Being one of the main choices for treatment of lung cancer, surgery has developed enormously. Minimally invasive treatment of lung cancer includes video-assisted thoracoscopic surgery(VATS) and Muscle-sparing thoracotomy (MST) which are both feasible and safe for lobectomy, and both with less bleeding, less impairment of pulmonary and heart function, quicker return to full preoperative activities with less pain, fewer complications, and shorter length of stay mhospital than full postero-lateral thoracotomy. Muscle-sparing thoracotomy is simple and safe, providing adequate exposure,satisfying radical resection. So it could be the routine incision in resection of carcinoma and benign tumors in lung. Lacking prospective randomized study data, and with difficulties in dissecting mediastinal lymph nodes, and no difference in postoperative complications, length of stay in hospital, and survival rate, VATS could only be applied to selected patients for ‘easy’ lobectomies; but MST could be used in almost all the cases of lung cancer surgery.

Recent studies have shown that the loss-of-function LKB1 mutations occur in approximately 30% of human non-small cell lung cancer(NSCLC). Studies from lung cancer animal models have demonstrated that functional loss of LKB1 dramatically promotes lung cancer invasion and progression, underscoring the essential role of LKB1 as a potent tumor suppressor involved in lung cancerigenesis, differentiation and metastasis. However, the relationship between loss-of-function LKB1 mutations and lung cancer prognosis remain largely unclear, therefore, we summarized the progress of the research on LKB1.

Surface-enhanced laser desorption ionization spectrometry (SELDI),as a new type of proteinchip technique,could easily and quickly acquire the information of proteins in all kinds of body fluid and tissue. It could be used in selecting new tumor markers,early diagnosis and evaluating the risk of tumors. This article introduces the principium of this ProteinChip technique,its application in clinic detection,and the outline of its future.

Bronchoscopy is a minimally invasive method for obtaining peripheral pulmonary lesions (PPL). Traditional bronchoscopy-guided transbronchial lung biopsy (TBLB) is performed under X-ray guidance, and diagnostic rate is relatively low. A new, real-time electromagnetic navigation bronchoscopy (ENB) is a minimally invasive diagnostic technique which appeared in recent years. Studies suggest ENB is a feasible and safe method for diagnosis of PPL which shows higher diagnostic yields than traditional TBLB, and its potential application in localization and treatment of PPL. This article reviews the clinical application of the technique.

Objective To investigate the feasibility of minimally invasive muscle-sparing thoracotomy for gastric cardia tumor. Methods Minimally invasive muscle-sparing thoracotomy through the left 7th intercostal space was performed in 25 patients with gastric cardia tumor. Results Proximal gastrectomy and lower esophagectomy combined with esophagogastroanastomosis was performed in 20 patients with adenocarcinoma of gastric cardia and 4 patients with squamous cell carcinoma. Gastric fundus incision and tumor resection was conducted in 1 patient with hyperplastic polyps of gastric cardia. No perioperative mortality or serious complications were seen. Conclusions Minimally invasive muscle-sparing thoracotomy is simple and offers good exposure, being an alternative for carcinoma of gastric cardia in relatively early stages.

Objective: To explore the effectiveness of ethanol precipitation in depleting the human serum albumin. Methods: Serum samples from 8 healthy controls and 8 lung adenocarcinoma patients were treated with 100% ethanol to deplete the albumin.The results of the albumin depletion were analyzed by 1-D gel electrophoresis and albumin quantitation. Results: After ethanol treatment,1-D gel images of the serum samples showed a near depletion of albumins a presence of low-abundance proteins.There was no difference in the 1-D gel images between the normal and lung adenocarcioma serum samples.The average concentrations of albumin in the ethanol-treated serum samples were(5.88 ? 0.35) g/L and(5.50 ? 0.53) g/L respectively in the control and lung adenocarcinoma groups.Conclusion: Ethanol precipitation can deplete most of the human serum albumins and thereby assist us in further analysis of human serum proteomics.

Objective To explore the feasibility and short-term efficacy of combined use of laparoscopic and thoracoscopic Ivor Lewis esophagectomy for the treatment of esophageal carcinoma.Methods A case of esophageal carcinoma was treated in our hospital in December 2007.Five trocars were used via laparoscopy to free the stomach and create a tube-like stomach.Afterwards,thoracoscopy was carried out via 4 trocars to remove the lesion and open the diaphragm,and then the tube-like stomach was pulled into the chest cavity and anastomosed to the chest wall.Results The operation time was 330 min,and the intraoperative blood loss was 200 ml.The lesion was removed completely with negative cutting edges.Postoperative pathological examination showed squamous cell carcinoma(stage T2N0M0).Follow-up was available for 3 months,during which no recurrence was found.Conclusion Laparoscopic and thoracoscopic Ivor Lewis esophagectomy is feasible and safe for the treatment of esophageal carcinoma.

