BACKGROUND:Decidua tissue is one of the most important sources of endometrial stem cel s, from which stromal stem cel s can differentiate into various kinds of cel s under contain inductions. OBJECTIVE:To observe the differentiation of endometrial stromal stem cel s isolated from early-pregnancy decidua into endometrial epithelial cel s. METHODS:Endometrial stromal stem cel s from early-pregnancy decidua were cultured, identified and directional y induced. Then induced cel s were randomized into test group cultured in conditioned medium and control group cultured in normal culture medium containing fetal bovine serum. Expression of keratin was detected by immunofluorescence assay and western bolt assay in the two groups. RESULTS AND CONCLUSION:The endometrial stromal stem cel s from early-pregnancy decidua were successful y isolated and cultured, and presented with a S-shaped curve. Within 1-48 hours after inoculation, cultured cel s were in the adaptation phase;then, the cel s proliferated rapidly and began to grow logarithmical y at 48 hours after culture;after 60 hours of culture, the cel growth rate decreased, with a steady rise in the cel curve. Results from the flow cytometry detection showed that the endometrial stromal stem cel s could express CD73 and CD90, but not express CD34, CD45 and cytokeratin. Immunofluorescence findings showed the expression of keratin negative in the control group, and mostly positive in the test group. Besides, the relative expression of keratin in the test group was significantly higher than that in the control group (P<0.05). These results suggest that the endometrial stromal stem cel s can be isolated from the human decidua in early pregnancy, and differentiate into endometrial epithelial cel s by directional induction.

Arm lymphedema is a common and persistent complication of breast cancer surgery. Surgery or radiotherapy damages axillary lymphatic vessels and obstructs lymphatic reflux, leading to the accumulation of lymph fluid in the superficial soft tissues of the upper limbs and the subsequent formation of edema. Clinical treatment of breast cancer-related lymphedema remains a worldwide problem. Currently, there is still no cure.

OBJECTIVE: To observe the curative effect of Rifampicin Injection-Capsule sequential therapy for retreatment of smear positive pulmonary tuberculosis(TB) at intensive stage.METHODS: 80 patients with smear positive pulmonary tuberculosis at intensive stage were enrolled: 40(treatment group) were randomly assigned to receive HZES plus Rifampicin Injection-Capsule sequential therapy,and another 40(control group) to receive HZES plus Rifampicin capsule.Follow-up of sputum negative conversion rate,pulmonary lesion,and toxcity were scheduled at the end of first and second month during intensified stage.RESULTS: At the end of first and second month during intensified stage,the sputum negative conversion rates were 82.5% and 97.5% respectively in the treatment group versus 65.0% and 85.0% respectively in control gorup;the X-ray imaging test of chests revealed that the responsive rates were 17.5% and 40.0% in the treatment groups versus 10.0%and 25.0% in the control group.At the end of second month,the cavity closure(or diminution) rate was 41.7% in the treatment group versus 22.7% in the control group;the incidence of hepatic lesion was 22.5% verus 25.0% and that of gastrointestinal tract toxcity was 20.0% versus 45.0%.CONCLUSION: Rifampicin Injection-Capsule sequential therapy was proved to be of rapid onset of effect,remarkable efficacy,less toxicity and satisfactory efficacy in controlling symptom,shortening bacterial clearance time,reducing side effects and treating severe TB.

0.05),an effective mA seconds in the experimental group(296.74?19.15)mAs,than in the control group 350 mAs was reduced about 15.4%(P

As a new learning method,mind map presents learning materials in a visual,radical and non-linear way.Application of mind map in teaching of pathophysiology could help teachers get the thread of textbook,enhance students'motivation for learning,help students get the main points and improve their efficiency.Mind map could make students think in a way of problem-based learning,and its advantage in training up divergent thinking could also develop students'innovation ability.

