Objective:To investigate the expression of sonic hedgehog(SHH) in gastric cancer,analyze the relationship with clinic pathological features,and discuss the clinical significance.Methods:Immunohistochemistry SP method was used to detect the expression of SHH in 60 cases of gastric cancer,and the relationship between protein expression and clinic pathological features was analyzed.Results:The positive expression rates of SHH was 68.3%(41/60)in gastric cancer.The expression rates of SHH was correlated with depth of invasion,lymph node metastasis,and TNM stage(P

Objective: To investigate the role of intraoperative ultrasound(IOUS) and appropriate approach in local resection for the central small hepatocellular carcinoma(sHCC) which is deeply situated within the parenchyma and adjacent to the hepatic veins. Methods: under IOUS guidance, local resection was carried out for 8 cirrhotic patients with central sHCC which was deeply located and adjacent to hepatic veins by approach of splitting or unroofing superficial hepatic parenchyma. Results: All tumors were precisely localized by IOUS and excised successfully. Except for injury to central hepatic vein in 1 case, no other injury occurred to the hepatic veins in the remaining 7 cases. There was no postoperative mortality, and the liver function recovered well in all cases postoperatively. Conclusion: Local resection by using IOUS and appropriate approach is applicable for the cirrhotic patients with deeply located central sHCC. IOUS plays an important role in avoidance of injuries to the hepatic veins.

OBJECTIVE: To discuss therapy regimes and pharmaceutical care for the inpatient with intracranial infection after craniotomy. METHODS: Clinical cases were taken as example and therapy regimes was analyzed in order to put out full range and individualized pharmaceutical care for patients with intracranial infection after craniotomy. RESULTS: Reasonable medication was provided by implementing pharmaceutical care. CONCLUSION: It is necessary to deliver pharmacetical care to the inpatient with intracranial infection after craniotomy.

The mitogen-actived protein kinase(MAPK)is one of important ways in eukaryote signal transmission network,and plays the key role in the gene expression regulation and the cytoplasm function activity.In the mammal organism,five kinds of different MAPK signal transduction passage.ERK1/2 signal transduction passage regulates cell growth and differentiation,JNK and p38 MAPK signal transduction passages play the vital role in the stress of inflammation and apoptosis etc.The activation of signal transduction passage is the typical characteristic of RA chronic synovitis,through this way to induct synoviocytes cytoplasm protein phosphorylation,then causes transcription factor and nucleoprotein c-Fos,c-Jun,AP-1 and NF-kappa B and so on phosphorylation,thus promotes cell proliferation and activation.It has the vital significance of the above-mentioned mechanism research to understand deeply and researche new medicine to RA.

Objective Atrophic gastritis, as a pre-cancerous condition of gastric cancer, is developed from non-atrophic gastritis.The aims of this study was to compare the gene expression profiles between atrophic gastritis and non-atrophic gastritis using cDNA microarray and to explore the molecular mechanisms in the development of atrophic gastritis. Methods Endoscopy and biopsy were performed consecutively in 227 patients (143 male, 84 female, age range between 16-72 years, with average age 48.6 years). Diagnosis of non-atrophic gastritis and atrophic gastritis was made according to histological examination, and 120 patients were in non-atrophic group and 107 in atrophic group. The total RNA was extracted from the biopsy specimens of two groups with Trizol reagent. cDNA microarray consisting 8 464 human genes (HGEC-80s kit) was used, and labeled cDNA with fluorescence probes were hybridized to microarray, and then the comparison of gene expression profiles was made between atrophic and non-atrophic gastritis. Results It was identified that in gene expression profiles of atrophic gastritis there were 165 genes showing a greater than 2-fold increase and 460 genes showing a greater than 50% decrease as compared to non-atrophic gastritis. RT-PCR analysis revealed similar results as in DNA microarray. Conclusions There is different expression in many genes between the gastric mucosal cells of non-atrophic and atrophic gastritis mucosa. Many genes expression profiles are involved in atrophic gastritis that would be of help in further understanding the development of atrophic gastritis.