Objective To investigate the infection of chlamydiae trachomahs(CT) and myoplasmas in urogenital tract and antibiotic susceptibility of cultured genital myoplasmas.Methods 5095 patients with urogenital tract infection were detected with mycoplasma identification susceptibility testing reagent kit,and the drug susceptibility to eight antibiotics of ureaplasma urealyticum (Uu) and mycoplasm hominid (Mh) were tested by broth microdilution method.And chlamydiae trachomatis was examined by golden standard method.Results In 5095 cases, 417cases(8 2%) were infected with chlamydiae trachomatis, and the infective rate in woman(11 2%) was statistically higher than that in man(6 2%). 1728 cases(33 9%) were infected with mycoplasma, and the infective rate in woman(43 0%) was statistically higher than that in man(28 0%).The cases infected with simple UU(1251,24 6%) were more than that in the cases infected with simple Mh(71,1 4%) and the mixed infected cases(406,8 0%). Drug sensitivity to erythromycin, roxithromycin, josamycin, azithromycin, doxycycline, minocin,ofloxacin, ciprofloxacin in Uu infection were 60 3%,67 5%,73 2%,85 3%,55 7%,40 1%,25 6%,2 7%,respectively;while the mixed infection of Mh and Uu had resistance to the eight antibiotics on the different degree.Conclusions The infective rate of chlamydiae trachomatis and myoplasma in urogential tract and the resistance rates to 8 antibiotics in Hunan province were in a higher level, compared with other area inland. It is necessary to develop antibiotic susceptibility test,in addition to the myoplasma culture for guiding the clinical therapy.

Objective:To observe the up-regulation of nuclear Clusterin (nCLU)gene on the biological behaviors of human non-small cell lung cancer cell line A549 .Methods:Sense eukaryotic expression vector of nCLU was constructed by cloning the cDNA of nCLU into pIREShyg3 vector. A549 cells were transfected with pIRES-nCLU and pIREShyg3 vectors by lipofectAMINE~(TM) 2000 mediation, respectively. Stable transfected cells were selected by hygromycin B screening. CCK-8 assay was performed to evaluate the effect of nCLU over-expression on cell proliferation in vitro. The expression level of nGLU protein was examined by Western blotting. Cell cycle distribution was detected by FCM with PI staining. The alteration of migration and metastasis potential of A549 cells before and after nCLU gene transfection were assayed by cell chemotactic migration and invasion test. Results:The proliferation speed of the transfected A549 cell clones stably over-expressing nCLU was slowed down. FCM analyses revealed that the percentage of cells in G_0/G_1 phase dramatically increased from (33.54±2.10)% to (63.31±4.30)%. The cell chemotactic migration and invasion potentials were markedly reduced after nCLU gene transfection (P<0.05). Conclusion:Up-regulation of nCLU can greatly inhibited the proliferation and decreased the migration and invasion capabilities of A549 cells.

Objective:To analyze the incidence trend, current status and survival period of young patients with lung cancer aged from 15 to 44 years in Shanghai between 2002 to 2005. Methods:All the data were collected from the database of registration and ma-nagement system of diagnosed cancer cases in Shanghai city organized by Shanghai Municipal Center for Disease Control & Prevention. The incidence trend was analyzed by using APC(annual percent change)model. The survival period and median survival time were calculated by using SPSS 12.0 software and life table method. The univariate and multivariate analyses were analyzed by using Kaplan-Meier and COX regression model, respectively.Results:A total of 972 young patients with lung cancer who aged from 15 to 44 years were diagnosed in Shanghai between 2002 and 2005, accounting for 3.09% of the whole percentage of adenocarcinoma in young patients was higher than that in whole lung cancer population at the same period, while the TNM stage was not significantly different between male and female young patients. Multivariate analysis demonstrated that gender, pathological classification and TNM stage were independent prognostic factors for young patients with lung cancer. Conclusion:The lung cancer had different characteristics in young patients compared with that in whole lung cancer population in Shanghai city. It was necessary to further investigate the biologic behavior of lung cancer in young patients.