Objective To investigate the variation of coagulation parameters in type 2 diabetes mellitus(T2DM)patients with microvascular complication. Methods Coagulation and fibrinolysis parameters were measured in 40 healthy controls (control group) and 80 T2DM patients (T2DM group) with (47 patients) and without (33 patients) microvasular complications. Results Compared with those in control group, the serum levels of fibrinogenand D-D dimmersin in T2DM group were found to be increased significantly:(4.29±1.70) mg/L vs. (3.12±0.49) mg/L, 0.395 (0.265-0.910) mg/L vs. 0.215 (0.163-0.300) mg/L, P<0.05;while the activity of antithrombin III and protein S levels were significantly decreased in T2DM group:(94.11±25.04)%vs. (103.90±12.48)%,(70.23±23.22)%vs. (90.35±17.35)%, P<0.05. Amongst the T2DM patients, the serum levels of APTT, fibrinogen,D-D dimmers, and the activity of protein S in patients with microvascular complication were found to be significantly higher than those in patients without microvascular complication:(38.09±5.73) s vs. (34.53±4.13) s,(4.60±1.93) mg/L vs.(3.86±1.21) mg/L, 0.630(0.320-1.200) mg/L vs 0.310(0.240-0.405) mg/L, (79.4± 22.16)%vs. (57.15±18.05)%, P<0.05. Conclusion Hypercoagulable state and decreased anti-coagulation ability may contribute to the risk of development of microvascular complication in T2DM patients.

Child ; Child, Preschool ; Enterovirus A, Human ; immunology ; isolation & purification ; Enterovirus Infections ; epidemiology ; physiopathology ; virology ; Hand, Foot and Mouth Disease ; epidemiology ; physiopathology ; virology ; Humans

Objective:To evaluate the clinical outcome of transcrestal maxillary sinus floor elevation(TMSFE)in the presence of pseudocyst.Methods:14 cases with pseudocyst were treated by TMSFE.In the operation following osteotome,pseudocyst was not ex-cised and sinus floor membrane was elevated,15 implants were placed for the 14 cases.Definite prosthesis was delivered about 6 months after implant placement.CBCT examination was done before,immediately after and 1-year after surgery,the cyst condition, the gain of vertical bone height and implant stability over time were observed.Results:Pre-operation residual ridge height of the alveo-lar crest was (6.85 ±1.07)mm.The sinus membrane was successfully elevated in all sites without perforation (mean elevation height,6.93 ±2.07)mm.All 15 implants were ossteointegrated.At 1-year revisit the gained bone height was (12.76 ±2.03)mm. Cyst grew bigger in 3 cases,not changed in 3 cases,became smaller in 5 cases and disappeared in 3 cases.Conclusion:Pseudocyst excision is not necessary in TMSFE.

Liver is the common metastatic site of breast cancer .The process of liver metastasis consists of multiple steps and involves various factors from breast cancer cells and the liver micro -environment .In this re-view,we reviewed the molecular(including the connection of proteins ,protein kinases,miRNAs),signal transduc-tion pathways(including CXCL12-CXCR4 axis,Wnt/beta-catenin signaling pathway,AF1q/TCF7/CD44 regu-latory axis,laminin receptor/Akt/ERK signaling pathway,leptin/ERK/IL-8 signaling pathway)and the influen-cing factors(including hypoxia inducible factors,tumor-associated fibroblasts,adipose tissue matrix stem cells, metalloproteinases,selective ligands,inflammatory cell infiltration)associated with the liver micro -environment in breast cancer .

Objective To calculate the single dose of traditional or modified trastuzumab to avoid mistakes during the course of drug making up.Methods Based on clearly relationship among the multiple parameters for the trastuzumab preparation,the trastuzumab bottle label is generated by means of the Excel form.Results There was no clinical case of using durgs errors happened during the course using among 280 patients.Conclusions The trastuzumab bottle label by means of the Excel form could improve the accuracy and safety of clinical use of trastuzumab.