Objective: To study and analyze the influence of atorvastatin combined trimetazidine on levels of blood lipids,inflammatory factors and cardiac function in patients with coronary heart disease (CHD).Methods: A total of 128 CHD patients treated in our hospital from May 2015 to Oct 2016 were selected.According to random number table, they were randomly and equally divided into trimetazidine group (received trimetazidine based on routine treatment) and combined treatment group (received atorvastatin based on trimetazidine group).Both groups were treated for three months.Levels of blood lipids, inflammatory factors and cardiac function were measured and compared between two groups before and after treatment.Results: Compared with trimetazidine group after treatment, there were significant reductions in levels of triglyceride [(2.11±0.73) mmol/L vs.(1.83±0.77)mmol/L], total cholesterol [(5.14±0.96)mmol/L vs.(4.35±0.73)mmol/L], low density lipoprotein cholesterol [(2.35±0.68) mmol/L vs.(1.90±0.34) mmol/L], N terminal pro B type natriuretic peptide [(296.61±28.96) pg/ml vs.(187.53±32.63) pg/ml], fibrinogen [(4.44±0.34) g/L vs.(3.63±0.54) g/L] and high sensitive C reactive protein [(2.41±0.96) mg/L vs.(1.96±0.82) mg/L], left ventricular end-diastolic dimension [(59.25±6.17)mm vs.(48.43±4.28)mm] and left ventricular posterior wall thickness [(11.01±1.08)mm vs.(9.05±1.04)mm], and significant rise in level of high density lipoprotein cholesterol [(1.32±0.25)mmol/L vs.(1.47±0.38)mmol/L] and left ventricular ejection fraction [(41.28±7.04)% vs.(48.66±7.54)%] in combined treatment group, P<0.05 or <0.01.Conclusion: Atorvastatin combined trimetazidine can significantly improve blood lipid, inflammatory factor levels,cardiac function and cardiac structure in CHD patients.The therapeutic effect is significant, which is worth extending.

OBJECTIVE: To investigate the characteristics and influence factors of sensitization hy allergens in patients with allergic rhinitis in the junction of Guangdong and Guangxi provinces. METHOD: One thousand one hundred and twenty-four patients who were diagnosed with allergic rhinitis hy clinical history and symptoms underwent a skin prick test. The percent of allergens with positive skin response and Multiple Allergens Positive Scores were recorded, and their influence factors, included age, gender and test period were evaluated. RESULT: The percent of top three inhalant allergens with positive skin tests were as follow: dermatophagoides pteronyssinus (77.0%) ,dermatophagoides farina (77.1%), blattella germanica (42.3%); the percent of top three ingested allergens with loositive skintests were as follow: crab ( 39.5%), shrimp (31.1%), ribbonfish (10.8%). The average Multiple Allergens Positive Score was (3.6 +/- 2.7). The percent of allergens with positive skin response and Multiple Allergens Positive Score of different allergens changed as age, gender and text period changed, and the changing trend of them was accordant. The percent inhalant allergens with positive skin tests in dermatophagoides pteronyssinus, dermatophagoides farinae and cat fur in different gender showed a significant difference. CONCLUSION Acarine allergens were the most common allergens in patients with allergic rhinitis in the junction of Guangdong and Guangxi provinces. The percent of allergens with positive skin response and the severity of multiple allergy were influenced by age, gender and text period. The severity of multiple allergy can be measured by Multiple Allergens Positive Score.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Allergens ; analysis ; immunology ; Child ; Child, Preschool ; China ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; diagnosis ; immunology ; Skin Tests ; Young Adult

Objective To establish a human umbilical artery EC-SMC co-culture model, and mimic the morphological and functional characteristics of human arterial wall, for further reseach of the pathological mechanism and therapy of atherosclerosis and imflammatory damage. Methods We secceeded in the primary culture of human umbilical artery endothelial cells (HUAEC) and human umbilical artery smooth muscle cells (HUASMC) by collagenase perfusion digestion and tissue planting, respectively. HUASMCs were incubated in a medium with ascorbic acid at the concentration greater than 50 μg/mL to produce collagen, which was considered as the extracellular matrix for ECs. Then HUAECs were seeded directly upon HUASMCs in a saturate density for sufficient direct physical interaction between ECs and SMCs. The morphological characteristic of EC-SMC co-culture was identified by immunofluorescence staining, and the function of EC-SMC co-culture was identified by Dil-Ac-LDL uptake test. Results The morphological identification showed that the entire surface of HUASMCs was covered by a confluent monolayer confluent monolayer, which indicated that the model had simulated the morphological characteristic of human arterial wall. The results of Dil-Ac-LDL uptake test showed that there was a fluorescent signal in HUAECs. Compared with EC monoculture, the Dil-Ac-LDL uptake of HUAECs was increased significantly in the co-culture system. All the reseach results indicated that there was an interaction between HUAECs and HUASMCs in the co-culture system. Conclusions In the present study, human umbilical artery EC-SMC co-culture model was constructed successfully, which could mimic the morphological characteristic and basic functions of human arterial wall.

