AIM: To investigate the effect of binocular vision training on stereopsis establish and visual function in child concomitant strabismus after surgery
METHODS:Ninety-three cases of strabismus children were randomly divided into two groups, making it comparable. The control group (n=46) were treated with conventional surgery and care;the observation group ( n=47) on the basis of routine care as control group, were given binocular vision training. Synoptophore and Titmus stereoscopic view were used to check the function of stereoscopic.
RESULTS:After treatment, simultaneous perception, visual fusion and stereopsis of trail patient were 77%, 62% and 40%, respectively, those of control group were 48%, 35% and 18%, the difference was statistically significant (P<0. 05). After treatment, fovea stereopsis and macular stereopsis children in observation group were accounted for 32% and 26%, respectively, in the control group those were accounted for 18% and 11%, respectively. Those in the observation group were significantly more than the control group. No stereoscopic patients in observation group were 23%, and that was 54% in the control group. That in the observation group was significantly less than the control group. The difference comparison between the two groups was statistically significant (P<0. 05).
CONCLUSION: Binocular vision training can effectively help children to build concomitant strabismus after surgery, for children with strabismus should be treated with conventional visual training to improve visual function and improve the life quality.

Optic neuritis is a common optic neuropathy,which typically presents with sudden loss of vision.Visual deficit of optic neuritis varies in severity and generally can be improved spontaneously over several-month duration.The most convincing eight evidences from level Ⅰ and Ⅱ evidence of evidence-based medicine( EBM ) about the treatment of all the optic neuritis were collected by searching theCochrane Controlled TrialsRegister (CENTRAL),MEDLINE,EMBASE and reference lists of identified trial reports since the last decade.At present,the latest findings showed that there is no conclusive evidence of benefit in terms of recovery to normal visual acuity,visual field or contrast sensitivity with either intravenous or oral corticosteroids at the doses evaluated in trials included in this review.Either no treatment or intravenous corticosteroid therapy followed by oral corticosteroids is appropriate,and intravenous corticosteroid may benefit the patient in terms of faster recovery to normal vision.There may be of different treatment of optic neuritis in China compared to Western countries due to geographical,racial and genetic background that may cause different etiology and clinical features of optic neuritis.Therefore,in China,it is imperative to carry out a large randomized control trial(RCT) of optic neuritis as soon as possible to get the best evidence for the management of optic neuritis patients.

OBJECTIVE To monitor the hand contamination after dressing change for the infected wound and to explore an effective way for hand hygiene. METHODS Caregivers were divided into three groups after applying dressing on infected wound:group 1(no hand-washing),group 2(regular hand-washing),and group 3(standard hand-washing),the pathogens in hands were monitored and the effects of two ways of hand-washing were compared. RESULTS The rate of hand-carrying pathogens after dressing change for the infected wound was 100.0%,the qualified rate of regular hand-washing was only 47.9% and that of standard hand-washing (six-steps method)was 97.9%. CONCLUSIONS The standard hand-washing is more effective than regular hand-washing. Correct hand hygiene should be emphasized to prevent and control nosocomial infection.

Based on the domestic and international research in the past few decades,we present an intelligent non-destructive diagnostic instrument for coronary artery disease(CAD).This instrument analyses the multi-channel heart sounds of patients who have coronary artery disease and combines other information about CAD,then wavelet analysis,artificial neural network and pattern recognition technology are also used.The result of clinic application indicates that this instrument has certain reference value to the diagnosis of CAD and provides potential possibility of general examination.

To discuss the character and application of internal fixation with cervical lateral mass plate fixation in posterior approach of cervical spine operation. Twelve patients with cervical fracture dislocation treated with posterior cervical lateral mass fixation and bony fusion were selected. The patients received spinal decompression or reduction and decompression according to different diseases. A suitable 3.5 mm bi-cortical screw was inserted using Magerl technique. The results showed that solid bony fusion could be obtained at 3-5 months after the surgery, and the intervertebra heights as well as physiological curves were kept well without screw loosening or breaking. Neurological function was recovered to different extent. Cervical lateral mass plate fixation has the advantages of easily operation technique, wide indications, strong stability and short segment fixation.

Objective To compare the difference between self-made retractor and thyroid suspension in laparoscopic thyroidectomy,as well as exploring the feasibility and efficacy of self-made retractor.Methods Fifty nine thyroid patients were included in this study from Jan.2012 to Jun.2013 in our hospital.30 cases of laparoscopic thyroidectomy were completed by the help of self-made retractor while 29 cases were completed with thyroid suspension.The owration time,blood loss,drainage flow,hospital stays and short term effect were compared.Result The average operation time of retractor group (71.57 ± 9.56) min was shorter than the suspension group (79.90 ±10.61) min (P =0.002) The blood loss of retractor group (11.83 ±6.76) mL was less than the suspension group (17.24 ± 9.41) mL (P =0.014).The hospitalization time of retractor group (4.07 ± 0.45) days was similar with the suspension group (4.14 ± 0.52) min (P =0.573),The retractor group had shorter operation time,blood loss.There was statistical significance between them.There was no significance difference of hospital stays and discomfort.Neither group appeared of hoarseness,bucking,angulus oris numbness and other symptom.Conclusion Self-made retractor can reduce the operation time,blood loss.It is worth of clinical extension.

