C (t =8.23,9.36,7.48,all P < 0.01);group SAP compared with control group the difference was statistically significant (t =6.34,5.26,7.12,all P < 0.05);Linear correlation analysis showed that uric acid and carotid intima-media thickness were positively correlated,r value was 0.67.The ACS group soft carotid atherosclerosis plaque detection rate was 60.9%,SAP group was 26.7% and the control group was 8.9%,the difference was statistically significant (x2 =21.60,P < 0.05;x2 =69.11,P < 0.01),group SAP compared with the control group,the difference was statistically significant (x2 =39.84,P < 0.01).Conclusion Uric acid levels and carotid artery atherosclerosis and coronary artery disease are closely related,which can provide theoretical basis for the risk assessment and prognosis of coronary heart disease.Early intervention can prevent the occurrence of serious cardiovascular events.
2.Glycolysis abnormality in neoplasm cells and the targeted therapy
Yuyang PANG ; Ting WANG ; Fangyuan CHEN
Journal of International Oncology 2012;39(10):726-728
Cancer cells display a decreased mitochondrial respiration along with an abnormal activation of glycolysis to satisfy the demand of the malignant growth even in the presence of oxygen.This phenomenon may be caused by mitochondrial dysfunction,hypoxia of microenvironment and the abnormal activation of upstream regulators of glycolysis pathway.Treatment targeted at the glycolysis pathway and its upstream regulators may provide a new therapeutic strategy of cancer.
3.Relationship Between the Expression of Lymphocyte Function-associated Antigen-1 on Peripheral Lymphocytes and Acute Rejection After Liver Transplantation
Yuyang FU ; Xiaoshun HE ; Jianlin CHEN ; Guihua CHEN ; Jiefu HUANG
Journal of Sun Yat-sen University(Medical Sciences) 2001;22(3):192-194
【Objective】To investigate the relationship between the expression of lymphocyte function-associated antigen-1 (LFA-1) on peripheral lymphocytes and acute rejection after orthotopic liver transplantation (OLTx) in rat.【Methods】Adult male rats were divided into 2 groups.In the non-rejection group,40 SD rats were used as both donors and recipients.In the rejection group,20 Wistar rats were used as donors and 20 SD rats as recipients.Blood samples were collected through the tail vein 1 day before transplantation and on days 1、3、5、7 after OLTx.The expression of LFA-1 (CD11a) on peripheral lymphocytes was analyzed by using indirect immunofluorescent marker-flow cytometry.【Results】The expression level of LFA-1 on peripheral lymphocytes in recipient rats after orthotopic liver transplantation was markedly lower than that before operation (P<0.01);The expression level of LFA-1 on peripheral lymphocytes in rats with acute liver rejection was significantly higher than that in the non-rejection group (P<0.01).【Conclusion】Monitoring the expression level of LFA-1 on peripheral lymphocytes may be helpful to the diagnosis of acute graft rejection.
4.Diagnosis value of expression of ICAM-1 and LFA-1 in acute rejection of liver transplantation in rats
Yuyang FU ; Xiaoshun HE ; Jianlin CHEN ; Guihua CHEN ; Jiefu HUANG
Chinese Journal of General Surgery 1993;0(01):-
Objective To investigate the relationship between the intercellular adhesion molecule-1(ICAM-1) and lymphocyte function associated with antigen-1( LFA-1) and acute rejection of liver transplantation in rats. Methods SD rats and Wistar rats were randomly divided into normal control group, isograft group, and allograft group. Liver biopsies from the recipients were obtained on the day 2,4, and 7 postoperatively to examine the expression level of ICAM-1 and LFA-1 in liver grafts. Samples were firstly stained with specific monoclonal antibodies using immunohistochemistry stain and then applied to image semiquantitive analysis. Results The expression levels of ICAM-1 and LFA-1 in allograft group were obviously higher than those in both isograft group and normal group. Moreover, the expression levels of ICAM-1 and LFA-1 were correlated positively with the degree of acute liver rejection. Conclusions Detecting the expressions of ICAM-1 and LFA-1 in liver graft tissue may be helpful to the diagnosis of acute rejection after liver transplantation.
