Inflammatory Bowel Disease(IBD) is concerned with heredity,dysimmunity,environment and others,its characterized by chronic or relapsing inflammation within the gastrointestinal.IBD including two independent disease ulcerative colitis and crohn′s disease.In recent years with the incidence of IBD increased year by year,more women in reproductive age facing drug treatment.This article elucidated the effect of IBD and its treatment on perinatal.

Objective To investigate the causes of hearing loss for people over 60 years old. Methods People more than 60 years old in 14 provinces, 22 districts were investigated with a self-designed questionnaire, and a hearing test was also done for every subject. Results Among all the subjects, the etiological factors of hearing loss were otitis media (10.5%), hereditary deafness (2.9%), noise-induced hearing loss (3%), ototoxic deafness (6.4%), traumatic deafness (2%), presbycusis (33.8%), sudden deafness (1%) and other reasons (36.2%), respec-tively, with 11.3%unknown. Conclusion Presbycusis is the main reason of hearing loss for old people, and otitis media is the second. The cause of hearing loss is different with ages, districts, and whether in rural areas.

This report analyzes the status of schistosomiasis epidemic,surveillance and challenges in transmission?interrupt?ed areas of Jiangxi Province. There are 24 schistosomiasis transmission interrupted counties(cities,districts)among 39 endem?ic counties(cities,districts). Among the 24 counties,there are 6 counties with Oncomelania hupensis snails and 461 villages endemic,with 1.29 million residents. Since 2009,there have not been acute and chronic cases of schistosomiasis and all the pa?tients are historical advanced schistosomiasis cases. The area with snail habitats is 37.02 hm2. Since 2015,the schistosomiasis surveillance has covered the entire transmission interrupted areas. However,there are still many challenges,such as endemic in?stability,weak surveillance capacity,lack of surveillance awareness and other issues. Towards the schistosomiasis elimination, the surveillance should be based on the snail monitoring mainly,supplemented by the mobile population monitoring,and in ad?dition,the capacity building,standardized report of endemic,and snail habitat reform should also be further strengthened.

Objective To explore the effects of patient participation-based dietary intervention on nutritional status for patients with gastrectomy.Methods Fifty-two patients with gastrectomy were randomly assigned to the intervention group(n=26) and the control group(n=26).All patients were given general dietary guidance,and patients in the intervention group were provided patient participation-based dietary intervention(PPDI) as well.Height,body weight and body mass index(BMI) were recorded at baseline,2 weeks and 12 weeks after intervention,using PatientGenerated Subjective Global Assessment(PG-SGA),Diet-Related Symptom Scale,Adherence to Dietary Scale and a 3-day food diary.Results There was no obvious change in body weight and BMI before and after 12-week intervention,while outcomes in the control group gradually decreased.For time effect,there was significant difference in PG-SGA between two groups(P＜0.05).For between-group effect,there were significant differences in diet-related symptoms(P＜0.05),dietary intake(P＜0.05) and adherence to dietary(P＜0.05) between two groups.Conclusion Patient participation-based dietary intervention can improve nutritional status for patients with gastrectomy.

In order to have a better basic research of needle sticking, reports regarding basic research of needle sticking in recent years are arranged and summarized, including the concepts of needle sticking, the history origin, manipulation methods, precautions and selection requirements of needles. In the meanwhile, the reinforcing and reducing manipulation in sticking of the needle is preliminarily analyzed, and based on analysis of ancient records, three hypotheses are proposed: (1) needle sticking partly belongs to reinforcing method; (2) needle sticking partly belongs to reducing method; (3) needle sticking can perform reinforcing and reducing effects according to different manipulations. It is also believed that the needle sticking in modern clinical research is mostly used for reducing effects. However reinforcing and reducing manipulation in sticking of the needle still lacks of the support from ancient literature theory and modern clinical application, which needs to be improved.
Acupuncture Therapy ; instrumentation ; methods ; Humans ; Medicine in Literature ; Needles

Erythromycin A is a clinically important macrolide antibiotic with broad-spectrum activity. Its biosynthesis involves the formation of the 14-membered skeleton catalyzed by polyketide synthases, and the modification steps such as hydroxylation, glycosylation and methylation. Based on the understanding of the biosynthetic mechanism, it is reliable to genetically manipulate the erythromycin A-producing strain for production improvement and structure modification. In this paper, we reviewed the progress regarding erythromycin A in high-producing strain construction and chemical structure derivation, to provide insights for further development.
Anti-Bacterial Agents ; biosynthesis ; chemistry ; Erythromycin ; biosynthesis ; chemistry ; Glycosylation ; Hydroxylation ; Methylation ; Multigene Family ; Polyketide Synthases ; metabolism