Objective To investigate the induction of specific anti-tumor immune response by transfected dendritic cells (DCs) with survivin mRNA of human pancreatic cancer, and to provide the experimental evidences for the treatment of human pancreatic cancer with DCs vaccine. Methods DCs were isolated and cultured from peripheral blood mononuclear cells (PBMCs). After being transcripted and amplified, survivin mRNA was transfected into DCs by electroporation. The expression of survivin in DCs at different time points was detected by quantitative real-time PCR. The survival rate of DCs before and after transfection was determined by MTT method. The induction of specific cytotoxic T lymphocyte (CTL) response by survivin mRNA transfected DCs was measured by 51Cr standard cytotoxicity test. The induction of specific CTL activation by survivin mRNA transfected DCs was evaluated through testing released IFN-γ by ELISA method. Results After survivin mRNA transfection for 48h, the expression of survivin mRNA in DCs reached the highest point (46.09±6.57). After transfection, the survival rate of DCs was stabilized around 80%. The DCs transfected with survivin mRNA could effectively induce HLA-A2+ / survivin+ specific CTL immune responses. Stimulated with pancreatic cancer cell line Capan-2 cells or SCL-1 cells as control group, the IFN-γ released in 24 hours by survivin specific CTL were (28.79±5.70) U/ml and (25.12±2.13) U/ml respectively, there was no significant difference (P=0.761). Conclusion The induction of CTLs by DCs transfected with human pancreatic cancer survivin mRNA could produce specific anti-tumor immunity.

Objective:To study the nursing care of multisite cardiac pacemaker implantation in treat cardiac arrhythmia and dilated cardiomyopathy.Methods:On the different basis of partient health problems,gave nursing care by apply nursing process.Carry out health edurcation in different period,so that improve the effect of health education and prevent the electrode break off.Results:(1)Au the pacemaker electrodes were at the good place,no broke off or moved away.The sense and the threshold were normal.(2)The patients' cardic function were improved and cardiac arrhythmia were disappear or improved.(3)The wound had no infection.(4)All the patients knew self-supervise and attention knowledge well.Conclusion:Applying nursing proless,keeping right position,keeping intensive observation and giving effective health education are the assurance for the mplantation.

Objective To investigate the role of mifepristone in endometriosis after laparoscopic surgery. Methods One hundred and forty-three women diagnosed with endometriosis after laparoscopic surgery were divided into two groups: one group with 78 cases were given mifepristone at 10 mg once daily (mifepristone group),and the other group with 65 cases were given gestrinone at 2.5 mg,twice every week (gestrinone group). All patients were followed-up 1 to 2 years and observed curative effect, compared the therapy effect on endometriosis as well as the side effects of two drugs. Results The clinical symptom and sign were improved at 3, 6, 12 months in two groups (P< 0.01). The rate of efficiency was 85.9%(67/78) in mifepristone group,and 84.6%(55/65) in gestrinone group, there was no significant difference between two groups (P > 0.05). The rates of acne, weight gain, joint ache, aminotransferase increase was lower in mifepristone group than those in gestrinone group (P <0.01 or <0.05). The recovery time of ovulation or menstruation was shorter in mifepristone group [(12.0 ± 2.9), (26.8 ± 3.9) d]than those in gestrinone group [(25.3 ±3.7), (35.0±4.1) d](P<0.05). Conclusions Mifepristone seems to be an effective,safe,and convenient treatment for endometriosis with low-cost and few side effects. Therefore,it can be used as first choice for treatment of endometriosis after laparoscopic surgery.

Objective To investigate the best method of transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into dendrtic cells (DCs). Methods DCs were cultured from peripheral blood mononuclear cells induced by rhGM-CSF, rhIL-4 and TNF-α. Morphology of DCs was observed. Flow cytometry was used to detect the mature DCs specific surface markers:CD40, HLA-DR, CD83, CD86. Mixed lymphocyte (MLR) was used to determine the ability of DCs to stimulate allogeneic T cell proliferation.Liposomal transfection, electroporation method and passive transfection was used to transfect MiaPaCa-2 cell total RNA into DCs, Real time RT-PCR and MTT assay was used to determine the expression of MUC1 mRNA and the survival rate of the RNA transfected DCs. Results The cells acquired showed typical DCs morphology, the positive rate of CD40, HLA DR, CD83 and CD86 were 34.3% ,50.2% ,89.2% and 73.6%,and they showed a strong ability to stimulate allogeneic T cell proliferation. 48 h after transfection with MiaPaCa-2 cells total RNA by using electroporation, the MUC1 mRNA amount (45.39 ± 9.33) in DCs was higher than that of liposomes method (3 1. 68 ± 7.25) and passive transfection method (18.53 ± 3.26) . DCs survival rate was (80.36 ± 2.43)% by using electroporation, which was relatively lower than (91.48 ±5.42) % by using passive transfection method, but higher than (67.44 ± 2.51) % by using liposomes method,and it was stabilized around 80%. Conclusions Transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into DCs with electroporation is efficient and safe.