5.Effects of strict control of tobacco advertisement after implementation of the Advertising Law
Xiaoxia WEI ; Ming LI ; De CHEN ; Yuyang CAI ; Jingrong GAO
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(2):137-140
Objective · To compare the prevalence of tobacco advertisement before and after implementation of the new edition of Advertising Law of the People's Republic of China and evaluate the effects of implementation of the new Advertising Law. Methods · Tobacco sales places, public places, and streets in Shanghai were randomly selected to observe tobacco advertisement and complete the survey. The survey was carried out in 10 districts of Shanghai in June 2015 with 10 tobacco sales places selected in each district, and in 4 districts of Shanghai in June 2016 with 25 tobacco sales places selected in each district. Sales places included convenient stores, tobacco stores, supermarkets, and kiosks. In addition, public transport waiting points, public transport vehicles, hospitals, shopping centers, restaurants, bars, and Internet cafes were selected into survey in 2016. Results · In 2016, the proportion of tobacco sales places having tobacco advertisement was lower than that in 2015, but the proportion of tobacco promotion behaviors was higher. In 2016, 128 public places and streets were investigated and only 1 had outdoor tobacco advertisement. Conclusion · The new edition of Advertising Law has significant effect on controlling tobacco advertisement, but tobacco sales places still have tobacco advertisement with various disguised promotion forms. Tobacco sales places have diversified business styles and minors often enter these places. It is recommended that a clear definition of disguised forms of tobacco advertisement should be made, tobacco advertisement at tobacco sales places needs to be supervised, the release of tobacco advertisement should be strictly reviewed, and existing tobacco advertisement should be severely punished, so as to prevent minors from exposing to tobacco advertisement and information luring smoking.
6.The effects of nano-chitosan/bone morphogenetic protein gel on the repair of mandibular defect
Yuyang CHEN ; Fuqiang XIE ; Yun ZHANG ; Xin WANG ; Jian SUN
Journal of Practical Stomatology 2014;(5):624-628
Objective:To investigate the effects of Nano-chitosan rhBMP-2 Gel(NCS/BMP-2 Gel)on the repair of mandibular de-fect.Methods:NCS/BMP-2 Gel and NCS Gel were prepared and respectively injected into the subcutaneous space on both sides of dorsum of 9 rats.3 rats were respectively sacrificed 10,20 and 30 d after injection.The subcutaneous nodules were histologicaly ex-amined.Mandibular defect was made in 54 SD rats and the rats were divided randomly and into 3 groups(n=18):NCS/BMP-2 Gel was implanted into the defects in group 1,NCS Gel in group 2,no injection in group 3.Animals were sacrificed 3,6 and 9 weeks after transplantation.X-ray examination,pathologic observation were conducted.Results:Subcutaneous nodules were found in both sides of the rat dorsum.The residual mandibular defect area in study group 1 was apparently smaller than that in group 2 and 3(P<0.05). More new bone formation was observed in the gel injected area in group 1 than in group 2 and 3.Conclusion:Nano-CS/BMP-2 Gel is biocompatible and can accelerate the healing of mandibular defect.
7.Effect and mechanism of Gujingdan on acute renal injury induced by gentamicin
Xiulan CHEN ; Li GUO ; Yuyang ZHANG ; Minwei WANG
Chinese Traditional Patent Medicine 1992;0(06):-
AIM:To study the protective effect and mechanism of Gujingdan(Radix Astragali,Fructus Alpiniae oxyphyllae,Radix et Rhizoma Salviae miltiorrhizae,Herba Epimedii,Semen Euryales,etc.) on the acute renal injury caused by Gentamicin in rats.METHODS:90 rats were randomly divided into normal control group,pathologic model group,preventive group which included high,middle and low dosages of Gujingdan(4 g/kg,2 g/kg,1 g/kg) and positive control group which was treated with Shenkangning(2 g/kg) and treated group which included the same groups as the preventive group.The preventive groups were respectively given Gujingdan and Shenkangning for two weeks and ahead one week.On the seventh day,except the normal group the others were continuously injected gentamicin by abdominal cavity(100 mg/kg/day) seven days.At the same time,the treated group were given Gujingdan and Shenkangning by ig for seven days.When the experiment was over,the content of ?-N-acetyglocosamidase(NAG),serum creatinine(SCR) and blood urea nitrogen(BUN) were examined,and the renal histological changes were observed.RESULTS:NAG,BUN,SCR of the groups which were treated with Gujingdan were remarkly lower than that of the pathologic model group;SOD、ATP were higher and MDA was lower than that of the model group.Histological changes showed that renal tubular epithelial cells of the groups treated with Gujingdan were similar to the changes of normal group and much better than that of positive group and pathologic model group.CONCLUSION:Gujingdan has the protective effect on acute renal injury caused by Gentamicin.The mechanism may be that Gujingdan has the function of inhibiting oxidation,protecting and stabilizing cell membrane.
