OBJECTIVE:To explore a randomization-based method for calculation of the required drug amount dynamically allocated by central drugstore of each clinical trial site. METHODS: Using the method of mathematical modeling with multiple related factors considered,everyday stock and drug demand of each trial site were calculated,and the model for drug-allocation was built. RESULTS: Calculating daily drug amount based on a real accomplished clinical trial. The validation on the established mathematical model showed that the simulated results met our primary goals. CONCLUSION: Management of central drugstore is an essential link of central randomization system. Reasonable calculation of drug amount allocated to each center with the use of mathematical modeling enhances the level of scientific management of central drugstores and ensures the smooth proceeding of clinical trials.

AIM:To study cilncial features and significance of Vogt-Koyanagi - Harada syndrome ( VKHS ) by fundus fluorescein angiography ( FFA) .
METHODS: All 16 cases ( 30 eyes ) of VKHS were underwent by detailed paysical examination. Then took colour photographs and examined by FFA.
RESULTS:Uveitis stage of VKHS could be classified into three kinds of fundus appearances. The vision damage was mainly associated to exudation of choroidal vessel and exsudative retinal detachment rather than edema of optic disc which could achieve a better recovery.
CONCLUSION: FFA is an important and valuable examination to diagnose the VKHS forepart.

Humans ; Periodontal Diseases ; prevention & control ; therapy ; Translational Medical Research

Evidence-Based Medicine ; standards ; Humans ; Periodontal Diseases ; diagnosis ; therapy ; Research Design

OBJECTIVE:To provide reference for rational use of Fentanyl transdermal patch in the clinic. METHODS:By ex-tracting data from hospital information system and digital medical record management system,medical records of 150 cancer pain inpatients receiving Fentanyl transdermal patch were collected from our hospital during Jun.-Dec. 2014,and then investigated and analyzed retrospectively. RESULTS:Numeric rating scale (NRS) score of 150 cancer pain patients receiving Fentanyl transdermal patch were as follows:pain scores of 17 patients (11.3%) were 1-3 points,those of 63 patients (42.0%) were 4-6 points and those of 5 patients(3.3%)were 7-10 points;the rest of the patients were not rated. Among those 150 cases,88 cases were well tol-erated with opioid and 62 patients were considered to be opioid intolerant. Those intolerant patients were who could not be treated orally or could not bore side effects of opioid hepatic or renal dysfunction. 25 cases used Fentanyl transdermal patch without defi-nite evidence. As to drug combination,22 cases additionally received Morphine hydrochloride injection;12 cases additionally re-ceived Bucinnazine hydrochloride injection;8 cases additionally received Morphine tablet;10 cases additionally received Oxycodo-ne hydrochloride sustained-release tablets and Morphine hydrochloride sustained-release tablets;8 cases additionally received Oxyco-done and acetaminophen tablets;3 cases additionally received Carbamazepine tablets. CONCLUSIONS:Fentanyl transdermal patch are widely used for cancer pain treatment in our hospital. There still is irrational use of it,such as absence of pain score before use,no medication indication,irrational drug combination. Physicians should obey medication indication strictly,and clinical phar-macists should strengthen communication with physicians and pay attention to medication education for patients so as to guarantee rational drug use.

OBJECTIVE:To explore the pharmacological basis of Yang Xue Fu Kang granules on the treatment of postpartum persistent hemorrhage METHODS:The methods such as bleeding with cutting tail,glass capillary and recalcification time were used to observe the effects of Yang Xue Fu Kang granules on the bleeding time and the clotting time of animals RESULTS:For the mice suffering from experimental deficiency of both qi and blood in Chinese medicine,Yang Xue Fu Kang granules obviously reduced both the bleeding time of tail wounds and the clotting time tested with glass capillary Also the drug reduced the bleeding time of tail wound in normal rats and clotting time(recalcification time)in normal rabbits CONCLUSION:Among the mechanisms of Yang Xue Fu Kang granules on treating postpartum persistent hemorrhage,promoting coagulation function and enhancing stypticity possess some positions

This paper provides an insight into the "3+2" clinical teaching mode implemented from 2001,ponders on and explores related problems in the present mode and proposes the optimization strategy.

Objective: To observe the inhibitory effects of ING4 gene expression on the growth of MCF-7 cells. Methods: ING4 mRNA expression in three breast cancer lines was detected by real-time fluorogentic quantitative-PCR (RFQ-PCR). pcDNA3.1(+) ING4 was transfected into MCF-7 cells. The proliferation activity of MCF-7 cells was measured by MTT assay and flow cytometry. Cell apoptotic ratios were analyzed by Annexin-V/PI double staining. Results: The expression of ING4 was markedly lower in MCF-7, MDA-MB-435, and MDA-MB-231 cells compared with normal breast tissues. Over expression of ING4 slowed down proliferation speed of MCF-7 cells, increased the cell proportion in G1 phase and decreased the proportion in S phase (P < 0.05). The apoptotic ratio of MCF-7 cells was significantly increased compared to control group (P < 0.05). RT-PCR revealed that expressions of p21 and bax gene were all up-regulated but p53 had no change. Conclusion: ING4 was associated with tumorigenesis of breast cancer. Over expression of ING4 inhibited the proliferation of human breast cancer MCF-7 cells.

Adult ; Anti-Bacterial Agents ; administration & dosage ; Humans ; Male ; Periodontal Diseases ; therapy ; Subgingival Curettage ; Treatment Outcome

Anti-Infective Agents ; administration & dosage ; therapeutic use ; Dental Plaque ; therapy ; Humans ; Periodontal Diseases ; drug therapy