Objective To evaluate the treatment outcome of laparoscopic Roux-en-Y gastric bypass on old patients (≥60 years old) 3 years after surgery.Methods The clinical data of 116 patients who underwent LRYGB from Jan.2011 to Mar.2014 were retrospectively examined.Patients were divided in two group:group≥ 60 years comprised of 46 patients,group ＜60 years comprised of 70 patients.Preoperative and postoperative weight,blood glucose,blood lipid,hypertension and complication were evaluated,and the efficacy on the two groups was compared.Results The average age of group≥60 years was (63.1±2.5)years and the mean duration of diabetes was (11.3±4.8) years.Mean weight decreased from (81.8±12.7)kg to (64.6±11.7)kg (P＜0.01),mean BMI decreased from (30.5±3.8) kg/m2 to (24.0±4.1) kg/m2 (P＜0.01),glycosylated hemoglobin(HbA1c) decreased from (9.0±2.0)％ to (7.06±126)％ (P＜0.01),and the remission of diabetes was 45.7％.Significant difference was also found in weight,BMI,EWL％,FBG,PBG,HbA1c,HOMA-IR,Ch,TG,LDL-C,hypertension at 3 years after surgery (P＜0.05),but HDL-C level was similar after the operation.There was no significant difference between group≥60 years and group ＜60 years(P＞0.05) except HbA1c(P＜0.05).The incidence of postoperative complicationswasnot statistically significant(P＞0.05).Conclusions LRYGB is a safe and effective treatment for old patients with obesity and T2DM.The remission rate of T2DM 3 years after surgery was similar between the 2 groups.

OBJECTIVE To explore the intracellular expression changes of p38 and Erk1/2 under three phototoxic conditions, compare three detection methods, and further investigate their molecular mechanisms. METHODS The methods involved using three testing approaches to assess the phototoxicity of chlorpromazine(CPZ) and tiaprofenic acid(TA). Subsequently, whole-cell lysates from the biological samples used in the three testing methods were collected, and the changes in the expression levels of intracellular p38 and Erk1/2 were evaluated using Western blotting. RESULTS All three testing methods accurately identified CPZ and TA as phototoxic compounds. In the 3T3 NRU phototoxicity assay, compared to the control group, the expression of p38 significantly increased under phototoxic doses of TA and CPZ(P<0.05), a phenomenon not observed in other testing methods. In phototoxicity assays using guinea pig and artificial skin models, similar expression pattern changes were observed for p38 and Erk1/2. CONCLUSION p38 and Erk1/2 have obvious dose dependence and high sensitivity in the 3T3 NRU phototoxicity test, and can be considered as potential molecular markers for evaluating the phototoxicity of 3T3 NRU. However, they have not been feasible in guinea pig tests and EpiKutis artificial skin tests. Guinea pigs and EpiKutis artificial skin exhibit more similar changes in p38 and Erk1/2 expression, suggesting that the EpiKutis artificial skin model test method may be a better alternative to animal testing than the 3T3 NRU method.

OBJECTIVE To compare the anti-thymic atrophy effects of petroleum ether(PEE), ethyl acetate(EAE), n-butanol(BE) and water extract(WE) fractions from root tubers of Tetrastigma hemsleyanum Diels et. Gilg based on mice thymic atrophy model established by intraperitoneal injection of PolyI:C to preliminarily investigate the mechanisms of its effects. METHODS One hunderd ICR mice were randomly divided into 10 groups: control group, model group, low- and high-dose groups of the four extract fractions, with 10 mice in each group. The control group and model group were given equal volume of purified water and gavaged for 10 d. Starting on the 9th day, PolyI:C solution of 16 mg·kg−1 was injected intraperitoneally for 2 consecutive days, except for the control group, which was injected intraperitoneally with physiological saline. Samples of thymics were weighed and and visceral index was calculated, blood samples were taken for analysis of IL-2, TNF-α and WBC, thymic histopathology was analyzed using hematoxylin-eosin staining, Western blotting was performed to detect the expression of TNF-α, p-NF-κB and NF-κB. RESULTS Compared with the model group, all groups of extracts could enhance the thymus weight and thymus index, as well as the cortex and medulla area ratio and the number of thymic corpuscles in 1 mm2 of medulla to different degrees, and at the same time, significantly reduce the level of TNF-α in plasma, evidently inhibited the phosphorylation of NF-κB, and reduced the protein expression of TNF-α, and each of them having distinct advantages and disadvantages. Peripheral blood white blood cell, lymphocyte absolute count and lymphocyte percentage were elevated in the low-dose WE group, low-dose EAE group and high dose PEE group, IL-2 levels were significantly elevated in the low-dose WE group and high dose BE group, and these effects were particularly well demonstrated. CONCLUSION Four extracts from root tubers of Tetrastigma Hemsleyanum Diels et. Gilg can improve morphological changes and atrophy of the thymus tissue in different degrees, regulate of peripheral blood leukocyte imbalance in post-modeling mice, it may be related to the inhibition of PolyI:C-induced phosphorylation of the NF-κB and reduction of protein expression of pro-inflammatory factors.