8.Optimization of Extraction Technology of Panax Notoginseng and Scrophulariae Radix from Rupixiao Granule by Orthogonal Design
Hongyu JI ; Yuyang SUN ; Bing SHAO ; Bo CHEN ; Linhua WU
China Pharmacist 2017;20(7):1313-1316
Objective: To optimize the extraction technology of Panax notoginseng and Scrophulariae radix from Rupixiao granule.Methods: With the dry extract rate and transfer rates of ginsenoside Rg1, ginsenoside Rb1 and harpagoside as the comprehensive index, the orthogonal design was adopted to investigate the effects of the amount and concentration of ethanol, extracting duration and times on the extraction technology.The contents of ginsenoside Rg1, ginsenoside Rb1 and harpagoside were determined by HPLC.Results: The optimal extraction technology was extracted twice with 8-fold amount of 60% ethanol with 2 h per time.The transfer rate of ginsenoside Rg1, ginsenoside Rb1 and harpagoside was (79.4%±1.56%), (42.62%±0.68%) and (44.89%±0.58%)(n=3), respectively.The dry extract rate was (20.99%±0.411%).Conclusion: The optimized extraction technology is stable and feasible, which can be used for extracting Panax notoginseng and Scrophulariae radix from Rupixiao granule.
9.Research Progress in Pathogenesis and Traditional Chinese Medicine Treatment of Primary Dysmenorrheal
Yuyang SUN ; Hongyu JI ; Bo CHEN ; Minghui CHU ; Hairong WANG ; Linhua WU
China Pharmacist 2017;20(1):144-147
Primary dysmenorrheal is one of the most common diseases in gynecology,which seriously affects the physical and men-tal health of women, therefore, the effective prevention and treatment of primary dysmenorrheal is a problem in medical field. The etiol-ogy of primary dysmenorrheal is very complicated, and in recent years, there are more and more domestic and foreign scholars studying on its pathogenesis and treatment. Modern medicine has some shortcomings in the treatment of dysmenorrheal including side effects and so on. Traditional Chinese medicine has unique advantages in the treatment of primary dysmenorrheal. Combined with the recent rele-vant reporters, the article reviewed the pathogenesis of primary dysmenorrheal from both traditional Chinese medicine and modern medi-cine aspects, and the research progress in traditional Chinese medicine treatment of primary dysmenorrheal was also reviewed to provide better guidance for the treatment of primary dysmenorrheal.
10.Inhibition Effects of Rupixiao Granules on Contractility of Uterus of Mice in Vitro
Lu ZHONG ; Hongyu JI ; Yuting ZHUANG ; Shuyuan WANG ; Bo CHEN ; Yuyang SUN ; Linhua WU
Herald of Medicine 2016;(2):129-131
Objective To study effects of Rupixiao granules on contraction of uterus of mice in vitro. Methods Mouse model with isolated uterine contraction was established. The mice pretreated with estradiol benzoate were sacrificed and then the uteri were taken out. The normal contractions were recorded by the biological function system. Effects of 10, 20, 30, 40, 50, 60 mg.mL-1 Rupixiao granules on spontaneous and oxytocin-induced (5 U.L-1) uterine contractions were recorded,inhibition of Rupixiao granules on mean constriction amplitude,constriction frequency and contraction activity of pitocin-pretreated uterus was observed and the inhibition rates were calculated. Results Compared with the baselines, the amplitude, frequency, activity of spontaneous uterine contraction were significantly down-regulated by Rupixiao solution at a range of 10-60 mg.mL-1 ,as well as it could inhibit oxytocin-induced spasmodic contraction of isolated uterus at 10-50 mg . mL-1 ( P<0. 01 ) . Conclusion The Rupixiao granules inhibits spontaneous contraction of isolated mouse uteri and oxytocin-induced spasm of uterine smooth muscle in vitro.