Objectives To investigate the effect of electric vagal stimulation on the gene expression of injured myocardium from ischemia/reperfusion rat model and explore the involved molecular mechanism.Methods Twenty Sprague Dawley male rats were randomly selected and divided into 2 groups evenly:ischemia/reperfusion group (I/R group),and vagus nerve stimulation group (STM group).The left anterior descending coronary artery (LAD) was ligated and subjected to 30 min of myocardial ischemia followed by 2 h of reperfusion.In addition,10 min before reperfusion,left cervical vagus nerve of STM group was subjected to electronic stimulation at 5 V,2 ms and 1 Hz for 20 min.After 120 min of reperfusion,every group was randomly divided into two parts.One part that myocardium was collected from left ventricle was applied to determine the area of myocardial infarction.The RNA isolated from another part of the ischemic myocardium collected from left ventricle was hybridized to get gene expression profiles and the quality of hybridized RNA from both I/R and STM group was assessed and analyzed.GeneSpring software was applied to screen out the genes,which show significant difference between groups I/R and STM.Real-time polymerase chain reaction (RT-PCR) was applied to analyze the expression of important genes.Results (1)The area of myocardial infarction of STM (25.5 ± 3.9) ％ was significant reduced relative to L/R group (45.5 ± 4.8) ％ (P ＜ 0.05).(2)The expression levels of 186 genes were changed significantly,and analyzed by Gene ontology (GO) software,there were 3 kinds of genes were affected.The upregulated genes were reported to show protective effect on myocardium.The downregulated gene was relative to inflammation.(3)The RT-PCR result confirmed the genechip assay.Conclusions Electric vagal stimulation can reduce myocardial I/R injury in rats.Significant change of the gene expression was detected between groups I/R and STM.The results suggest that activation of cholinergic anti-inflammatory pathway be involved in the mechanism.

The model of vaginal candidiasis in Kunming mice was constructed in order to search for the optima construction conditions and provide an economic animal model of Candida albicans (C. albicans) vaginitis. Estrogen benzoate (E2) was given to mice at different concentrations ranging from 0.0 to 0.05 mg/mouse (4 levels) beginning 72 h prior to vaginal inoculation, then mice were inoculated intravaginally with various concentrations of stationary-phase C. albicans blastoconidia (ATCC90028) (5 levels) in 20 microL of phosphate-buffered saline (PBS) in each E2 level. General state, scores of genital pathology, the hyphae and vaginal fungal burden (CFU) in vaginal lavage fluid, the hydrops rate of uterus and vaginal tissues for pathological section in mice were observed and obtained at day 2, 4, 7, 14 and 21 after inoculation. The results showed the infection rate in mice was related to the dosage of E2 and concentration of C. albicans blastoconidia. Additionally there was better cross-effect between the two treated factors. The infection rate was about 80% on the day 4, and could reach 100% on the day 7 until the end of experiment after inoculated intravaginally in groups of E2I3, E2 0.025 mg/mouse injected hypodermically and inoculated intravaginally with 5 x 10(4) C. albicans blastoconidia, and large amount of hyphae and blastoconidia could be observe in superficial layer tissue and canal of vaginal by PAS. From the results in our experiment it was concluded that E2I3 was the optima construction condition in kunming mice.

Invasion and metastasis are the distinct biological characterstics of cancer. They are the key factors influencing tumour patients' theraputic effect and prognosis. Recent study shows that metalloproteinases can facilitate tumour cell invasion and metastasis. In this review we evaluate the novel aspects of the function of matrix metalloproteinases in tumor invasion and metastasis. The regulation of the expression and activity of MMPs are summarized. The potential clinical application of metalloproteinase and their tissue inhibitors were also presented.

Objective Quantitative evaluation was conducted to assess the development level and trend,as well as discipline distribution of basic medical research in China,based on SCI publications.Methods Descriptive analysis and trend analysis by 5-year moving time window were applied to analyze the quantity,quality,discipline distribution and current status and trend of development of medical research papers published by Chinese scientists during 2004 and 2013;Results Among a total of 280,200 medical research papers published from 2004 to 2013 period,China ranked # 5 in the world in terms of the quantity of papers.However,the average citation of 8.34 for Chinese paper was far below the global average of 14.11.Among all papers published by Chinese scientists,the percentages of papers in the field of psychiatry/psychology,neuroscience/behavior,immunology and clinical medicine were 2.20％,7.59％,3.93％ and 40.67％,respectively,lower than those of global average (6.71％,9.41％,4.62％ and 47.45％).The percentages of papers in other disciplines were higher for China than for global average.Regarding to the development trend,the annual increase in the number of publications of 5 years was 22.24 ％,while the 5-year citation per paper was only 2.03 ％.Conclusions The quantity of basic medical research in China is large and growing rapidly,while the quality of these researches remains in relatively lower level,and need to be improved substantially.With the discipline distribution different from the global average,psychiatry/psychology,neuroscience/behavior,immunology and clinical medicine should be strengthened in the future.