OBJECTIVE:To study the protective effect of tanshinoneⅡA(TSⅡA)sulfonate injection against vancomycin(VAN)-induced renal injury model rats and its mechanism. METHODS:72 rats were randomly divided into blank group,model group, positive control group(amifostine,1 mg/kg)and TSⅡA sulfonate injection low-dose,medium-dose and high-dose groups(15,30, 60 μg/kg),with 12 rats in each group. Except for blank group,those groups were given VAN(200 mg/kg)intravenously via tail vein to induce renal injury rat model;after modeling,each drug group was given relevant medicine intraperitoneally once a day, and blank group and model group were given normal saline intragastrically for consecutive 10 days. The levels of 24 h protein, NAG and KIM-1 in urine were determined,and those of Cys C,Scr and BUN in serum and those of SOD,MDA,GSH-Px and NO in renal homogenate were also determined;the pathological change of renal tissue was observed. RESULTS:Compared with blank group,the levels of Cys C,Scr and BUN in serum,those of 24 h protein,NAG and KIM-1 in urine and those of MDA and NO in renal tissue increased significantly in model group,while the levels of SOD and GSH-Px decreased significantly(P<0.01);the pathological slice indicated that model group suffered from renal injury such as kidney tubules albuminoid degeneration,brush border abscission,renal tubular epithelial cell disintegration and abscission. Compared with model group,the levels of Cys C,Scr and BUN in serum,those of 24 h protein,NAG and KIM-1 in urine and those of MDA and NO in renal tissue decreased signifi-cantly in treatment groups,while the levels of SOD and GSH-Px in renal tissue increased significantly(P<0.05 or P<0.01);path-ological changes of renal tissue were relieved significantly. CONCLUSIONS:TSⅡ A sulfonate injection can effectively relieve VAN-induced renal injury in rats,and its mechanism may be associated with inhibiting the oxidative reaction of rats in vivo.

OBJECTIVE:To determinate the dissolution of Compound Flavone capsules by HPLC.METHODS:Nova-Pak C18(250mm? 4.0mm,5? m) column was used with column at room temperature.The mobile phase consisted of methanol-0.4% H3PO4(50:50) at a flow rate of 1.0mL? min-1.The detective wavelength was 360nm.The dissolution of the Compound Flavone capsules was determined by basket stirring technique with 0.1mol? L-1 hydrochloric acid as dissolvent at a speed of 100r? min-1.RESULTS:The cumulative dissolution rate of Compound Flavone capsules was above 80% at 30 minutes.The linear range of Quercetin was 0.065 84～ 0.658 4?g(r=0.999 9).The average recovery was 99.35%,RSD=0.92%(n=6).CONCLUSION:The method is simple,accurate and reproducible,and suitable for the guality control of Compound Flavone capsules.

AIM:To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus(DM-1).METHODS:pAd5/PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line.The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting.The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function.The level of IL-2,TNF-? and IFN-? were detected in the cell culture fluid.RESULTS:Compared with the control group,the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2,IFN-? and TNF-?(P

Objective To study the assessment of airway inflammation in asthma patients with airway remodeling by high resolution spiral CT(HRCT).Methods Retrospective analysis on 60 cases of chronicity-persistent period asthma patients with induced sputum IL-4 test,airway mucosa biopsy,HRCT scan; while 20 patients only used HRCT scan as control group.To compare the difference of sputum IL-4,HRCT test of airway wall thickness(WT),wall area(WA)and airway wall relative area(WA％)in different degrees of asthma paitents.Results WA,WA％and WT were significantly increased in different degrees of asthma patients when compared to control group(P<0.05),there were significant difference among each group(P<0.05).WA％and WT were positively correlated with induced sputum IL-4.Conclusion HRCT can effectively evaluate the airway inflammation in airway remodeling of asthma patients,which can be used to evaluate the severity of asthma,treatment effect and follow-up observation.

OBJECTIVE:To study the effect of ligustrazine injection(LTZ)on DNA synthesis of vascular endothelial cell.METHODS:In vitro cultured cell line of human umbilical vein endothelial cells,ECV304,were adopted to measure the ef?fects of LTZ on DNA synthesis by 3 H-TdR incorporation.RESULTS:LTZ could inhibit DNA synthesis of ECV304in a dose-dependent manner.CONCLUSION:The mechanism of anti-angiogenesis of LTS might be associated with inhibiting DNA synthesis of vascular endothelial cell.