Objective To study the clinical value of the combination detection of serum tumor markers alpha fetal protein hetero-geneity-L3(AFP-L3) ,Golgi protein 73(GP73) and phosphatidylinositol proteoglycan-3(GPC-3) in the diagnosis of primary hepatic cancer .Methods The serum AFP-L3 ,GP73 and GPC-3 levels were measured in 34 patients with primary hepatic cancer (PHC) ,20 patients with liver cirrhosis ,20 patients with chronic hepatitis B ,37 patients with other tumors by the enzyme linked immunosor-bent assay(ELISA) .Meanwhile ,20 individuals with healthy physical examination were selected as the control group .The expression situation of various indexes were compared and analyzed .Results The expression levels of AFP-L3 ,GP73 and GPC-3 in the PHC group were significantly higher than those in the control group and the other tumors groups ,difference was statistically significant (P<0 .05) .The areas under the receiver operating characteristic (ROC) curve of PHC in the single item detection of 3 markers were 0 .909 ,0 .832 and 0 .817 respectively .The areas of the ROC curve in the combination examinations of two items were 0 .935 , 0 .945 and 0 .912 respectively .The area of the ROC curve in the 3-item combined detection of PHC could be up to 0 .960 .Conclusion The combined detection of AFP-L3 ,GP73 and GPC-3 can increase the detective rate of PHC and has important clinical significance to early diagnosis of PHC .

Objective:To observe the effect of timosaponin AⅢon the proliferation of cultured melanoma B16 and A375 cells and nitrite produced from macrophage activation. Methods:MTT assay was adopted to detect the cell growth inhibition. The morphological changes of B16 and A375 cells were observed under an inverted microscope. Nitrite production of activated mouse macrophages induced by lipopolysaccharide ( LPS) plus IFN-γ was measured by Griess assay. Results: Timosaponin AⅢ could significantly inhibit the growth of B16 cells at the concentration of 16 μmol·L-1 after the 48- and 72-hour treatment, and significantly inhibit the growth of A375 cells at the concentration ranged from 4 to 16 μmol·L-1 after the 48-and 72-hour treatment. Shrinkage, vacuoles and necrosis of B16 and A375 cells were observed after the 48-and 72-hour treatment by 16μmol·L-1 timosaponin AⅢ,the other concentration of timosaponin AⅢ showed no notable effect on B16 cells and vacuoles of A375 cells appeared at the concentration from 4 to 16 μmol· L-1. Compared with RAW 264. 7 stimulated LPS plus IFN-γ,timosaponin AⅢ could significant inhibit nitrite production of macro-phage inflammatory cell model at the concentration of 10μmol·L-1(P<0. 01). Conclusion:Timosaponin AⅢcan inhibit the prolif-eration of melanoma B16 and A375 cells and macrophage inflammation,suggesting it has anti-tumor and anti-inflammatory effects . The anti-tumor effect of timosaponin AⅢ may be related to the inhibition of tumor inflammation.

OBJECTIVE To search for the reasons of unqualified level of bacteria in exit-entrance fluid of hemodialyzer and draft the measures of adjustment to meet the evaluation criteria for exit-entrance fluid of hemodialyzer.METHODS According to MOH′s Hospital Infection Management Standards to detect level and species of bacteria.RESULTS The average numbers of bacteria in exit-entrance fluid of hemodialyzer decreased from 2152.95?826.45 CFU/ml before adjustment to 579?541.04 CFU/ml after adjustment,checking with chi square test,the entrance fluid of hemodialyzer was ?2 =15.92,P

Objective To investigate the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft through the establishment of pancreatic cancer cell line SW -1990 subcutaneous xenograft model .Methods After pancreatic cancer xenograft in nude mice model beening established , All the mice were randomly divided into 4 groups and treated intraperitoneally ( IP) with saline and various concentrations (5,10,20 mg/kg) of ginsenosides Rg3.To observe the effect of ginsenoside Rg3 on tumor growth.Immunohisto-chemical-PAS staining was used to detect the effects of ginsenosides Rg 3 on vasculogenic mimicry of pancreatic cancer xenograft .and mRNA and protein expression of MMP 2、MMP9 were respectively evaluated by FQ -PCR and Western blot .Results The ginsenosides Rg3 can inhibit the growth of the tumor xenografts in nude mice .The inhibitory effect is the most obvious the 20 mg/kg of ginsenosides Rg3 group.The expression of MMP-2, MMP-9 were down-regulated compare with the control group , and the difference was signifi-cant;the Immunohistochemical -PAS staining showed the number of vasculogenic mimicry (+) and CD31 (+) were less than that in the control group .Conlusion Our results demonstrate that pancreatic vascular mimicry formation can be suppressed by Ginsenoside Rg 3 though reducing the expression of MMP -2, MMP-9 in our vivo experiments ,