In order to study the influencing factors of clinical teaching quality,students studying in non-affiliated hospitals,clinical teachers,teaching management personnel in hospitals and university were investigated as study subjects with Delphi Method.Suggestions were put forward that non-affiliated hospitals should improve the construction of the teaching staff,emphasize the teaching management,and strengthen students' education and management for improving clinical teaching quality in non-affiliated hospitals.

This paper discussed the importance and urgency of enhancing medical students' law education through analyzing the status of student' s knowledge on the medical law,changes in the environment of physician practicing and damages and disputes between the doctors and patients made by the student' s lawbreaking.Meanwhile,suggestions were proposed including establishing a law teaching group consisted of professional and pluralistic teachers,disseminating the law concept,adopting appropriate methods in appropriate periods.

Pancreatic cancer is a commonly malignant gastrointestinal tumor with an obviously increasing incidence all over the world.Those patients without specific symptoms at early stage had mostly lost the opportunity of surgical therapy when pancreatic cancer was detected at advanced stage,and their prognosis is poor.Therefore,it is rather crucial to improve the early diagnosis of pancreatic cancer.Tumor marker is a considerable tool for early tumor detection and screening.It will help to improve the diagnostic rate of early pancreatic cancer and promote the prognosis of pancreatic cancer if we could find out tumor markers and screen one or a group of pancreatic cancer tumor markers.

Objective To explore the relationship between high-sensitivity C-reactive protein (hs-CRP) and the occurrence of certain diseases such as coronary heart disease (CHD) and acute myocardial infarction (AMI).Methods Sixty-one CHD patients, 45 AMI patients (patients group, excluding high hs-CRP level diseases caused by other etiological factors) and 35 healthy controls (control group) were enrolled in the study. The serum level of hs-CRP and myocardial zymogram was measured in the Hitachi 7060 automatic analyzer by latex-enhance immunoturbidimetry for all subjects, and cTnI of AMI group was detected with immunofluorescence method at the same time. The difference of hs-CRP level was compared and analyzed between control group and patients group; control group before and after treatment. Moreover, the relationship between hs-CRP and myocardial zymogram as well as cTnI was analyzed.Results The level of hs-CRP was significantly higher in patients group than that in control group (P<0.01). There was statistical difference of hs-CRP level before and after treatment in control group (P<0.01). hs-CRP was positively correlative with myocardial zymogram and cTnI.Conclusion The serum level of hs-CRP is significantly elevated in CHD and AMI patients, indicating the measurement of hs-CRP contributes to auxiliary diagnosis, therapeutic effect observation, and prognosis evaluation.

Objective To investigate the effect of losartan,an angiotensin 1I type 1 receptor antagonist(ATlRA),on transforming growth factor-β1 expression in alcoholic nephropathy in rats.Methods Male adult Wistar rats were random divided into three subgroups and treated daily for 12 weeks as follows,rats were treated with normal saline[20ml/(kg·d)gavage twicely]in vehicle group,rats were treatedwith alcohol[400g/L,8g/(kg·d)gavage twieely]in alcoholic nephropathy,and rats were concomitantly treated with alcohol and Iosartan [200mg/L,ling/(kg·d),gavage]in alcohol+losartan.HE staining,Sirius red staining,electronic microscope,and immunohistochemistry were used to examine histologic changes,collagen fibers deposition,and the expression of TGF-BI.Results Compared with vehicle treated rats,alcoholic nephropathy rats showed tubular vacuolation,granular degeneration,swelling,tubulointerstitial inflammatory cell infil tration,and rarely mesangial proliferation and matrix deposition.Sirius red staining displayed a significant increase in collagen fibers in alias of glomemli,tubulointerstitium and vessels.Concomitant administration of losartan reversed all of above parameters(P<0.05).The upregulated expression of TGF-β1 proteins was significantly suppressed by losartan treatment(P<0.05).Conclusions These findings suggest that alcoholic nephropathy is characterized by the tubular granular degeneration,vacuolation,swelling,and tubulointemtitial inflammato-ry cell infiltration,and this is associated with upregulation of TGF-β1.Losartan treatment offers protection effect by downregulating TGF-β1 expression.

As an important inflammatory cytokine, tumor necrosis factor-α (TNT-α) plays an important role in the process of cerebral ischemia-reperfusion. The discussion of the dynamic changes, the mechanism of neurotoxicity and antagonizing the therapeutic effect of TNF-α in the process of cerebral ischemia-reperfusion injury will provide theoretical basis for the treatment of cerebrovascular diseases.

The significance of strengthening the construction of teaching staff in non-affiliated hospitals was discussed. Combined with the problems in strengthening the construction of teaching staff, some countermeasures were put forward, such as emphasizing on teaching, enhancing teaching awareness, improving teaching institutions and teaching rules, selecting teaching staff with high standards, strengthening teacher's teaching ability, establishing teaching quality guarantee system and teaching stimulating mechanism.