Objective: To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray,and provide evidences for clinical treatment and prognosis. Methods: Immunohistochemical staining by S-P technique was used for detecting the expression and distribution of perforin and granzyme B in lymphoma tissue microarray,composed of 60 samples of lymphoma tissue.10 NK/T-cell lymphoma routine sections were used for relative research,and 10 reactive hyperplasia were used for comparison. Results: In the tissue microarray,samples originated from intranode and extranode were 48 and 12,respectively;consisting of 42 B-cell lymphoma,16 T-cell lymphoma(10 PTCLs,2 NK/T-cell lymphomas,2 lymphoblastic T-cell lymphomas,2 anaplastic large cell lymphomas),2 Hodgkin's disease.42 samples of B-cell lymphoma cells were negative in perforin and granzyme B.In 10 samples of peripheral T-cell lymphoma,perforin and granzyme B positive were 8 and 9,respectively,but the positive cells were no tumor cells.In 12 samples of NK/Tcell lymphoma(2 in the tissue microarray,10 routine sections),both perforin and granzyme B were strongly positive.B-cell lymphoma,T-cell lymphoma and NK/T-cell lymphoma differed significantly(P

Recently,tissue-engineered chondrocyte transplantation has been tried to treat full-thickness cartilage defects.Of the many scaffold materials being investigated,collagen,with good biocompatibility,biodegradable and biological activity,has been shown to have many advantageous features for the proliferation,the attachment and the differentiation.The present article reviews the applications and prospects of collagen in cartilage tissue engineering.

Severe clubfoot has complicated clinical features.Conventional surgical management could hardly get satisfactory results,always with serious trauma,and even smaller foot.This paper summarizes the characteristics of clubfoot in details and its relative assessment standard,principles and advantages of Ilizarov's technique,design of the apparatus and correction procedures.Combination of this and traditional technique and reasonable osteotomy method are also analyzed,complications are discussed for prevention.

Objective To construct an eukaryotic expression vector of pre-miR-15a,and to investigate the inhibitory effect of pre-miR-15a to Raji cells. Methods The pGCSIL-GFP vector encoding pre-miR-15a nucleotides was transfected into the bacterial competent cells,and then confirmed by PCR and sequencing analysis. The identified vector was transfected into Raji cells with oligofectamine 2000. The cells were divided into 3 groups,blank,negative control and pre-miR-15a group. Semi-quantitative RT-PCR was used to detect the expression of Bcl-2 mRNA,and immunofluorescence indirect for Bcl-2 protein expression. The growth of Raji cells was assayed by trypan blue dye exclusion method. Results PCR and sequences analysis indicated that the recombinant clones was identical with target sequences. Many green fluorescent cells were observed under fluorescent microscopy. The levels of Bcl-2 mRNA at every group had no obviously difference. Bcl-2 protein expression was obviously decreased at pre-miR-15a group compared with the other groups. Trypan blue dye exclusion method showed the cell growth was inhibited at 48 h and 72 h post-transfection. Conclusion We successfully construct the eukaryotic expression vector of pre-miR-15a,and it can inhibit the growth of Raji cells.

Islet transplantation is one of the effective methods for diabetic therapy. Insulin-producing cells oriently differentiated from embryonic stem cells provide enough cell sources for islet transplantation. The article reviews the progress of recent research on differentiation of embryonic stem cells into insulin-producing cells.

The aetiology of inflammatory bowel diseases(IBD) is still unknown at the present time.However,along with more and more experimental models of IBD developed in recent ten years,the therapeutic effect of probiotics on IBD and the possible mechanisms were widely explored.A lot of experiments have shown that probiotics administration can significantly ameliorate the IBD in many kinds of animal models and this beneficial effect of probiotics may be associated with inhibiting microbial growth,enhancing gut-barrier function,modulating immune response of intestinal mucosa and decomposing luminal pathogenic antigens.

Allopurinol is a xanthine oxidase inhibitor,it can reduce production and excretion of uric acid and oxygen free radicals generation. Allopurinol has good therapeutic effects on gout and hyperuricemia,and can relief symptoms of prostatitis. In addition, allopurinol has protective effects on ischemical reperfusion injury,inflammation and tumor.

OBJECTIVE To provide evidence for decision on the risk factors of nosocomial infection in diabetes.METHODS To search CMBdisc and CHKD supplemented by information from trial registers,8 epidemiological studies on the risk factors of nosocomial infection on diabetes from 1997 to 2004 were analyzed synthetically by meta analysis.RESULTS Through analysis age,course,hospitalization days,blood sugar,wound check,and(antibiotics) utilization were of the significant statistical difference.Risk,sex and complication were of no significant statistical difference.CONCLUSIONS The factors of nosocomial infection on diabetes are age,course,(hospitalization) days,blood sugar,wound check,and antibiotics utilization.But this meta analysis only included domestic(papers).There need enough evidence to support the result and may justify further research.

Objective: To improve the language teaching ability of teachers.Method: The teachers younger than 35-year-old youth from the Premedical courses were selected for observation of teaching,and they were divided into control and experimental classes in teaching language classes for quantitative evaluation skills.Result: The distribution of scores of the classes focused on the 80 to 95 between classes and the scores were mostly concentrated in between 65 to 80.P

Assurance system of Medical Quality is the core of teaching quality assurance work from colleges itself.This paper analysed the major factors affecting the teaching quality such as teachers,students,the teaching environment,teaching management based on quality management theory,making use of information technology to support the implementation of quality assurance system,constructing a system composed of five quality security system framework and forming protection of the quality of teaching based on information.