Objective To study and analyze the efficacy of Metronidazole Suppositories, conjugated estrogen, estrogen ointment and psychological intervention in the treatment of senile vaginitis. Methods 100 cases of senile vaginitis patients in our hospital from February 2015 to August 2016 were selected as the research object. They were randomly divided into the control group and the experimental group, each group had 50 cases. The control group was treated with the treatment of patients in the experimental group of Metronidazole Suppositories, Metronidazole Suppositories, conjugated estrogen ointment in the treatment of psychological intervention on the mental status of patients and patients, strengthen communication and exchanges, increase confidence in the treatment and the treatment compliance of patients. The therapeutic effects of the experimental group and the control group were compared and analyzed. Result After the corresponding treatment, 3 patients in the experimental group were invalid. In the control group, 10 patients were ineffective. The effective rate of the experimental group was 96.0％, which was significantly higher than that of the control group (80.0％), with statistical difference (P<0.05). The incidence of adverse reactions in the experimental group and the control group was 2％ and 4％ respectively, and there was no significant difference. Conclusion Metronidazole Suppositories, combined estrogen ointment and psychological intervention in the treatment of senile vaginitis clinical effect is good, high treatment efficiency, low incidence rate of adverse reaction, has clinical significance.

Benefits in survival and quality of life have been demonstrated in patients with advanced gastric cancer receiving chemotherapy plus optimal supportive care versus optimal supportive care alone.The taxanes, which are among the most promising cytotoxic agents in clinical use have considerable activity in a number of tumor types.Paclitaxel,especially low-dose weekly administration,has shown encouraging activity in the treatment of advanced gastric cancer as a single agent.Patients treated with the low-dose weekly paclitaxel regimen had a statistically superior response rate and time to disease progression as well as clinically significant prolongation of survival.And it offers a new option for those advanced gastric patients such as the aged,poor performance status(PS) or unsuitable for combination chemotherapy,and those even with no indications for rountine chemotherapy.This review is focused on the clinical reports of low-dose weekly administration of paclitaxel for patients with advanced gastric cancer.

Methamphetamine ( METH ) , a powerful stimulant of amphetamine type in the central nervous system ( CNS ) , is a new kind of synthetic drugs mainly in psychological dependence. Long-time abuse will lead to strong neurotoxicity and drug de-pendence. Recently it has become the abuse problem in public health. Autophagy is a process of degradation and utilization of the cellular components by the lysosome, which widely exists in eukaryotic cells. Many studies show that methamphetamine can iduce autophagy,but the underlying mechanism is not fully un-derstood yet . Therefore,the mechanisms and signaling pathways of autophagy by methamphetamine and the roles of autophagy in methamphetamine induce neurotoxicity are reviewed in this pa-per, so as to provide reference materials for further research of methamphetamine and autophagy.

Objective To investigate the Correlation between Plasma Total Homocysteine and Copper in Patients with coronary artery disease complicated with diabetes mellitus. Methods 31 pateints with coronary artery disease complicated with diabetes mellitus were studied. 36 age-matched and gender-matched patients with coronary artery disease not complicated with diabetes mellitus and 30 normal subjects were served as control groups. The concentrations of copper and total homocysteine was determined and compared. Results The concentration of copper and total homocysteine was significantly highter in the patients with coronary artery disease complicated with diabetes mellitus (18.8?3.5 ?mol/L、21.1?3.8 ?mol/L, respectively) than in the patients with coronary artery disease not complicated with diabetes mellitus (15.6?1.9、14.8?2.2 ?mol/L, respectively) (P

Objective To investigate the association of Lipoprotein(a) concentrations and patients with aortic dissection.Methods 27 pateints with aortic dissection were studied. Sixty age-matched and gender-matched patients with acute myocardial infarction and fifty normal subjects were served as control groups. The levels of Serum lipoprotein(a), plasm C-reactive protein, white blood cell count were compared between the three groups. Observed the changes of the levels of lipoprotein(a), C-reactive protein, white blood cell count 24 hours, 72 hours and 5 days after the initial of disease in eight of the patients with aortic dissection. Results 1. Median serum lipoprotein(a) levels of patients with aortic dissection, acute myocardial infarction and normal subjects were 327.35?89.23, 261.35?77.54, 79.69?21.63 mg/L respectively (P

Objective To evaluate the role of procalcitonin (PCT)in the early diagnosis for hand,foot and mouth disease (HFMD)with bacterial infection.Methods Clinical data of 234 HFMD children who were hospitalized be-tween January and July 2012 were analyzed retrospectively,according to discharge diagnosis,data were divided into simple viral infection group (n=178)and viral associated with bacterial infection group (n=56),and data of 20 healthy children were selected as the control group.Serum PCT,C-reactive protein (CRP)and peripheral white blood cell (WBC)count were compared.Results There was significant difference in the level of PCT,CRP and WBC among three groups (F=381.94,24.18,and 26.46,respectively,all P<0.05).The positive rate of PCT,CRP and WBC among three groups was significantly different(χ2=178.25,38.98,and 71.21,all P<0.05),PCT,CRP and WBC in bacterial infection group(92.86%[52/56],85.71%[48/56],and 87.50%[49/56]respectively)were significantly higher than those of simple viral infection group (3.93%[7/178],62.36%[111/178],and 30.90%[55/178]respectively)and healthy control group (5.00%[1/20],10.00%[2/20],and 5.00%[1/20]respectively).The sensitivity rate of PCT,CRP and WBC was 92.86%,85.71%,and 87.50% respectively,specificity rate was 95.00%,90.00%,and 95.00% respectively.Conclusion The level of PCT has important value for the early diagno-sis of HFMD with bacterial infection,and its accuracy rate and sensitivity are better than CRP and WBC levels.