Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Cardiovascular Diseases ; Cardiovascular System ; Diet, Vegetarian ; Humans ; Integrative Medicine ; Vegetarians

Humans ; Hypertension ; therapy ; Integrative Medicine ; Medicine, Chinese Traditional

Angina, Unstable ; diagnostic imaging ; therapy ; Angioplasty, Balloon, Coronary ; Coronary Angiography ; Humans ; Myocardial Infarction ; diagnostic imaging ; therapy ; Randomized Controlled Trials as Topic

Health Services ; Humans ; Medicine, Chinese Traditional ; Professional Practice

Critical Pathways ; Integrative Medicine

Academies and Institutes ; organization & administration ; China ; Combined Modality Therapy ; methods ; trends ; Drugs, Chinese Herbal ; therapeutic use ; Education, Medical, Continuing ; Humans ; Integrative Medicine ; legislation & jurisprudence ; trends ; International Cooperation ; Medicine, Chinese Traditional ; methods ; trends ; Western World

Esophageal Neoplasms ; mortality ; surgery ; Humans ; Quality of Life ; Survival Analysis

Strokc is one of the most feared perioperative complications.This article systematically reviews the incidence, mechanisms, risk factors stratification,and relief of perioperative stroke.Aiming at the risk factors and pathogeneses,it is hopeful to improve from the aspect of operative methods.techniques,and medication,and thus to decrease the risks of perioperative stroke and improve prognosis.

The morbidity and mortality of intracerebral hemorrhage (ICH) are very high. Brain injury involves in several mechanisms after ICH, including the direct tissue destruction by the mechanical force on brain tissue surrounding the hernatoma in the process of hematoma formation, the release of clot-derived factors (thrombin, hemoglobin degradation products), inflammatory response and complement cascade reaction, etc. This article reviews the above mechanisms